Real-Time Monitoring of Azo Dye Interfacial Adsorption at Silica–Water Interface by Total Internal Reflection-Induced Surface Evanescent Wave

Langmuir ◽  
2018 ◽  
Vol 34 (26) ◽  
pp. 7612-7623 ◽  
Author(s):  
Yan Xiong ◽  
Qing Wang ◽  
Ming Duan ◽  
Jun Tan ◽  
Shenwen Fang ◽  
...  
Keyword(s):  
Real Time ◽  
Evanescent Wave ◽  
Azo Dye ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Water Interface ◽  
Real Time Monitoring ◽  
Interfacial Adsorption

Tracking of Nanoparticles Using Evanescent Wave Microscopy

Volume 4 ◽  
2004 ◽  
Author(s):  
Arindam Banerjee ◽  
Kenneth D. Kihm
Keyword(s):  
Diffusion Coefficient ◽  
Evanescent Wave ◽  
Total Internal Reflection ◽  
Tracer Particle ◽  
Three Dimensional ◽  
Internal Reflection ◽  
Brownian Diffusion ◽  
Wall Region ◽  
Identification Algorithm ◽  
Polystyrene Spheres

A novel three-dimensional tracking technique of nanoparticles in nanometer spatial resolution using a Ratiometric Total Internal Reflection Fluorescence Microscopy (R-TIRFM) is presented. Evanescent waves from the total internal reflection of an argon-ion laser (488 nm) are used to generate a thin sliced illumination field with its effective visualization range of 544-nm, equivalent to twice of the penetration depth of the evanescent wave field. Fluorescence-coated polystyrene spheres of 500-nm diameter (SG = 1.05) are used as tracers subjected to the Brownian diffusive motions. A ratiometric analysis of the fluorescence particle images together with a neural network particle-pair identification algorithm is used to track the tracer particle locations across multiple image frames in full three-dimensional ways. The techniques are used to examine the Brownian diffusive motion of nanoparticles as they approach the very near-wall region within a few hundred nanometers of a glass-water interface. The experimental results show that the measured Brownian diffusion coefficient is in good agreement with the theoretical hindered diffusion coefficient near a wall.

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