Prospective Selective Mechanism of Emerging Senolytic Agents Derived from Flavonoids

Prolonged use of immunosuppressants with a selective mechanism of action is a promising strategy in the prevention of postoperative scarring in glaucoma surgery. In order to assess the cellular response of eye tissues to the implantation of bioresorbable drains saturated with cyclosporin A or everolimus, a filter-type hypotensive operation with implantation of polylactide-based drains was simulated in 12 rabbits. Drainages implanted in rabbits of the two experimental groups under study were pre-saturated with either cyclosporin A or everolimus. The control group consisted of animals that were implanted with drains not saturated with any drugs. On the 7th day after the operation, the animals were taken out of the experiment, the eyeballs were enucleated, and histological preparations stained with hematoxylin and eosin, as well as hematoxylin and Picrosirius-red were prepared. Using a score on a scale from 0 to 5, the cellular composition within the drainage material, the intensity of collagen synthesis in the drainage, the thickness of the capsule around the drainage, and the number of blood vessels were analyzed. In comparison with the control group, the study groups showed a significantly lower amount of mononuclear cells, fibroblasts and giant cells of foreign bodies, as well as a lower thickness of the capsules surrounding the drainage, up to their complete absence. In addition, the intensity of collagen synthesis inside the drainage material of the studied groups was significantly lower. The drains of the everolimus group were characterized by an extremely low density of viable cellular elements inside the implanted material and a complete absence of collagen. At the same time, no toxic effect of the substance on the surrounding tissues was found. Thus, the saturation of bioresorbable drainages based on polylactide with cyclosporin A and everolimus contributed to a decrease in the intensity of the formation of connective tissue elements both inside and around the drainage in the early postoperative period.

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Dynamic m6A methylation facilitates mRNA triaging to stress granules

Life Science Alliance ◽

10.26508/lsa.201800113 ◽

2018 ◽

Vol 1 (4) ◽

pp. e201800113 ◽

Cited By ~ 39

Author(s):

Maximilian Anders ◽

Irina Chelysheva ◽

Ingrid Goebel ◽

Timo Trenkner ◽

Jun Zhou ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Messenger Rna ◽

Stress Granules ◽

Rna Metabolism ◽

Methylation Pattern ◽

Domain Family ◽

Induced Stress ◽

And Function ◽

Selective Mechanism

Reversible post-transcriptional modifications on messenger RNA emerge as prevalent phenomena in RNA metabolism. The most abundant among them is N6-methyladenosine (m6A) which is pivotal for RNA metabolism and function; its role in stress response remains elusive. We have discovered that in response to oxidative stress, transcripts are additionally m6A modified in their 5′ vicinity. Distinct from that of the translationally active mRNAs, this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. These stress-induced newly methylated sites are selectively recognized by the YTH domain family 3 (YTHDF3) “reader” protein, thereby revealing a new role for YTHDF3 in shaping the selectivity of stress response. Our findings describe a previously unappreciated function for RNA m6A modification in oxidative-stress response and expand the breadth of physiological roles of m6A.

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Responses of Percid Fishes and Their Habitats to Eutrophication

Journal of the Fisheries Research Board of Canada ◽

10.1139/f77-263 ◽

1977 ◽

Vol 34 (10) ◽

pp. 1964-1971 ◽

Cited By ~ 70

Author(s):

J. H. Leach ◽

M. G. Johnson ◽

J. R. M. Kelso ◽

J. Hartmann ◽

W. Nümann ◽

...

Keyword(s):

Physicochemical Characteristics ◽

Habitat Modification ◽

Trophic Conditions ◽

Relative Level ◽

Cultural Eutrophication ◽

Fish Yield ◽

Species Dominance ◽

Wide Range ◽

Selective Mechanism ◽

The Continuum

Percid fishes are found in lakes that cover a wide range of trophic conditions. The responses of percids and their habitats to progressive cultural eutrophication are predictable. Alterations in physicochemical characteristics of habitats precipitate changes in phytoplankton, macrophytes, zooplankton, and benthos that are ultimately deleterious. Enrichment can lead to favorable responses in percids, but somewhere in the continuum of trophic conditions the responses become unfavorable. The relative level at which the response becomes negative varies with the species according to tolerances to altered environment, adaptabilities to new habitat and forage base, and reproductive behavior. Progressive eutrophication acts as a selective mechanism that leads to a predictable sequence of fish species. Other perturbations, such as exploitation and habitat modification, can act synergistically with cultural eutrophication in accelerating the sequence. As eutrophication proceeds, the succession of species may not lead to increased fish yield because part of the nutrient load may be channeled through unharvestable food chains. Key words: Percidae, habitat, eutrophication, species dominance, food habits, parasitism, disease

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Production of immunoreactive chorionic gonadotropin during mixed lymphocyte reactions: a possible selective mechanism for genetic diversity.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.83.18.6834 ◽

1986 ◽

Vol 83 (18) ◽

pp. 6834-6838 ◽

Cited By ~ 42

Author(s):

D. Harbour-McMenamin ◽

E. M. Smith ◽

J. E. Blalock

Keyword(s):

Genetic Diversity ◽

Chorionic Gonadotropin ◽

Mixed Lymphocyte Reactions ◽

Selective Mechanism

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Image Retrieval Using the Intensity Variation Descriptor

Mathematical Problems in Engineering ◽

10.1155/2020/6283987 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12 ◽

Cited By ~ 4

Author(s):

Zhao Wei ◽

Guang-Hai Liu

Keyword(s):

Image Retrieval ◽

Information Extraction ◽

Lateral Inhibition ◽

Intensity Variation ◽

Spatial Layout ◽

Inhibition Mechanism ◽

Square Root ◽

Optimal Direction ◽

Selective Mechanism ◽

Better Than

Variations between image pixel characteristics contain a wealth of information. Extraction of such cues can be used to describe image content. In this paper, we propose a novel descriptor, called the intensity variation descriptor (IVD), to represent variations in colour, edges, and intensity and apply it to image retrieval. The highlights of the proposed method are as follows. (1) The IVD combines the advantages of the HSV and RGB colour spaces. (2) It can simulate the lateral inhibition mechanism and orientation-selective mechanism to determine an optimal direction and spatial layout. (3) An extended weighted L1 distance metric is proposed to calculate the similarity of images. It does not require complex operations such as square or square root and leads to good performance. Comparative experiments on two Corel datasets containing 15,000 images show that the proposed method performs better than the SoC-GMM, CPV-THF, and STH methods and provides good matching of texture, colour, and shape.

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