Beneficiation of Commiphora africana Plant: Extraction and Application of Green Softener on Cotton Fabric

This paper aims at the extraction and application of eco- and user-friendly natural gum obtained from Commiphora Africana tree. The result obtained is also compared with fabric treated with a commercial softener of the same concentration. The gum was extracted by puncturing the stem of the plant and the extracted gum was applied directly to 100% cotton fabric through a padding process with different concentrations of extracted gum (i.e., 20 gram per litre (g/l), 25 g/l, and 30 g/l). Another similar fabric sample was treated with a silicon softener of the same concentration. The fabric samples treated with both natural gum and silicon softener were tested for their stiffness, crease recovery, and drapability. The results show that the change in fabric softness depends on the concentration of softener used in both cases. As the concentration of the softener increased, there was a decrease in bending length and drape coefficient for both fabric samples. The drape coefficient of fabric sample treated with natural gum has a comparable result with fabric treated with silicon/commercial softener. Maximum increases in recovery angle were seen in fabric treated with natural gum at a concentration of 30 g/l.

Determination of Phytoextraction and Hyperaccumulating Capacity of Artemisa Dracunculus L and Erigeron Canadensis Plants of The Asteraceae Family.

One of its key aspects is the performance of plant extraction, the end-use of by-products and the recognition of its overall economic viability. Overall, phytoextraction seems to be a very promising technology for the removal of metallic pollutants from the environment and is being commercialized. In genetic engineering, support for the current plant phytoremedetion list plays a major role. Because it makes it possible to insert which genes in genetically modified plants the plant to metabolize a specific pollutant. In the sequence to be used for phytoremediation. In Turkey, the steel industry and its subindustries operate intensively in Karabuk province. It is located in the western region of the Black Sea and grew rapidly. For this reason, plants that grow in areas and soil samples around their roots are with anthropogenic pollution were taken in connection with the steel industry and the building industry where the waste is discharged. For guidance, samples of plants and soils of the same species were collected from orchards where industrial pollution and agricultural activity have not occurred, in order to worth comparing them. Thus, the properties of the hyperaccumulator and accumulation were investigated. These are Artemisa Dracunculus L (AD) and Erigeron Canadensis (EC) in the Asteraceae family. As the BAF shoot values of the AD plant are Pb and Se > 1, this is an accumulator plant for Pb and Se. At the EC plant, this is an accumulator for Pb, as only Pb>1. Since Cr, Hg, Sn and Cl <1, these elements are presumed to be exclusionary. Also, the two plants can be classified as potentially Ni-hyperaccumulatory plants because Ni is greater than 10 (Ni>10). BAF root of both plants has high phytostabilization capacity for CD from CD>1. The concentrations of Cd TF >1 in the leaf, stem and root indicate high phytoextraction efficiency and it can be said that it will exhibit high activity in soils contaminated by the CD. In addition, due to the fact that the Cd concentrations of both plants are close to the hyperaccumulator plant BAF, studies can be carried out to evaluate them as Cd-hyperaccumulator plant in future studies.

Effects of Mangrove Plant Extraction on Adhesion Strength in Coating of Epoxy

Steel is an alloy of ferrous metal with a maximum carbon content of 2%. This material is widely used for jetty building structures at rivers, estuary, and coastal areas. Corrosion of steel can occur due to several factors, such as the steel material itself and the surrounding environmental factors. One of the corrosion is biocorrosion that occurs due to the presence of biofouling. This study aims to determine the effect of adding extraction of mangrove (Avicennia marina) as an antifouling compound mixed in epoxy paint through adhesion strength parameter. Then the alloy was applied as a coating on the ASTM A36 steel specimen. The variation of coating composition were 100% of epoxy paint, 90%:10% (epoxy paint and extraction of Avicennia marina), 80% : 20% and 70% : 30%. The results showed that the adhesion strength occurred in 100% of epoxy paint (10.15 ± 0.64 Mpa). The adhesion strength was 8.86 ± 0.21 Mpa, 8.50 ± 0.03 Mpa, and 8.19 ± 0.07 Mpa at ratio of composition coating 90%: 10%, 80%: 20%, and 70%: 30%, respectively. In conclusion, the more mangrove extraction was added, the smaller the adhesion strength.

