Gold Nanoparticles/Mo2C/MoO2-Modified Electrodes for Nucleic Acid Detection through CRISPR/Cas12a Photoelectrochemical Assay

2021 ◽  
Author(s):  
Nana Qin ◽  
Luping Deng ◽  
Mingjing Wang ◽  
Xu Hun
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Modified Electrodes ◽  
Nucleic Acid Detection ◽  
Photoelectrochemical Assay

Colorimetric Nucleic Acid Detection Based on Gold Nanoparticles with Branched DNA

NANO ◽  
2020 ◽  
Vol 15 (08) ◽  
pp. 2050110
Author(s):  
Zhikun Zhang ◽  
Xiaojie Ye ◽  
Qingqing Liu ◽  
Cuixia Hu ◽  
Jimmy Yun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Genetic Diseases ◽  
Colorimetric Detection ◽  
Nucleic Acid Detection ◽  
Great Promise ◽  
Dna Nanostructures ◽  
Practical Applications ◽  
Branched Dna ◽  
Target Dna

Nucleic acid detection is becoming increasingly important in the diagnostics of genetic diseases for biological analysis. We herein propose gold nanoparticles as probe for colorimetric detection of nucleic acids with branched DNA nanostructures, which enables a novel and simple colorimetric biosensor. In our system, the target DNA specifically triggered two short-chain ssDNA probes to generate branched DNA nanostructures (Y-shape DNA), which prevent AuNPs from aggregation in aqueous NaCl solution. On the contrary, when the target DNA did not exist, gold nanoparticles were unstable and aggregated easily because there is no anti-aggregation function from Y-shape DNA. Sensor response was found to be proportional to the target DNA concentration from 5 to 100[Formula: see text]nM, with detection limits determined as 5[Formula: see text]nM. The developed platform is for colorimetric nucleic acid detection without enzymes, label and modification holds great promise for practical applications.

Nucleic acid detection on DNA chips using highly reactive and stable diazo-biotins

2008 ◽  
Author(s):  
Alain Laurent ◽  
Arnaud Burr ◽  
Thibault Martin ◽  
Frédéric Lasnet ◽  
Sébastien Hauser ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Dna Chips

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

Open Medicine ◽  
2007 ◽  
Vol 2 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Koray Ergunay ◽  
Gulcin Altinok ◽  
Bora Gurel ◽  
Ahmet Pinar ◽  
Arzu Sungur ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
San Francisco ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Parvovirus B19 ◽  
Etiologic Agent ◽  
Hydrops Fetalis ◽  
Nucleic Acid Detection

AbstractIntrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).

Diagnosing Antibiotic Resistance Using Nucleic Acid Enzymes and Gold Nanoparticles

ACS Nano ◽  
2021 ◽  
Author(s):  
Mohamed A. Abdou Mohamed ◽  
Hannah N. Kozlowski ◽  
Jisung Kim ◽  
Kyryl Zagorovsky ◽  
Melinda Kantor ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antibiotic Resistance ◽  
Nucleic Acid
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