scholarly journals Live Imaging of Cellular Internalization of Single Colloidal Particle by Combined Label-Free and Fluorescence Total Internal Reflection Microscopy

2015 ◽  
Vol 12 (11) ◽  
pp. 3862-3870 ◽  
Author(s):  
Gerard D. Byrne ◽  
Driton Vllasaliu ◽  
Franco H. Falcone ◽  
Michael G. Somekh ◽  
Snjezana Stolnik
Keyword(s):  
Total Internal Reflection ◽  
Colloidal Particle ◽  
Live Imaging ◽  
Internal Reflection ◽  
Cellular Internalization ◽  
Label Free ◽  
Total Internal Reflection Microscopy

Label free imaging of cell-substrate contacts by holographic total internal reflection microscopy

2016 ◽  
Vol 10 (9) ◽  
pp. 1163-1170 ◽  
Author(s):  
Biagio Mandracchia ◽  
Oriella Gennari ◽  
Valentina Marchesano ◽  
Melania Paturzo ◽  
Pietro Ferraro
Keyword(s):  
Total Internal Reflection ◽  
Internal Reflection ◽  
Label Free ◽  
Cell Substrate ◽  
Total Internal Reflection Microscopy

Total internal reflection microscopy for live imaging of cellular uptake of sub-micron non-fluorescent particles

2008 ◽  
Vol 231 (1) ◽  
pp. 168-179 ◽  
Author(s):  
G. D. BYRNE ◽  
M. C. PITTER ◽  
J. ZHANG ◽  
F. H. FALCONE ◽  
S. STOLNIK ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Total Internal Reflection ◽  
Live Imaging ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy ◽  
Fluorescent Particles

scholarly journals Effect of Photon Counting Shot Noise on Total Internal Reflection Microscopy

Soft Matter ◽  
2021 ◽  
Author(s):  
Fan Cui ◽  
David J Pine
Keyword(s):  
Shot Noise ◽  
Total Internal Reflection ◽  
Colloidal Particle ◽  
Photon Counting ◽  
Internal Reflection ◽  
Scattering Intensity ◽  
Transparent Substrate ◽  
Total Internal Reflection Microscopy

Total internal reflection microscopy (TIRM) measures changes in the distance between a colloidal particle and a transparent substrate by measuring the scattering intensity of the particle illuminated by an evanescent...

Observation and control of thin-film defects using in-situ total-internal-reflection microscopy

1992 ◽  
Author(s):  
Forrest L. Williams ◽  
Gary A. Peterson, Jr. ◽  
Reed A. Schmell ◽  
Charles K. Carniglia
Keyword(s):  
Thin Film ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Microscopy ◽  
And Control

Measurement of Ca2+ signaling dynamics in exocrine cells with total internal reflection microscopy

2006 ◽  
Vol 291 (1) ◽  
pp. G146-G155 ◽  
Author(s):  
Jong Hak Won ◽  
David I. Yule
Keyword(s):  
Plasma Membrane ◽  
Total Internal Reflection ◽  
Acinar Cells ◽  
Internal Reflection ◽  
Pancreatic Acinar Cells ◽  
Wide Field ◽  
Exocrine Cells ◽  
Parotid Acinar Cells ◽  
Signaling Dynamics ◽  
Total Internal Reflection Microscopy

In nonexcitable cells, such as exocrine cells from the pancreas and salivary glands, agonist-stimulated Ca2+ signals consist of both Ca2+ release and Ca2+ influx. We have investigated the contribution of these processes to membrane-localized Ca2+ signals in pancreatic and parotid acinar cells using total internal reflection fluorescence (TIRF) microscopy (TIRFM). This technique allows imaging with unsurpassed resolution in a limited zone at the interface of the plasma membrane and the coverslip. In TIRFM mode, physiological agonist stimulation resulted in Ca2+ oscillations in both pancreas and parotid with qualitatively similar characteristics to those reported using conventional wide-field microscopy (WFM). Because local Ca2+ release in the TIRF zone would be expected to saturate the Ca2+ indicator (Fluo-4), these data suggest that Ca2+ release is occurring some distance from the area subjected to the measurement. When acini were stimulated with supermaximal concentrations of agonists, an initial peak, largely due to Ca2+ release, followed by a substantial, maintained plateau phase indicative of Ca2+ entry, was observed. The contribution of Ca2+ influx and Ca2+ release in isolation to these near-plasma membrane Ca2+ signals was investigated by using a Ca2+ readmission protocol. In the absence of extracellular Ca2+, the profile and magnitude of the initial Ca2+ release following stimulation with maximal concentrations of agonist or after SERCA pump inhibition were similar to those obtained with WFM in both pancreas and parotid acini. In contrast, when Ca2+ influx was isolated by subsequent Ca2+ readmission, the Ca2+ signals evoked were more robust than those measured with WFM. Furthermore, in parotid acinar cells, Ca2+ readdition often resulted in the apparent saturation of Fluo-4 but not of the low-affinity dye Fluo-4-FF. Interestingly, Ca2+ influx as measured by this protocol in parotid acinar cells was substantially greater than that initiated in pancreatic acinar cells. Indeed, robust Ca2+ influx was observed in parotid acinar cells even at low physiological concentrations of agonist. These data indicate that TIRFM is a useful tool to monitor agonist-stimulated near-membrane Ca2+ signals mediated by Ca2+ influx in exocrine acinar cells. In addition, TIRFM reveals that the extent of Ca2+ influx in parotid acinar cells is greater than pancreatic acinar cells when compared using identical methodologies.

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