EFFICACY OF SEVELAMER CARBONATE IN COMBINATION WITH EDIBLE SOURCES IN HYPERPHOSPHATEMIA CONDITION ASSOCIATED WITH CHRONIC KIDNEY DISORDER

Objective: The main objective of the study was to discover a suitable dietary source of plant origin which can be recommended alongside the medication in case of hyperphosphatemia. Methods: The 2 plant samples chow chow (Sechiumedule) and bitter gourd (Momordica carantia) and two seed samples-Flax seeds (Linumusitatissimum) and Chia seeds (Salvia hispanica) were selected for the study. The samples were extracted using methanol. The filtrate obtained was used for dialysis studies under different conditions. Inorganic phosphate was estimated in each of the conditions. Results: The study indicated perturbation in the absorption of phosphate with the selected plant samples as talked about in the following study. The extreme concentration of phosphate among the four diverse picked plant sources was seen in bitter gourd, which was found to be 0.4686 µmoles/ml. A minimal measure of 0.0693 µmoles/ml of phosphate was found in chia seeds. Chow chow ended up being the best one in hyperphosphatemia condition as the amount of phosphate removed by it along with the drug, sevelamer carbonate, was calculated to be the maximum (0.0561 µmoles). It was followed by chia seeds (0.0429 µmoles) and flax seeds (0.0420 µmoles). Conclusion: The present study concentrates on the impact of various samples of plant extraction that can act on the phosphate absorption in the intestine by the procedure of dialysis.

EXTRACTION OF BIOACTIVE COMPONENTS OF KETEPENG CINA (Senna alata L.) LEAFS USING ULTRASONIC AND MACERATION METHODS ON VARIOUS SOLUTION CONCENTRATIONS

<p class="Abstrak"><span lang="EN-GB">Ketepeng cina <em>Senna alata</em> L. is one of the plant species growing in the tropical climate whose leaves have various bioactive components. It has been used as a traditional medicine to treat skin diseases. Ketepeng cina has been cultivated in several regions in Indonesia and the results are exported abroad to be used as medicinal raw materials. So far, the plant extraction process is still using the maceration method. This method is simple but has weaknesses in time consumption making it less efficient. The extraction method by ultrasonic waves promises more efficient extraction process than the maceration method. The purpose of this study was to determine the differences in extraction techniques and solvent concentration on the concentration of phytochemical constituents in ketepang cina leaves. The research design used a completely randomized design with two factorial patterns. The first factor was the technique of extracting Ketepeng cina leaves (maceration, ultrasonication), and the second factor was the concentration of solvent/ethanol (40%, 70%, 96%) The observation parameters included analysis of the content of total phenolic compounds, flavonoids, and tannins. The results showed that the use of the ultrasonication technique provided efficiency in extraction time compared to the maceration technique. The use of 70% ethanol resulted in higher concentrations of total phenolic and flavonoid compounds (28,17 mgGA/g) and (17,61 mgQE/g), but the highest concentration of tannins produced by 96% ethanol solvent was 6,78 mgAT/g. </span></p>

Antimicrobial Evaluation of Extracts and Fractions of Daniella oliveri Leaf on Selected Clinical Enteropathogens

Background: Diarrhoea is one of the major health threats to the populace in the tropics, and also one of the killer diseases in children under 5 years of age. Antimicrobial resistance and its spread pose serious public health threats, hence the need for development of safer and more effective antibacterial agents. Daniella oliveri Hutch and Dalz is used in ethnomedicine for the treatment of diarrhoea and other gastrointestinal disturbances. Objective: To investigate the antimicrobial activity of Daniella oliveri leaves on diarrhoeal pathogens. Methods: Successive plant extraction was carried out with hexane, ethylacetate and methanol using soxhlet apparatus. Methanol extract was fractionated using vacuum liquid chromatography (VLC). Phytochemical screening was done using standard chemical assays. Antibiogram of test isolates was carried out using disc diffusion assay. Antimicrobial activity of plant extract and fractions against strains of diarrhoeagenic Escherichia coli, Salmonella cholerae-suis, Shigella dysenteriae, Proteus mirabilis and Acinetobacter baumannii was determined by agar well diffusion and MIC by agar dilution methods. Kill-kinetics study was carried out using viable count technique. Results: Terpenoids, steroids and anthraquinones were detected in fractions of D. oliveri. Antibiogram assay showed that 66% of isolates were MDR. Extract and fractions produced appreciable zones of inhibition on all challenge organisms except Acinetobacter baumannii. MICs ranged between 6.25-25 mg/ml. Kill kinetics studies showed total kill on susceptible pathogens after 24 hours. Conclusion: This research has shown D. oliveri is a promising drug candidate for the management and treatment of diarrhoea.

