Antigenotoxic potential of the fermentation broth produced by Paenibacillus polymyxa RNC-D in vitro

Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 μg/ml concentrations induced DNA lesions, whereas the 4 μg/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-α was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-α release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound.

Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

Antigenotoxic effect of melatonin against mercuric chloride in human peripheral blood lymphocytes

Melatonin (MLT) is an extraordinary antioxidant, which plays an important role in reducing reactive oxygen species (ROS) by scavenging them directly or indirectly. Mercury (Hg) is a heavy metal, which induces cytogenetic alterations via various mechanisms, leading to genotoxicity. It induces genotoxicity by enhancing the ROS chiefly. In the present study, the antigenotoxic effect of MLT was evaluated against mercuric chloride (HgCl2). All experiments were conducted in vitro in peripheral blood lymphocytes. Blood cultures were exposed to various concentrations of HgCl2 (2.63, 6.57, and 10.52 microM) for 24 h to study a range of genotoxic parameters. MLT (0.2 mM) supplementation, alone and in combination with the high concentration of Hg, was administered to blood cultures for 24 h. Genotoxic parameters, such as chromosomal aberrations (CAs; structural aberrations (chromatid gaps and breaks, chromosomal gaps and breaks) and numerical aberrations), micronuclei (MNs), and comet assay, were evaluated and analyzed using suitable statistical analysis. Hg treatment revealed a significant increase in CAs, MNs, and comet length. Co-supplementation of MLT along with Hg showed marked protection of these genotoxic end points in treated cultures. In conclusion, our findings suggest that MLT protects against Hg-induced augmentation in genotoxic indices because of its antioxidant property.