Macromolecular Crowding and Confinement Effect on the Growth of DNA Nanotubes in Dextran and Hyaluronic Acid Media

2019 ◽  
Vol 3 (1) ◽  
pp. 412-420
Author(s):  
Qiufen Zhang ◽  
Qingwen Bai ◽  
Lin Zhu ◽  
Tianhao Hou ◽  
Jiang Zhao ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Macromolecular Crowding ◽  
Confinement Effect ◽  
Acid Media ◽  
Dna Nanotubes

Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution

2018 ◽  
Vol 20 (13) ◽  
pp. 8842-8847 ◽  
Author(s):  
Chiho Watanabe ◽  
Miho Yanagisawa
Keyword(s):  
Ethylene Glycol ◽  
Cell Size ◽  
Molecular Diffusion ◽  
Macromolecular Crowding ◽  
Confinement Effect ◽  
Protein Diffusion ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Glycol Solution ◽  
Size Confinement

Micrometric membrane confinements and macromolecular crowding synergistically regulate molecular diffusion.

Hyaluronic acid as a macromolecular crowding agent for production of cell-derived matrices

2019 ◽  
Vol 100 ◽  
pp. 292-305 ◽  
Author(s):  
D. Shendi ◽  
J. Marzi ◽  
W. Linthicum ◽  
A.J. Rickards ◽  
D.M. Dolivo ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Macromolecular Crowding

scholarly journals Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 859
Author(s):  
Sergio Garnica-Galvez ◽  
Stefanie H. Korntner ◽  
Ioannis Skoufos ◽  
Athina Tzora ◽  
Nikolaos Diakakis ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Cell Cultures ◽  
Hydrodynamic Radius ◽  
Negative Charge ◽  
Macromolecular Crowding ◽  
Molecular Weights ◽  
Tissue Equivalents ◽  
Collagen Iii ◽  
Adipose Derived Stem Cell

The use of macromolecular crowding in the development of extracellular matrix-rich cell-assembled tissue equivalents is continuously gaining pace in regenerative engineering. Despite the significant advancements in the field, the optimal macromolecular crowder still remains elusive. Herein, the physicochemical properties of different concentrations of different molecular weights hyaluronic acid (HA) and their influence on equine adipose-derived stem cell cultures were assessed. Within the different concentrations and molecular weight HAs, the 10 mg/mL 100 kDa and 500 kDa HAs exhibited the highest negative charge and hydrodynamic radius, and the 10 mg/mL 100 kDa HA exhibited the lowest polydispersity index and the highest % fraction volume occupancy. Although HA had the potential to act as a macromolecular crowding agent, it did not outperform carrageenan and Ficoll®, the most widely used macromolecular crowding molecules, in enhanced and accelerated collagen I, collagen III and collagen IV deposition.

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