Molecular Characterization of a Mesophilic Cellobiose 2-Epimerase That Maintains a High Catalytic Efficiency at Low Temperatures

2021 ◽  
Author(s):  
Qiuming Chen ◽  
Yanchang Wu ◽  
Zhaolin Huang ◽  
Wenli Zhang ◽  
Wanmeng Mu
Keyword(s):  
Molecular Characterization ◽  
Low Temperatures ◽  
Catalytic Efficiency

Purification and characterization of a psychrophilic catalase from Antarctic Bacillus

2008 ◽  
Vol 54 (10) ◽  
pp. 823-828 ◽  
Author(s):  
Wei Wang ◽  
Mi Sun ◽  
Wanshun Liu ◽  
Bin Zhang
Keyword(s):  
Activation Energy ◽  
Low Temperatures ◽  
Catalytic Efficiency ◽  
Small Subunit ◽  
Purification And Characterization ◽  
Cold Adapted ◽  
Optimal Activity ◽  
Ph Range ◽  
Antarctic Seawater

Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 °C and at a pH range of 6–11. The enzyme could be inhibited by azide, hydroxylamine, and mercaptoethanol. These characteristics suggested that BNC is a small-subunit monofunctional catalase. The activation energy of BNC was 13 kJ/mol and the apparent kcat/Km values were 3.6 × 106 and 4 × 106 L·mol–1·s–1 at 4 and 25 °C, respectively. High catalytic efficiency of BNC at low temperatures enables this bacterium to scavenge H2O2 efficiently. BNC exhibited activation energy, catalytic efficiency, and thermostability comparable with some mesophilic homologues. Such similarity of enzymatic characteristics to mesophilic homologues, although uncommon among the cold-adapted enzymes in general, has also been observed in other psychrophilic small-subunit monofunctional catalases.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma

Molecular characterization of lincRNAs in Down syndrome associated leukemia

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann
Keyword(s):  
Down Syndrome ◽  
Molecular Characterization
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