scholarly journals Pentavalent Sialic Acid Conjugates Block Coxsackievirus A24 Variant and Human Adenovirus Type 37–Viruses That Cause Highly Contagious Eye Infections

2020 ◽  
Vol 15 (10) ◽  
pp. 2683-2691
Author(s):  
Emil Johansson ◽  
Rémi Caraballo ◽  
Nitesh Mistry ◽  
Georg Zocher ◽  
Weixing Qian ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Eye Infections ◽  
Coxsackievirus A24 Variant ◽  
Coxsackievirus A24

scholarly journals Pentavalent Sialic Acid Conjugates Block Coxsackievirus A24 Variant and Human Adenovirus Type 37 – Viruses That Cause Highly Contagious Eye Infections

2020 ◽  
Author(s):  
Emil Johansson ◽  
Rémi Caraballo ◽  
Nitesh Mistry ◽  
Georg Zocher ◽  
Weixing Qian ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Agents ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Inhibitory Effect ◽  
Host Cells ◽  
Epidemic Keratoconjunctivitis ◽  
Coxsackievirus A24 Variant ◽  
Causative Agents ◽  
Coxsackievirus A24

Coxsackievirus A24 variant (CVA24v) and human adenovirus 37 (HAdV-37) are leading causative agents of the severe and highly contagious ocular infections acute hemorrhagic conjunctivitis and epidemic keratoconjunctivitis, respectively. Currently, neither vaccines nor antiviral agents are available for treating these diseases, which affect millions of individuals worldwide. CVA24v and HAdV-37 utilize sialic acid as attachment receptors facilitating entry into host cells. Previously, we and others have shown that derivatives based on sialic acid are effective in preventing HAdV-37 binding and infection of cells. Here, we designed and synthesized novel pentavalent sialic acid conjugates and studied their inhibitory effect against CVA24v and HAdV-37 binding and infection of human corneal epithelial cells. The pentavalent conjugates are the first reported inhibitors of CVA24v infection, and proved efficient in blocking HAdV-37 binding. Taken together, the pentavalent conjugates presented here form a basis for the development of general inhibitors of these highly contagious ocular pathogens.

scholarly journals Pentavalent Sialic Acid Conjugates Block Coxsackievirus A24 Variant and Human Adenovirus Type 37 – Viruses That Cause Highly Contagious Eye Infections

2020 ◽  
Author(s):  
Emil Johansson ◽  
Rémi Caraballo ◽  
Nitesh Mistry ◽  
Georg Zocher ◽  
Weixing Qian ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Agents ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Inhibitory Effect ◽  
Host Cells ◽  
Epidemic Keratoconjunctivitis ◽  
Coxsackievirus A24 Variant ◽  
Causative Agents ◽  
Coxsackievirus A24

Coxsackievirus A24 variant (CVA24v) and human adenovirus 37 (HAdV-37) are leading causative agents of the severe and highly contagious ocular infections acute hemorrhagic conjunctivitis and epidemic keratoconjunctivitis, respectively. Currently, neither vaccines nor antiviral agents are available for treating these diseases, which affect millions of individuals worldwide. CVA24v and HAdV-37 utilize sialic acid as attachment receptors facilitating entry into host cells. Previously, we and others have shown that derivatives based on sialic acid are effective in preventing HAdV-37 binding and infection of cells. Here, we designed and synthesized novel pentavalent sialic acid conjugates and studied their inhibitory effect against CVA24v and HAdV-37 binding and infection of human corneal epithelial cells. The pentavalent conjugates are the first reported inhibitors of CVA24v infection, and proved efficient in blocking HAdV-37 binding. Taken together, the pentavalent conjugates presented here form a basis for the development of general inhibitors of these highly contagious ocular pathogens.

scholarly journals Etiology of Acute Conjunctivitis Due to Coxsackievirus A24 Variant, Human Adenovirus, Herpes Simplex Virus, and Chlamydia in Beijing, China

2014 ◽  
Vol 67 (5) ◽  
pp. 349-355 ◽  
Author(s):  
Jie Li ◽  
Yongsheng Yang ◽  
Changying Lin ◽  
Weihong Li ◽  
Yang Yang ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Human Adenovirus ◽  
Coxsackievirus A24 Variant ◽  
Simplex Virus ◽  
Beijing China ◽  
Coxsackievirus A24 ◽  
Acute Conjunctivitis

scholarly journals Sialic Acid-Containing Glycans as Cellular Receptors for Ocular Human Adenoviruses: Implications for Tropism and Treatment

Viruses ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 395 ◽  
Author(s):  
Naresh Chandra ◽  
Lars Frängsmyr ◽  
Sophie Imhof ◽  
Rémi Caraballo ◽  
Mikael Elofsson ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Direct Interaction ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Epidemic Keratoconjunctivitis ◽  
Surface Plasmon Resonance Analysis ◽  
Cellular Receptors ◽  
Human Adenoviruses ◽  
Fiber Protein ◽  
Type 3

