Photostable and Biocompatible Luminescent Thiol-Terminated Organosilica Nanoparticles with Embedded Au(I)–Thiolate Complexes for Fluorescent Microscopic Imaging

We describe the growth and characterization of highly fluorescing, near-infrared-emitting nanoclusters made of bimetallic Au25-xAgx cores, prepared using various monothiol-appended hydrophobic and hydrophilic ligands. The reaction uses well-defined triphenylphosphine-protected Au11 clusters (as precursors), which are reacted with Ag(I)-thiolate complexes. The prepared nanoclusters are small (diameter < 2nm, as characterized by TEM) with emission peak at 760 nm and long lifetime (~12 µs). The quantum yield measured for these materials was 0.3 - 0.4 depending on the ligand. XPS measurements show the presence of both metal atoms in the core, with measured binding energies that agree with reported values for nanocluster materials. The NIR emission combined with high quantum yield, small size and ease of surface functionalization afforded by the coating, make these materials suitable to implement investigations that address fundamental questions and potentially useful for biological sensing and imaging applications.

Download Full-text

Faculty Opinions recommendation of Multicolor and electron microscopic imaging of connexin trafficking.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1005811.76006 ◽

2002 ◽

Author(s):

Kermit Carraway

Keyword(s):

Electron Microscopic ◽

Microscopic Imaging

Download Full-text

Rapid identification of foodborne bacteria with hyperspectral microscopic imaging and artificial intelligent classification algorithms

Food Control ◽

10.1016/j.foodcont.2021.108379 ◽

2021 ◽

pp. 108379

Author(s):

Rui Kang ◽

Bosoon Park ◽

Qin Ouyang ◽

Ni Ren

Keyword(s):

Rapid Identification ◽

Classification Algorithms ◽

Artificial Intelligent ◽

Microscopic Imaging ◽

Foodborne Bacteria

Download Full-text

Video Light Microscopic Imaging of the Calcium Signal that Initiates Nuclear Envelope Breakdown in Sand Dollar (Echinaracnius parma) Cells

Biological Bulletin ◽

10.1086/bblv187n2p235 ◽

1994 ◽

Vol 187 (2) ◽

pp. 235-236 ◽

Cited By ~ 2

Author(s):

R. B. Silver

Keyword(s):

Nuclear Envelope ◽

Calcium Signal ◽

Sand Dollar ◽

Microscopic Imaging ◽

Nuclear Envelope Breakdown

Download Full-text

Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽

10.3390/cells10030499 ◽

2021 ◽

Vol 10 (3) ◽

pp. 499

Author(s):

Tracy W. Liu ◽

Seth T. Gammon ◽

David Piwnica-Worms

Keyword(s):

Molecular Imaging ◽

Single Cell ◽

Real Time ◽

Bioluminescence Imaging ◽

Emission Spectra ◽

Ccd Camera ◽

Microscopic Imaging ◽

Signaling Dynamics ◽

Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

Download Full-text