Cytotoxicity and DNA Damage Caused from Diazinon Exposure by Inhibiting the PI3K-AKT Pathway in Porcine Ovarian Granulosa Cells

2018 ◽  
Vol 67 (1) ◽  
pp. 19-31 ◽  
Author(s):  
Wei Wang ◽  
Shi-Ming Luo ◽  
Jun-Yu Ma ◽  
Wei Shen ◽  
Shen Yin
Keyword(s):  
Dna Damage ◽  
Granulosa Cells ◽  
Akt Pathway ◽  
Ovarian Granulosa Cells

Dioscin Ameliorates Polycystic Ovary Syndrome by Inhibiting PI3K/Akt Pathway-Mediated Proliferation and Apoptosis of Ovarian Granulosa Cells

2020 ◽  
Vol 18 (4) ◽  
pp. 331-336
Author(s):  
Xinrong Li ◽  
Beili Lv ◽  
Haiyan Wang ◽  
Qiaohong Qian
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Western Blot ◽  
Granulosa Cells ◽  
Caspase 3 ◽  
Polycystic Ovary ◽  
Akt Pathway ◽  
Ovary Syndrome ◽  
Ovarian Granulosa Cells ◽  
Proliferation And Apoptosis ◽  
Induced Apoptosis

To understand the mechanism underlying Dioscin inhibition of polycystic ovary syndrome, we have examined its effects on ovarian granulosa cells from letrozole-treated rats. To this end, Western blot was utilized to determine changes in the levels of Bcl-2, cleaved caspase-3, caspase-3, and phosphatidylinositol 3-kinase (PI3K)/protein kinase B pathway after Dioscin treatment in letrozole-treated rats. Dioscin ameliorated polycystic ovary syndrome by reducing the serum level of testosterone and increasing progesterone levels. It also inhibited proliferation and induced apoptosis of ovarian granulosa cells in the rat model by decreasing the level of Bcl-2 and elevating cleaved caspase-3. Western blot analysis revealed that Dioscin suppressed the PI3K/Akt pathway by inhibiting p-AKT/AKT. SC79, a p-AKT/AKT activator, reversed the effects of Dioscin on the proliferation and apoptosis of ovarian granulosa cells. In conclusion, Dioscin might present a novel therapeutic opportunity for patients with polycystic ovary syndrome.

cDNA cloning reveals the potential of human ovarian granulosa cells to secrete collagenase inhibitor

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S138
Author(s):  
J. FREUDENSTEIN ◽  
J. MUCHA ◽  
G. RAPP ◽  
K. H. SHEIT
Keyword(s):  
Cdna Cloning ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Collagenase Inhibitor
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