Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering

2020 ◽  
Vol 12 (31) ◽  
pp. 34703-34715
Author(s):  
Joo Hee Choi ◽  
Namyoung Kim ◽  
Min A. Rim ◽  
Wonchan Lee ◽  
Jeong Eun Song ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gellan Gum ◽  
Osteochondral Tissue ◽  
Bone Particles ◽  
Osteochondral Tissue Engineering ◽  
Demineralized Bone

scholarly journals Gellan Gum-Based Hydrogel Bilayered Scaffolds for Osteochondral Tissue Engineering

2013 ◽  
Vol 587 ◽  
pp. 255-260 ◽  
Author(s):  
Diana R. Pereira ◽  
Raphael F. Canadas ◽  
Joana Silva-Correia ◽  
Alexandra P. Marques ◽  
Rui L. Reis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid Solution ◽  
Gellan Gum ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
X Ray ◽  
Hydrogel Scaffolds ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.

Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering

Biomaterials ◽  
2006 ◽  
Vol 27 (11) ◽  
pp. 2426-2433 ◽  
Author(s):  
Xudong Li ◽  
Li Jin ◽  
Gary Balian ◽  
Cato T. Laurencin ◽  
D. Greg Anderson
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering ◽  
Demineralized Bone

scholarly journals Silk Fibroin/Nano-CaP Bilayered Scaffolds for Osteochondral Tissue Engineering

2013 ◽  
Vol 587 ◽  
pp. 245-248 ◽  
Author(s):  
Le Ping Yan ◽  
Joaquim Miguel Oliveira ◽  
Ana L. Oliveira ◽  
Rui L. Reis
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid Solution ◽  
Single Layer ◽  
Bottom Layer ◽  
Apatite Crystal ◽  
Crystal Formation ◽  
Micro Computed Tomography ◽  
Computed Tomography Images ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering

In this study, bilayered silk and silk/nanoCaP scaffolds were developed for osteochondral tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nanoCaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed that the both layers presented porous structure and integrated well. Micro-computed tomography images confirmed that the CaP phase was only retained in the silk/nanoCaP layer. The hydration degree and mechanical properties of the bilayered scaffold were comparable to the ones of each single layer. The apatite crystal formation was limited to the silk/nanoCaP layer, when soaking the scaffold in a simulated body fluid solution, which is a must for the application of the developed scaffolds in OC tissue engineering.

scholarly journals Extrusion-based printing of poly(propylene fumarate) for osteochondral tissue engineering

2016 ◽  
Vol 4 ◽  
Author(s):  
Trachtenberg Jordan ◽  
Placone Jesse ◽  
Smith Brandon ◽  
Piard Charlotte ◽  
Fisher John ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Osteochondral Tissue ◽  
Poly Propylene ◽  
Osteochondral Tissue Engineering

Stem Cells in Osteochondral Tissue Engineering

2018 ◽  
pp. 359-372 ◽  
Author(s):  
Eleonora Pintus ◽  
Matteo Baldassarri ◽  
Luca Perazzo ◽  
Simone Natali ◽  
Diego Ghinelli ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering
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