scholarly journals A Microwell–Printing Fabrication Strategy for the On-Chip Templated Biosynthesis of Protein Microarrays for Surface Plasmon Resonance Imaging

2016 ◽  
Vol 120 (37) ◽  
pp. 20984-20990 ◽  
Author(s):  
Gerald Manuel ◽  
Andrej Lupták ◽  
Robert M. Corn
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Microarrays ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
On Chip

scholarly journals Automatic Spot Identification Method for High Throughput Surface Plasmon Resonance Imaging Analysis

Biosensors ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 85 ◽  
Author(s):  
Zhiyou Wang ◽  
Xiaoqing Huang ◽  
Zhiqiang Cheng
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Protein Microarrays ◽  
Video Data ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Identification Method ◽  
Processing Techniques

An automatic spot identification method is developed for high throughput surface plasmon resonance imaging (SPRi) analysis. As a combination of video accessing, image enhancement, image processing and parallel processing techniques, the method can identify the spots in SPRi images of the microarray from SPRi video data. In demonstrations of the method, SPRi video data of different protein microarrays were processed by the method. Results show that our method can locate spots in the microarray accurately regardless of the microarray pattern, spot-background contrast, light nonuniformity and spotting defects, but also can provide address information of the spots.

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