R2R3-MYB Transcription Factor MdMYB73 Confers Increased Resistance to the Fungal Pathogen Botryosphaeria dothidea in Apples via the Salicylic Acid Pathway

2020 ◽  
Author(s):  
Kai-Di Gu ◽  
Quan-Yan Zhang ◽  
Jian-Qiang Yu ◽  
Jia-Hui Wang ◽  
Fu-Jun Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salicylic Acid ◽  
Fungal Pathogen ◽  
Myb Transcription Factor ◽  
Botryosphaeria Dothidea ◽  
Salicylic Acid Pathway ◽  
Acid Pathway ◽  
R2r3 Myb

scholarly journals R2R3-MYB Transcription Factor MdMYB73 Confers Increased Resistance to the Fungal Pathogen Botryosphaeria Dothidea via the Salicylic acid Pathway in Apple

2020 ◽  
Author(s):  
Kai-Di Gu ◽  
Quan-Yan Zhang ◽  
Jian-Qiang Yu ◽  
Jia-Hui Wang ◽  
Fu-Jun Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salicylic Acid ◽  
Myb Transcription Factor ◽  
Botryosphaeria Dothidea ◽  
Plant Materials ◽  
Positive Regulator ◽  
Ring Rot ◽  
Acid Pathway ◽  
R2r3 Myb ◽  
Apple Fruits

Abstract Background: The MYB transcription factor (TF) family is involved in many biological processes. However, the molecular mechanism of the MYB family in the resistance to apple ring rot remains poorly understood. Results: Here, the R2R3-MYB subfamily member MdMYB73 was cloned from “Royal Gala,” and its function was characterized by a positive regulator in controlling defense against Botryosphaeria dothidea. qRT-PCR and GUS staining analysis demonstrated that the expression of MdMYB73 was notably induced in apple fruits and transgenic calli after inoculation with B. dothidea. Overexpression of MdMYB73 enhanced resistance, while suppressing MdMYB73 expression weakened resistance to B. dothidea in apple calli and fruits. The increased resistance to B. dothidea was also observed in MdMYB73-expressing Arabidopsis thaliana. Interestingly, we found that MdMYB73 improved resistance against B. dothidea, possibly by the salicylic acid (SA) pathway, since SA content and expression of SA synthesis- and signaling-associated genes were higher in MdMYB73 overexpression plant materials compared with wild-type controls after inoculation. In addition, we discovered that MdMYB73 interacts with the B. dothidea positive regulator MdWRKY31 and together enhance the resistance to B. dothidea in apple. Conclusions: Overall, these findings shed light on the mechanism by which MdMYB73 enhances the resistance to B. dothidea, possibly by regulating the SA pathway.

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals Different Mechanisms Participate in the R-dependent Activity of the R2R3 MYB Transcription Factor C1

2004 ◽  
Vol 279 (46) ◽  
pp. 48205-48213 ◽  
Author(s):  
J. Marcela Hernandez ◽  
George F. Heine ◽  
Niloufer G. Irani ◽  
Antje Feller ◽  
Min-Gab Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
Dependent Activity ◽  
R2r3 Myb

An R2R3-MYB transcription factor CmMYB21 represses anthocyanin biosynthesis in color fading petals of chrysanthemum

2022 ◽  
Vol 293 ◽  
pp. 110674
Author(s):  
Yiguang Wang ◽  
Li-Jie Zhou ◽  
Yuxi Wang ◽  
Zhiqiang Geng ◽  
Baoqing Ding ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis ◽  
Myb Transcription Factor ◽  
Color Fading ◽  
R2r3 Myb

AgMYB12, a novel R2R3-MYB transcription factor, regulates apigenin biosynthesis by interacting with the AgFNS gene in celery

2021 ◽  
Author(s):  
Hao Wang ◽  
Jie-Xia Liu ◽  
Kai Feng ◽  
Tong Li ◽  
Ao-Qi Duan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
R2r3 Myb

scholarly journals A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

2018 ◽  
Vol 60 (2) ◽  
pp. 160-172 ◽  
Author(s):  
Xiao-Bo Zhang ◽  
Bao-Hua Feng ◽  
Hui-Mei Wang ◽  
Xia Xu ◽  
Yong-Feng Shi ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Salicylic Acid Pathway ◽  
Acid Pathway ◽  
Substitution Mutation

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

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