Liposomes Coated with Isolated Macrophage Membrane Can Target Lung Metastasis of Breast Cancer

Background: CDK12 is a promising therapeutic target in breast cancer with an effective ability of maintaining cancer cell stemness. Objective: We aim to investigate the mechanism of CDK12 in maintaining breast cancer stemness. Methods: CDK12 expression level was accessed by using RT-qPCR and IHC. CDK12-altered breast cancer cell lines MDA-MB-231-shCDK12 and SkBr-3-CDK12 were then established. CCK8, colony formation assays, and xenograft model were used to value the effect of CDK12 on tumorigenicity. Transwell assay, mammosphere formation, FACS, and lung metastasis model in vivo were determined. Western blot further characterized the mechanism of CDK12 in breast cancer stemness through the c-myc/β-catenin pathway. Results: Our results showed a higher level of CDK12 exhibited in breast cancer samples. Tumor formation, cancer cell mobility, spheroid forming, and the epithelial-mesenchymal transition will be enhanced in the CDK12high group. In addition, CDK12 was associated with lung metastasis and maintained breast cancer cell stemness. CDK12high cancer cells presented higher tumorigenicity and a population of CD44+ subset compared with CDK12low cells. Our study demonstrated c-myc positively expressed with CDK12. The c-myc/β-catenin signaling was activated by CDK12, which is a potential mechanism to initiate breast cancer stem cell renewal and may serve as a potential biomarker of breast cancer prognosis. Conclusion: CDK12 overexpression promotes breast cancer tumorigenesis and maintains the stemness of breast cancer by activating c-myc/β-catenin signaling. Inhibiting CDK12 expression may become a potential therapy for breast cancer.

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Entelon® (Vitis vinifera Seed Extract) Prevents Cancer Metastasis via the Downregulation of Interleukin-1 Alpha in Triple-Negative Breast Cancer Cells

Molecules ◽

10.3390/molecules26123644 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3644

Author(s):

Daeun You ◽

Yisun Jeong ◽

Sun Young Yoon ◽

Sung A Kim ◽

Eunji Lo ◽

...

Keyword(s):

Breast Cancer ◽

Signaling Pathway ◽

Lung Metastasis ◽

Triple Negative Breast Cancer ◽

Cancer Metastasis ◽

Triple Negative ◽

Interleukin 1 ◽

Metastatic Potential ◽

Cancer Cell Proliferation ◽

Dependent Pathway

Interleukin-1 (IL1) is a proinflammatory cytokine and promotes cancer cell proliferation and invasiveness in a diversity of cancers, such as breast and colon cancer. Here, we focused on the pharmacological effect of Entelon® (ETL) on the tumorigenesis of triple-negative breast cancer (TNBC) cells by IL1-alpha (IL1A). IL1A enhanced the cell growth and invasiveness of TNBC cells. We observed that abnormal IL1A induction is related with the poor prognosis of TNBC patients. IL1A also increased a variety of chemokines such as CCL2 and IL8. Interestingly, IL1A expression was reduced by the ETL treatment. Here, we found that ETL significantly decreased the MEK/ERK signaling pathway in TNBC cells. IL1A expression was reduced by UO126. Lastly, we studied the effect of ETL on the metastatic potential of TNBC cells. Our results showed that ETL significantly reduced the lung metastasis of TNBC cells. Our results showed that IL1A expression was regulated by the MEK/ERK- and PI3K/AKT-dependent pathway. Taken together, ETL inhibited the MEK/ERK and PI3K/AKT signaling pathway and suppressing the lung metastasis of TNBC cells through downregulation of IL1A. Therefore, we propose the possibility of ETL as an effective adjuvant for treating TNBC.

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Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis

Journal of Molecular Medicine ◽

10.1007/s00109-011-0855-y ◽

2012 ◽

Vol 90 (7) ◽

pp. 803-815 ◽

Cited By ~ 129

Author(s):

Carmen Chak-Lui Wong ◽

Huafeng Zhang ◽

Daniele M. Gilkes ◽

Jasper Chen ◽

Hong Wei ◽

...

Keyword(s):

Breast Cancer ◽

Lung Metastasis ◽

Hypoxia Inducible Factor ◽

Metastatic Niche ◽

Hypoxia Inducible Factor 1 ◽

Niche Formation

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Effects of PEGylated paclitaxel nanocrystals on breast cancer and its lung metastasis

Nanoscale ◽

10.1039/c4nr07450e ◽

2015 ◽

Vol 7 (24) ◽

pp. 10790-10800 ◽

Cited By ~ 44

Author(s):

Hua Zhang ◽

Hongxiang Hu ◽

Haoran Zhang ◽

Wenbing Dai ◽

Xinglin Wang ◽

...

Keyword(s):

Breast Cancer ◽

Lung Metastasis

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The Measure of DAMPs and a role for S100A8 in recruiting suppressor cells in breast cancer lung metastasis

Immunological Investigations ◽

10.3109/08820139.2014.952818 ◽

2014 ◽

Vol 44 (2) ◽

pp. 174-188 ◽

Cited By ~ 12

Author(s):

Christine N. Vrakas ◽

Ryan M. O’Sullivan ◽

Samantha E. Evans ◽

DaMarcus A. Ingram ◽

Carli B. Jones ◽

...

Keyword(s):

Breast Cancer ◽

Lung Metastasis ◽

Suppressor Cells

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Co‑expression Network Analysis by WGCNA and Identify Potential Prognostic Markers Associated with Lung Metastasis in Breast Cancer

10.21203/rs.3.rs-182567/v1 ◽

2021 ◽

Author(s):

xixun zhang

Keyword(s):

Breast Cancer ◽

Network Analysis ◽

Lung Metastasis ◽

Prognostic Markers ◽

Clinical Information ◽

Preventive Treatment ◽

The Cancer Genome Atlas ◽

Functional Enrichment ◽

Breast Cancer Patients ◽

Kaplan Meier

Abstract Backgroud: Breast cancer (BC) is an aggressive cancer with a high percentage recurrence and metastasis. As one of the most common distant metastasis organ in breast cancer, lung metastasis has a worse prognosis than that of liver and bone. Therefore, it’s important to explore some potential prognostic markers associated with the lung metastasis in breast cancer for preventive treatment. Methods: In our study, transcriptomic data and clinical information of breast cancer patients were downloaded from The Cancer Genome Atlas (TCGA) database. Co-expression modules was built by Weighted gene co-expression network analysis (WGCNA) to find out the royalbule modules which is significantly associated with lung metastasis in breast cancer. Then, co-expression genes were analyzed for functional enrichment. Furthermore, the prognostic value of these genes was assessed by GEPIA Database and Kaplan-Meier Plotter. Results: Results showed that the hub genes, LMNB and CDC20, were up-regulated in breast cancer and indicated worse survival. Therefore, we speculate that these two genes play crucial roles in the process of lung metastasis in breast cancer, and can be used as potential prognostic markers in lung metastasis of breast cancer. Conclusion: Collectively, our study identified two potential key genes in the lung metastasis of breast cancer, which might be applied as the prognostic markers of the precise treatment in breast cancer with lung metastasis.

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