Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid

2020 ◽  
Vol 12 (13) ◽  
pp. 15023-15033 ◽  
Author(s):  
Juan Han ◽  
Peng Luo ◽  
Lei Wang ◽  
Jiacong Wu ◽  
Chunmei Li ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Reaction System ◽  
Cascade Reaction ◽  
Efficient Conversion

Nanozyme-mediated cascade reaction system for ratiometric fluorescence detection of sarcosine

2021 ◽  
pp. 131341
Author(s):  
Wang Li ◽  
Tingting Li ◽  
Siqi Chen ◽  
Donglian Deng ◽  
Yibing Ji ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Reaction System ◽  
Cascade Reaction ◽  
Ratiometric Fluorescence

scholarly journals Biomimetic enzyme cascade reaction system in microfluidic electrospray microcapsules

2018 ◽  
Vol 4 (6) ◽  
pp. eaat2816 ◽  
Author(s):  
Huan Wang ◽  
Ze Zhao ◽  
Yuxiao Liu ◽  
Changmin Shao ◽  
Feika Bian ◽  
...  
Keyword(s):  
Reaction System ◽  
Cascade Reaction ◽  
Enzyme Cascade

Development of a novel bi-enzymatic silver dendritic hierarchical nanostructure cascade catalytic system for efficient conversion of starch into gluconic acid

2019 ◽  
Vol 356 ◽  
pp. 423-435 ◽  
Author(s):  
Saghar Rezaei ◽  
Amir Landarani–Isfahani ◽  
Majid Moghadam ◽  
Shahram Tangestaninejad ◽  
Valiollah Mirkhani ◽  
...  
Keyword(s):  
Catalytic System ◽  
Gluconic Acid ◽  
Hierarchical Nanostructure ◽  
Efficient Conversion

Cascade reaction system integrating nanozymes for colorimetric discrimination of organophosphorus pesticides

2021 ◽  
pp. 130810
Author(s):  
Haotian Zhong ◽  
Yuting Xue ◽  
Pengyu Zhang ◽  
Bin Liu ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Organophosphorus Pesticides ◽  
Reaction System ◽  
Cascade Reaction

Highly efficient conversion of microcrystalline cellulose to 5-hydroxymethyl furfural in a homogeneous reaction system

RSC Advances ◽  
2016 ◽  
Vol 6 (26) ◽  
pp. 21347-21351 ◽  
Author(s):  
Yan-Ru Zhang ◽  
Nan Li ◽  
Ming-Fei Li ◽  
Yong-Ming Fan
Keyword(s):  
Microcrystalline Cellulose ◽  
Zinc Chloride ◽  
Reaction System ◽  
Homogeneous Reaction ◽  
Hydroxymethyl Furfural ◽  
Efficient Conversion ◽  
Highly Efficient ◽  
System P ◽  
Chloride Hydrate

A homogenous reaction system with zinc chloride hydrate was explored for the synthesis of 5-HMF from MCC.

scholarly journals Biosynthesis of Raffinose and Stachyose from Sucrose via an In Vitro Multienzyme System

2018 ◽  
Vol 85 (2) ◽  
Author(s):  
Chaoyu Tian ◽  
Jiangang Yang ◽  
Yan Zeng ◽  
Tong Zhang ◽  
Yingbiao Zhou ◽  
...  
Keyword(s):  
Reaction System ◽  
Fold Increase ◽  
Cascade Reactions ◽  
Cascade Reaction ◽  
Galactinol Synthase ◽  
Plant Extraction ◽  
Cell Extracts ◽  
Multienzyme System ◽  
Raffinose Synthase

ABSTRACT Herein, we present a biocatalytic method to produce raffinose and stachyose using sucrose as the substrate. An in vitro multienzyme system was developed using five enzymes, namely, sucrose synthase (SUS), UDP-glucose 4-epimerase (GalE), galactinol synthase (GS), raffinose synthase (RS), and stachyose synthase (STS), and two intermedia, namely, UDP and inositol, which can be recycled. This reaction system produced 11.1 mM raffinose using purified enzymes under optimal reaction conditions and substrate concentrations. Thereafter, a stepwise cascade reaction strategy was employed to circumvent the instability of RS and STS in this system, and a 4.2-fold increase in raffinose production was observed. The enzymatic cascade reactions were then conducted using cell extracts to avoid the need for enzyme purification and supplementation with UDP. Such modification further increased raffinose production to 86.6 mM and enabled the synthesis of 61.1 mM stachyose. The UDP turnover number reached 337. Finally, inositol in the reaction system was recycled five times, and 255.8 mM raffinose (128.9 g/liter) was obtained. IMPORTANCE Soybean oligosaccharides (SBOS) have elicited considerable attention because of their potential applications in the pharmaceutical, cosmetics, and food industries. This study demonstrates an alternative method to produce raffinose and stachyose, which are the major bioactive components of SBOS, from sucrose via an in vitro enzyme system. High concentrations of galactinol, raffinose, and stachyose were synthesized with the aid of a stepwise cascade reaction process, which can successfully address the issue of mismatched enzyme characteristics of an in vitro metabolic engineering platform. The biocatalytic approach presented in this work may enable the synthesis of other valuable galactosyl oligosaccharides, such as verbascose and higher homologs, which are difficult to obtain through plant extraction.

Cascade Reaction System Integrating Single-Atom Nanozymes with Abundant Cu Sites for Enhanced Biosensing

2020 ◽  
Vol 92 (4) ◽  
pp. 3373-3379 ◽  
Author(s):  
Yu Wu ◽  
Jiabin Wu ◽  
Lei Jiao ◽  
Weiqing Xu ◽  
Hengjia Wang ◽  
...  
Keyword(s):  
Reaction System ◽  
Cascade Reaction ◽  
Single Atom

Catalytic deep eutectic solvents for highly efficient conversion of cellulose to gluconic acid with gluconic acid self-precipitation separation

2018 ◽  
Vol 54 (48) ◽  
pp. 6140-6143 ◽  
Author(s):  
Feijie Liu ◽  
Zhimin Xue ◽  
Xinhui Zhao ◽  
Hongyu Mou ◽  
Jing He ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Deep Eutectic Solvents ◽  
Efficient Conversion ◽  
Highly Efficient

FeCl3·6H2O based catalytic deep eutectic solvents were designed for the conversion of cellulose to gluconic acid with self-precipitation.

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