scholarly journals Toxicity and Antigenotoxic Effect of Hispolon Derivatives: Role of Structure in Modulating Cellular Redox State and Thioredoxin Reductase

ACS Omega ◽  
2018 ◽  
Vol 3 (6) ◽  
pp. 5958-5970 ◽  
Author(s):  
Pogakula Chethna ◽  
Shruti S. Iyer ◽  
Vishwa V. Gandhi ◽  
Amit Kunwar ◽  
Beena G. Singh ◽  
...  
Keyword(s):  
Redox State ◽  
Thioredoxin Reductase ◽  
Cellular Redox ◽  
Antigenotoxic Effect ◽  
Cellular Redox State

scholarly journals Cellular Redox State Acts as Switch to Determine the Direction of NNT-Catalyzed Reaction in Cystic Fibrosis Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 967
Author(s):  
Maria Favia ◽  
Anna Atlante
Keyword(s):  
Cystic Fibrosis ◽  
Redox State ◽  
Molecular Mechanisms ◽  
Activity Level ◽  
Cellular Redox ◽  
Redox States ◽  
Mitochondrial Ros ◽  
New Information ◽  
Cellular Dysfunction ◽  
Cellular Redox State

The redox states of NAD and NADP are linked to each other in the mitochondria thanks to the enzyme nicotinamide nucleotide transhydrogenase (NNT) which, by utilizing the mitochondrial membrane potential (mΔΨ), catalyzes the transfer of redox potential between these two coenzymes, reducing one at the expense of the oxidation of the other. In order to define NNT reaction direction in CF cells, NNT activity under different redox states of cell has been investigated. Using spectrophotometric and western blotting techniques, the presence, abundance and activity level of NNT were determined. In parallel, the levels of NADPH and NADH as well as of mitochondrial and cellular ROS were also quantified. CF cells showed a 70% increase in protein expression compared to the Wt sample; however, regarding NNT activity, it was surprisingly lower in CF cells than healthy cells (about 30%). The cellular redox state, together with the low mΔΨ, pushes to drive NNT reverse reaction, at the expense of its antioxidant potential, thus consuming NADPH to support NADH production. At the same time, the reduced NNT activity prevents the NADH, produced by the reaction, from causing an explosion of ROS by the damaged respiratory chain, in accordance with the reduced level of mitochondrial ROS in NNT-loss cells. This new information on cellular bioenergetics represents an important building block for further understanding the molecular mechanisms responsible for cellular dysfunction in cystic fibrosis.

A Novel Redox Based Therapy Targets the Malignant Cellular Redox State

2016 ◽  
Vol 100 ◽  
pp. S119 ◽  
Author(s):  
Dustin Carroll ◽  
Yanming Zhao ◽  
Haining Zhu ◽  
Ines Batinic-Haberle ◽  
Daret St. Clair
Keyword(s):  
Redox State ◽  
Cellular Redox ◽  
Therapy Targets ◽  
Cellular Redox State

Mitochondria and cellular redox state on the route from ageing to Alzheimer’s disease

2020 ◽  
Vol 192 ◽  
pp. 111385 ◽  
Author(s):  
G. Abate ◽  
M. Vezzoli ◽  
M. Sandri ◽  
W. Rungratanawanich ◽  
M. Memo ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State

scholarly journals Phosphorylation of a p38-like MAPK is involved in sensing cellular redox state and drives atypical tubulin polymer assembly in angiosperms

2013 ◽  
Vol 37 (5) ◽  
pp. 1130-1143 ◽  
Author(s):  
PANTELIS LIVANOS ◽  
BASIL GALATIS ◽  
CATHERINE GAITANAKI ◽  
PANAGIOTIS APOSTOLAKOS
Keyword(s):  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State ◽  
Polymer Assembly
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