Real-Time Imaging and Quantification of Peptide Uptake in Vitro and in Vivo

2019 ◽  
Author(s):  
Hacer Karatas ◽  
Tamara Maric ◽  
Pier Luca D’Alessandro ◽  
Aleksey Yevtodiyenko ◽  
Thomas Vorherr ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Imaging ◽  
Peptide Uptake

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

scholarly journals Real-time imaging of mast cell degranulation in vitro and in vivo

2016 ◽  
Vol 479 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Kayo Horiguchi ◽  
Soichiro Yoshikawa ◽  
Asuka Saito ◽  
Salma Haddad ◽  
Takuya Ohta ◽  
...  
Keyword(s):  
Mast Cell ◽  
Real Time ◽  
Mast Cell Degranulation ◽  
Real Time Imaging

In vitro and in vivo real-time imaging with ultrasonic limited diffraction beams

1993 ◽  
Vol 12 (4) ◽  
pp. 819-829 ◽  
Author(s):  
Jian-yu Lu ◽  
T.K. Song ◽  
R.R. Kinnick ◽  
J.F. Greenleaf
Keyword(s):  
Real Time ◽  
Real Time Imaging

Abstract 1385: Real-time imaging of paxillin-GFP expression in breast cancer cells in vitro and in vivo

2011 ◽  
Author(s):  
Atsushi Suetsugu ◽  
Michelle Digman ◽  
Enrico Gratton ◽  
Hisataka Moriwaki ◽  
Shigetoyo Saji ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Breast Cancer Cells ◽  
Gfp Expression ◽  
Real Time Imaging

scholarly journals Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

2009 ◽  
Vol 3 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Paul Timpson ◽  
Alan Serrels ◽  
Marta Canel ◽  
Margaret C. Frame ◽  
Valerie G. Brunton ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Real Time ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Real Time Imaging

scholarly journals Transient ICD malfunctions during oncologic radiotherapy: a multi-centre, randomized, in-vitro study

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
E Di Girolamo ◽  
M Appignani ◽  
N Furia ◽  
M Marini ◽  
P De Filippo ◽  
...  
Keyword(s):  
Real Time ◽  
Treatment Plan ◽  
Low Energy ◽  
In Vivo Dosimetry ◽  
Implantable Cardioverter Defibrillators ◽  
Shock Delivery ◽  
Transient Electromagnetic ◽  
Direct Exposure

Abstract Background Direct exposure of implantable cardioverter-defibrillators (ICDs) during radiotherapy is still considered potentially harmful, or even unsafe, by manufacturers and current recommendations. The effects of photon beams on ICDs are unpredictable, depending on multiple factors, and malfunctions may present during exposure. Purpose To evaluate transient ICD malfunctions by direct exposure to doses up to 10 Gy during low-energy RT, forty-three contemporary wireless-enabled ICDs, with at least 4 months to elective replacement indicator (ERI) were evaluated in a real-time in-vitro session in three different centres. Methods All ICDs had baseline interrogation. Single chamber devices were programmed to the VVI/40 mode and dual or triple chamber devices were programmed to the DDD/40 mode. Rate response function and antitachycardia therapies were disabled, with the ventricular tachycardia (VT)/ventricular fibrillation (VF) detection windows still active. A centring computed tomography was performed to build the corresponding treatment plan and the ICDs were blinded randomized to receive either 2-, 5- or 10-Gy exposure by a low photon-energy linear accelerator (6MV) in a homemade water phantom (600 MU/min). The effective dose received by the ICDs was randomly assessed by an in-vivo dosimetry. During radiotherapy, the ICDs were observed in a real-time session using manufacturer specific programmer, and device function (pacing, sensing, programmed parameters, arrhythmia detections) was recorder by the video camera in the bunker throughout the entire photon exposure. All ICDs had an interrogation session immediately after exposure. Results During radiotherapy course, almost all ICDs (93%) recorded major or minor transient electromagnetic interferences. On detail, sixteen ICDs (37.2%) reported atrial and/or ventricular oversensing, with base-rate-pacing inhibition and VT/VF detection. Twenty-four ICDs (55.8%) recorded non clinically relevant noise, and no detections were observed. Only three ICDs (7%) reported neither transient malfunction nor minor noise, withstanding direct radiation exposure. At immediate post-exposure interrogation, the ICDs that recorded major real-time malfunctions had VT/VF detections stored in the device memory. In none of the ICDs spontaneous changes in parameter settings were reported. Malfunctions occurred regardless of either 2-, 5- or 10-Gy photon beam exposure. Conclusions Transient electromagnetic interferences were observed in most of the contemporary ICDs during radiotherapy course, regardless of photon dose. To avoid potentially life-threatening ICD malfunctions such as pacing inhibition or inappropriate shock delivery, magnet application on the pocket site or ICD reprogramming to the asynchronous mode are still suggested in ICD patients ongoing even low energy radiotherapy exposure. Funding Acknowledgement Type of funding source: None

Facile solvothermal synthesis of ultrathin spinel ZnMn2O4 nanospheres: An efficient electrocatalyst for in vivo and in vitro real time monitoring of H2O2

2021 ◽  
Vol 900 ◽  
pp. 115674
Author(s):  
Muthaiah Annalakshmi ◽  
Sakthivel Kumaravel ◽  
T.S.T. Balamurugan ◽  
Shen-Ming Chen ◽  
Ju-Liang He
Keyword(s):  
Real Time ◽  
Solvothermal Synthesis ◽  
Real Time Monitoring
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