In Vivo Mercury Demethylation in a Marine Fish (Acanthopagrus schlegeli)

2017 ◽  
Vol 51 (11) ◽  
pp. 6441-6451 ◽  
Author(s):  
Xun Wang ◽  
Fengchang Wu ◽  
Wen-Xiong Wang
Keyword(s):  
Marine Fish ◽  
Acanthopagrus Schlegeli

scholarly journals Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion

2021 ◽  
Vol 18 (4) ◽  
pp. 1380
Author(s):  
Neusa Figueiredo ◽  
Beatriz Matos ◽  
Mário Diniz ◽  
Vasco Branco ◽  
Marta Martins
Keyword(s):  
Cell Viability ◽  
Cell Cultures ◽  
Marine Fish ◽  
Sparus Aurata ◽  
Cost Effective ◽  
Primary Hepatocyte ◽  
Digestion Method ◽  
Trypan Blue Exclusion ◽  
And Function

Primary cell cultures from wild organisms have been gaining relevance in ecotoxicology as they are considered more sensitive than immortalized cell lines and retain the biochemical pathways found in vivo. In this study, the efficacy of two methods for primary hepatocyte cell isolation was compared using liver from two marine fish (Sparus aurata and Psetta maxima): (i) two-step collagenase perfusion and (ii) pancreatin digestion with modifications. Cell cultures were incubated in L-15 medium at 17 ± 1 °C and monitored for up to six days for cell viability and function using the trypan blue exclusion test, MTT test, lactate dehydrogenase (LDH) activity, and ethoxyresorufin O-deethylase (EROD) activity after Benzo[a]Pyrene exposure. The results showed significant differences between the number of viable cells (p < 0.05), the highest number being obtained for the pancreatin digestion method (average = 4.5 ± 1.9 × 107 cells). Moreover, the hepatocytes showed solid adherence to the culture plate and the rounded shape, changing into a triangular/polygonal shape. The cell viability and function obtained by pancreatin digestion were maintained for five days, and the EROD induction after exposure to the B[a]P showed that cells were metabolically active. This study shows that the optimized pancreatin digestion method is a valid, cost-effective, and simple alternative to the standard perfusion method for the isolation of primary hepatocytes from fish and is suitable for ecotoxicological studies involving marine pollutants, such as PAHs.

scholarly journals Prolactin Production during Larval and Early Juvenile Periods of Euryhaline Marine Fish, Black Sea Bream Acanthopagrus schlegeli.

1991 ◽  
Vol 57 (10) ◽  
pp. 1833-1837 ◽  
Author(s):  
Ryo Kimura ◽  
Masaru Tanaka
Keyword(s):  
Black Sea ◽  
Marine Fish ◽  
Sea Bream ◽  
Early Juvenile ◽  
Acanthopagrus Schlegeli

In vivo studies of digestion and nutrient assimilation in marine fish larvae

Aquaculture ◽  
2001 ◽  
Vol 201 (1-2) ◽  
pp. 161-175 ◽  
Author(s):  
Ivar Rønnestad ◽  
Carlos R Rojas-Garcı́a ◽  
Sigurd K Tonheim ◽  
Luis E.C Conceição
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
In Vivo Studies ◽  
Nutrient Assimilation ◽  
Marine Fish Larvae

Development of a marine fish model for studying in vivo molecular responses in ecotoxicology

2008 ◽  
Vol 86 (2) ◽  
pp. 131-141 ◽  
Author(s):  
R.Y.C. Kong ◽  
J.P. Giesy ◽  
R.S.S. Wu ◽  
E.X.H. Chen ◽  
M.W.L. Chiang ◽  
...  
Keyword(s):  
Marine Fish ◽  
Molecular Responses ◽  
Fish Model

In vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, Acanthopagrus schlegeli

2004 ◽  
Vol 139 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Yan-Horn Lee ◽  
Jin-Lien Du ◽  
Yu-Su Shih ◽  
Shan-Ru Jeng ◽  
Lian-Tien Sun ◽  
...  
Keyword(s):  
Sex Steroids ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Black Porgy ◽  
Acanthopagrus Schlegeli ◽  
Hormone Content

Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection

2018 ◽  
Vol 216 ◽  
pp. 97-107 ◽  
Author(s):  
Yang Guanhua ◽  
Cheng Wang ◽  
Xuetong Wang ◽  
Ruiqing Ma ◽  
Huajun Zheng ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Marine Fish ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Vibrio Anguillarum ◽  
Transposon Mutagenesis ◽  
Fish Pathogen ◽  
Genome Wide

Effect of in vivo cadmium exposure on the respiratory burst of Marine Fish (Limanda limanda L.) phagocytes

1996 ◽  
Vol 41 (4) ◽  
pp. 327-342 ◽  
Author(s):  
Thomas H. Hutchinson ◽  
Margaret J. Manning
Keyword(s):  
Marine Fish ◽  
Respiratory Burst ◽  
Cadmium Exposure

Effects of tributyltin in vivo on hepatic cytochrome P450 forms in marine fish

1993 ◽  
Vol 24 (3-4) ◽  
pp. 219-240 ◽  
Author(s):  
Karl Fent ◽  
John J. Stegeman
Keyword(s):  
Cytochrome P450 ◽  
Marine Fish ◽  
Hepatic Cytochrome
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