Chemical Profile and Bioactivities of Extracts from Edible Plants Readily Available in Portugal

Plant extracts have been proposed as alternative biocides and antioxidants to be included in a variety of food products. In this work, to assess the potential of rosemary, lemon balm, basil, tarragon, sage, and spearmint to be used as food additives, the chemical profiles and bioactivities of such plant extracts were studied. Furthermore, to evaluate the influence of extraction methods and solvents on the chemical characteristics and bioactivities of the plant extracts, two extraction methods (solid-liquid and Soxhlet extraction) and two solvents (water and ethanol 70% (v/v)) were tested for each plant. Groupwise summary statistics were calculated by plant, extraction method, and solvent, and linear models were built to assess the main effects of those terms and their interactions on the chemical characteristics and bioactivities of the extracts. The results revealed that all factors—type of plant, extraction method and solvent—have influence on the chemical profile and antioxidant activity of the resultant extracts. Interactions between factors were also observed. Hydroethanolic Soxhlet extracts presented the least potential as biopreservatives due to their low phenolic content and reduced antioxidant capacity. Oppositely, aqueous Soxhlet extracts and hydroethanolic solid-liquid extracts showed high contents in phenolic compounds and high antioxidant activities. In particular, the hydroethanolic solid-liquid extracts of lemon balm, spearmint, and sage presented the highest phenolic and flavonoid contents, accompanied by a high antioxidant activity, and they revealed antimicrobial activity against four pathogens (S. enterica ser. Typhimurium, E. coli, L. monocytogenes and S. aureus). These results demonstrate the potential of these natural resources to be incorporated as bioactive preservatives in foods or their packaging.

Rhamnusalaternus Plant: Extraction of Bioactive Fractions and Evaluation of Their Pharmacological and Phytochemical Properties

Rhamnus alaternus, is a wild-growing shrub, belonging to the Rhamnaceae family. Widely distributed in the Mediterranean basin, R. alaternus is used in the usual medicine in numerous countries, mostly Tunisia, Algeria, Morocco, Spain, France, Italy, and Croatia. A large number of disorders—including dermatological complications, diabetes, hepatitis, and goiter problems—can be treated by the various parts of R. alaternus (i.e., roots, bark, berries, and leaves). Several bioactive compounds were isolated from R. alaternus, including flavonoids, anthocyanins, and anthraquinones, and showed several effects such as antioxidant, antihyperlipidemic, antigenotoxic, antimutagenic, antimicrobial, and antiproliferative. This review summarizes the updated information concerning the botanical description, distribution, extraction processes applied on R. alaternus, and its ethnopharmacology, toxicity, phytochemistry, and pharmacological effects.

Bioactive Phenolic Compounds from Primula veris L.: Influence of the Extraction Conditions and Purification

Our experiments may help to answer the question of whether cowslip (Primula veris L.) is a rich source of bioactive substances that can be obtained by efficient extraction with potential use as a food additive. A hypothesis assumed that the type of solvent used for plant extraction and the individual morphological parts of Primula veris L. used for the preparation of herbal extracts will have key impacts on the efficiency of the extraction of bioactive compounds, and thus, the health-promoting quality of plant concentrates produced. Most analysis of such polyphenolic compound contents in extracts from Primula veris L. has been performed by using chromatography methods such as ultra-performance reverse-phase liquid chromatography (UPLC−PDA−MS/MS). Experiments demonstrated that the most effective extraction agent for fresh study material was water at 100 °C, whereas for dried material it was 70% ethanol. The richest sources of polyphenolic compounds were found in cowslip primrose flowers and leaves. The aqueous and ethanol extracts from Primula veris L. were characterized by a quantitatively rich profile of polyphenolic substances, and a high antioxidative potential. Selective extraction with the use of mild conditions and neutral solvents is the first step to obtaining preparations from cowslip primrose with a high content of bioactive substances.

Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana

Camptothecin the third most in demand alkaloid, is commercially extracted in India from the endangered plant, Nothapodytes nimmoniana. Endophytes, the microorganisms that reside within plants, are reported to have the ability to produce host–plant associated metabolites. Hence, our research aims to establish a sustainable and high camptothecin yielding endophyte, as an alternative source for commercial production of camptothecin. A total of 132 endophytic fungal strains were isolated from different plant parts (leaf, petiole, stem and bark) of N. nimmoniana, out of which 94 were found to produce camptothecin in suspension culture. Alternaria alstroemeriae (NCIM1408) and Alternaria burnsii (NCIM1409) demonstrated camptothecin yields up to 426.7 ± 33.6 µg/g DW and 403.3 ± 41.6 µg/g DW, respectively, the highest reported production to date. Unlike the reported product yield attenuation in endophytes with subculture in axenic state, Alternaria burnsii NCIM1409 could retain and sustain the production of camptothecin up to ~ 200 μg/g even after 12 continuous subculture cycles. The camptothecin biosynthesis in Alternaria burnsii NCIM1409 was confirmed using 13C carbon labelling (and cytotoxicity analysis on different cancer cell lines) and this strain can now be used to develop a sustainable bioprocess for in vitro production of camptothecin as an alternative to plant extraction.