Human adenoviruses (HAdV) are the most common cause of ocular infections. Species B human adenovirus type 3 (HAdV-B3) causes pharyngoconjunctival fever (PCF), whereas HAdV-D8, -D37, and -D64 cause epidemic keratoconjunctivitis (EKC). Recently, HAdV-D53, -D54, and -D56 emerged as new EKC-causing agents. HAdV-E4 is associated with both PCF and EKC. We have previously demonstrated that HAdV-D37 uses sialic acid (SA)-containing glycans as cellular receptors on human corneal epithelial (HCE) cells, and the virus interaction with SA is mediated by the knob domain of the viral fiber protein. Here, by means of cell-based assays and using neuraminidase (a SA-cleaving enzyme), we investigated whether ocular HAdVs other than HAdV-D37 also use SA-containing glycans as receptors on HCE cells. We found that HAdV-E4 and -D56 infect HCE cells independent of SAs, whereas HAdV-D53 and -D64 use SAs as cellular receptors. HAdV-D8 and -D54 fiber knobs also bound to cell-surface SAs. Surprisingly, HCE cells were found resistant to HAdV-B3 infection. We also demonstrated that the SA-based molecule i.e., ME0462, designed to bind to SA-binding sites on the HAdV-D37 fiber knob, efficiently prevents binding and infection of several EKC-causing HAdVs. Surface plasmon resonance analysis confirmed a direct interaction between ME0462 and fiber knobs. Altogether, we demonstrate that SA-containing glycans serve as receptors for multiple EKC-causing HAdVs, and, that SA-based compound function as a broad-spectrum antiviral against known and emerging EKC-causing HAdVs.

scholarly journals Sialic Acid Is a Cellular Receptor for Coxsackievirus A24 Variant, an Emerging Virus with Pandemic Potential

2008 ◽  
Vol 82 (10) ◽  
pp. 5115-5115
Author(s):  
Emma C. Nilsson ◽  
Fariba Jamshidi ◽  
Susanne M. C. Johansson ◽  
M. Steven Oberste ◽  
Niklas Arnberg
Keyword(s):  
Sialic Acid ◽  
Cellular Receptor ◽  
Coxsackievirus A24 Variant ◽  
Emerging Virus ◽  
Coxsackievirus A24

scholarly journals Human adenovirus type 26 uses sialic acid–bearing glycans as a primary cell entry receptor

2019 ◽  
Vol 5 (9) ◽  
pp. eaax3567 ◽  
Author(s):  
Alexander T. Baker ◽  
Rosie M. Mundy ◽  
James A. Davies ◽  
Pierre J. Rizkallah ◽  
Alan L. Parker
Keyword(s):  
Sialic Acid ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Receptor ◽  
Primary Cell ◽  
Virus Infections ◽  
Epidemic Keratoconjunctivitis ◽  
Dna Viruses ◽  
Syncytial Virus ◽  
Entry Receptor

Adenoviruses are clinically important agents. They cause respiratory distress, gastroenteritis, and epidemic keratoconjunctivitis. As non-enveloped, double-stranded DNA viruses, they are easily manipulated, making them popular vectors for therapeutic applications, including vaccines. Species D adenovirus type 26 (HAdV-D26) is both a cause of EKC and other diseases and a promising vaccine vector. HAdV-D26–derived vaccines are under investigation as protective platforms against HIV, Zika, and respiratory syncytial virus infections and are in phase 3 clinical trials for Ebola. We recently demonstrated that HAdV-D26 does not use CD46 or Desmoglein-2 as entry receptors, while the putative interaction with coxsackie and adenovirus receptor is low affinity and unlikely to represent the primary cell receptor. Here, we establish sialic acid as a primary entry receptor used by HAdV-D26. We demonstrate that removal of cell surface sialic acid inhibits HAdV-D26 infection, and provide a high-resolution crystal structure of HAdV-D26 fiber-knob in complex with sialic acid.

scholarly journals Sialic Acid Is a Cellular Receptor for Coxsackievirus A24 Variant, an Emerging Virus with Pandemic Potential

2008 ◽  
Vol 82 (6) ◽  
pp. 3061-3068 ◽  
Author(s):  
Emma C. Nilsson ◽  
Fariba Jamshidi ◽  
Susanne M. C. Johansson ◽  
M. Steven Oberste ◽  
Niklas Arnberg
Keyword(s):  
Sialic Acid ◽  
Influenza A ◽  
Cellular Receptor ◽  
Virus Binding ◽  
Corneal Epithelial ◽  
Coxsackievirus A24 Variant ◽  
Emerging Virus ◽  
Enterovirus Type ◽  
Protease Treatment ◽  
Coxsackievirus A24

ABSTRACT Binding to target cell receptors is a critical step in the virus life cycle. Coxsackievirus A24 variant (CVA24v) has pandemic potential and is a major cause of acute hemorrhagic conjunctivitis, but its cellular receptor has hitherto been unknown. Here we show that CVA24v fails to bind to and infect CHO cells defective in sialic acid expression. Binding of CVA24v to and infection of corneal epithelial cells are efficiently inhibited by treating cells with a sialic acid-cleaving enzyme or sialic acid-binding lectins and by treatment of the virus with soluble, multivalent sialic acid. Protease treatment of cells efficiently inhibited virus binding, suggesting that the receptor is a sialylated glycoprotein. Like enterovirus type 70 and influenza A virus, CVA24v can cause pandemics. Remarkably, all three viruses use the same receptor. Since several unrelated viruses with tropism for the eye use this receptor, sialic acid-based antiviral drugs that prevent virus entry may be useful for topical treatment of such infections.

scholarly journals Coxsackievirus A24 Variant Uses Sialic Acid-Containing O-Linked Glycoconjugates as Cellular Receptors on Human Ocular Cells

2011 ◽  
Vol 85 (21) ◽  
pp. 11283-11290 ◽  
Author(s):  
N. Mistry ◽  
H. Inoue ◽  
F. Jamshidi ◽  
R. J. Storm ◽  
M. S. Oberste ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cellular Receptors ◽  
Coxsackievirus A24 Variant ◽  
Coxsackievirus A24
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