Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-κB and p53 Pathways in Intestinal Epithelial Cells

Detachment-induced cell death (DICD) is considered to be one of the means by which intestinal epithelial cells (IEC) die of apoptosis as they reach the lumen and are shed. Caspases, a family of cysteine proteases, play a central role in initiating, amplifying, and executing apoptosis; however, the pattern of caspase activation in response to distinct apoptotic stimuli remains unknown. We investigated the kinetics of caspase activation during DICD in freshly isolated human IEC. DNA fragmentation is observed 90 min after detachment and is preceded by the sequential activation of preformed members of the CPP32 family of caspases. Activation of caspase 6 and cleavage of the endogenous caspase substrate poly(ADP-ribose) polymerase (EC 2.4.2.30 ) are detected within 15 min of detachment, 30–45 min before caspase 3 activation. Caspase 1 and caspase 10 are present as proenzymes, yet they remain inactive in response to this trigger of apoptosis. Human IEC are primed to rapidly undergo detachment-induced apoptosis involving the selective and sequential activation of preformed caspases. This study may enhance our understanding of physiological events occurring as IEC are shed. Their rapid apoptotic response to detachment may facilitate the high turnover of cells and ensure homeostasis in the intestinal epithelium.

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P0173 PP DELETION OF S-FIMBRIAE OPERON ABROGATES E. COLI K1 INDUCED APOPTOSIS IN INTESTINAL EPITHELIAL CELLS

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/00005176-200406001-00297 ◽

2004 ◽

Vol 39 (Supplement 1) ◽

pp. S124 ◽

Cited By ~ 1

Author(s):

M. M. Pietzak ◽

E. Chakraborty ◽

Y. Wang ◽

S. Huang

Keyword(s):

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial ◽

E Coli ◽

Induced Apoptosis

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Probiotic Bacterium Prevents Cytokine-induced Apoptosis in Intestinal Epithelial Cells

Journal of Biological Chemistry ◽

10.1074/jbc.m207050200 ◽

2002 ◽

Vol 277 (52) ◽

pp. 50959-50965 ◽

Cited By ~ 320

Author(s):

Fang Yan ◽

D. Brent Polk

Keyword(s):

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Probiotic Bacterium ◽

Intestinal Epithelial ◽

Induced Apoptosis

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Effects of Stearic Acid on Proliferation, Differentiation, Apoptosis, and Autophagy in Porcine Intestinal Epithelial Cells

Current Molecular Medicine ◽

10.2174/1566524019666190917144127 ◽

2020 ◽

Vol 20 (2) ◽

pp. 157-166

Author(s):

Yuan Yang ◽

Jin Huang ◽

Jianzhong Li ◽

Huansheng Yang ◽

Yulong Yin

Keyword(s):

Cell Differentiation ◽

Epithelial Cells ◽

Stearic Acid ◽

Er Stress ◽

Intestinal Epithelial Cells ◽

Dependent Manner ◽

Intestinal Epithelial ◽

High Concentrations ◽

Induced Apoptosis ◽

Dose Dependent

Background: Stearic acid (SA), a saturated long-chain fatty acid consisting of 18 carbon atoms, is widely found in feed ingredients, such as corn, soybeans, and wheat. However, the roles of SA in the renewal of intestinal epithelial cells remain unclear. Methods and Results: In the present study, we found that 0.01-0.1 mM SA promoted IPEC-J2 cell differentiation and did not affect IPEC-J2 cell viability. In addition, the results showed that the viability of IPEC-J2 cells was inhibited by SA in a time- and dose-dependent manner at high concentrations. Flow cytometry and western blot analysis suggested that SA induced apoptosis, autophagy and ER stress in cells. In addition, the amounts of triglyceride were significantly increased upon challenge with SA. Moreover, the decrease in the viability of cells induced by SA could be attenuated by 4-PBA, an inhibitor of ER stress. Conclusion: In summary, SA accelerated IPEC-J2 cell differentiation at 0.01-0.1 mM. Furthermore, SA induced IPEC-J2 cell apoptosis and autophagy by causing ER stress.

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