Restructuring the Conventional Sugar Beet Industry into a Novel Biorefinery: Fractionation and Bioconversion of Sugar Beet Pulp into Succinic Acid and Value-Added Coproducts

2019 ◽  
Vol 7 (7) ◽  
pp. 6569-6579 ◽  
Author(s):  
Maria Alexandri ◽  
Roland Schneider ◽  
Harris Papapostolou ◽  
Dimitrios Ladakis ◽  
Apostolis Koutinas ◽  
...  
2017 ◽  
Vol 202 ◽  
pp. 415-431 ◽  
Author(s):  
Max Cárdenas-Fernández ◽  
Maria Bawn ◽  
Charlotte Hamley-Bennett ◽  
Penumathsa K. V. Bharat ◽  
Fabiana Subrizi ◽  
...  

Over 8 million tonnes of sugar beet are grown annually in the UK. Sugar beet pulp (SBP) is the main by-product of sugar beet processing which is currently dried and sold as a low value animal feed. SBP is a rich source of carbohydrates, mainly in the form of cellulose and pectin, including d-glucose (Glu), l-arabinose (Ara) and d-galacturonic acid (GalAc). This work describes the technical feasibility of an integrated biorefinery concept for the fractionation of SBP and conversion of these monosaccharides into value-added products. SBP fractionation is initially carried out by steam explosion under mild conditions to yield soluble pectin and insoluble cellulose fractions. The cellulose is readily hydrolysed by cellulases to release Glu that can then be fermented by a commercial yeast strain to produce bioethanol at a high yield. The pectin fraction can be either fully hydrolysed, using physico-chemical methods, or selectively hydrolysed, using cloned arabinases and galacturonases, to yield Ara-rich and GalAc-rich streams. These monomers can be separated using either Centrifugal Partition Chromatography (CPC) or ultrafiltration into streams suitable for subsequent enzymatic upgrading. Building on our previous experience with transketolase (TK) and transaminase (TAm) enzymes, the conversion of Ara and GalAc into higher value products was explored. In particular the conversion of Ara into l-gluco-heptulose (GluHep), that has potential therapeutic applications in hypoglycaemia and cancer, using a mutant TK is described. Preliminary studies with TAm also suggest GluHep can be selectively aminated to the corresponding chiral aminopolyol. The current work is addressing the upgrading of the remaining SBP monomer, GalAc, and the modelling of the biorefinery concept to enable economic and Life Cycle Analysis (LCA).


Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1413
Author(s):  
Seyed Hadi Peighambardoust ◽  
Maryam Jafarzadeh-Moghaddam ◽  
Mirian Pateiro ◽  
José M. Lorenzo ◽  
Rubén Domínguez

The objective of this study was to characterize the properties of pectin extracted from sugar beet pulp using subcritical water (SWE) as compared to conventional extraction (CE). The research involved advanced modeling using response surface methodology and optimization of operational parameters. The optimal conditions for maximum yield of pectin for SWE and CE methods were determined by the central composite design. The optimum conditions of CE were the temperature of 90 °C, time of 240 min, pH of 1, and pectin recovery yield of 20.8%. The optimal SWE conditions were liquid-to-solid (L/S) ratio of 30% (v/w) at temperature of 130 °C for 20 min, which resulted in a comparable yield of 20.7%. The effect of obtained pectins on viscoamylograph pasting and DSC thermal parameters of corn starch was evaluated. The contents of galacturonic acid, degree of methylation, acetylation, and ferulic acid content were higher in the pectin extracted by SWE, while the molecular weight was lower. Similar chemical groups were characterized by FTIR in both SWE and CE pectins. Color attributes of both pectins were similar. Solutions of pectins at lower concentrations displayed nearly Newtonian behavior. The addition of both pectins to corn starch decreased pasting and DSC gelatinization parameters, but increased ΔH. The results offered a promising scalable approach to convert the beet waste to pectin as a value-added product using SWE with improved pectin properties.


2021 ◽  
Vol 7 (3) ◽  
pp. 215
Author(s):  
Luís Martins ◽  
Margarida Palma ◽  
Angel Angelov ◽  
Elke Nevoigt ◽  
Wolfgang Liebl ◽  
...  

Agro-industrial residues are low-cost carbon sources (C-sources) for microbial growth and production of value-added bioproducts. Among the agro-industrial residues available, those rich in pectin are generated in high amounts worldwide from the sugar industry or the industrial processing of fruits and vegetables. Sugar beet pulp (SBP) hydrolysates contain predominantly the neutral sugars d-glucose, l-arabinose and d-galactose, and the acidic sugar d-galacturonic acid. Acetic acid is also present at significant concentrations since the d-galacturonic acid residues are acetylated. In this study, we have examined and optimized the performance of a Rhodotorula mucilaginosa strain, isolated from SBP and identified at the molecular level during this work. This study was extended to another oleaginous red yeast species, R. toruloides, envisaging the full utilization of the C-sources from SBP hydrolysate (at pH 5.0). The dual role of acetic acid as a carbon and energy source and as a growth and metabolism inhibitor was examined. Acetic acid prevented the catabolism of d-galacturonic acid and l-arabinose after the complete use of the other C-sources. However, d-glucose and acetic acid were simultaneously and efficiently metabolized, followed by d-galactose. SBP hydrolysate supplementation with amino acids was crucial to allow d-galacturonic acid and l-arabinose catabolism. SBP valorization through the production of lipids and carotenoids by Rhodotorula strains, supported by complete catabolism of the major C-sources present, looks promising for industrial implementation.


2021 ◽  
pp. 126580
Author(s):  
Zeba Usmani ◽  
Minaxi Sharma ◽  
Deepti Diwan ◽  
Manikant Tripathi ◽  
Eric Whale ◽  
...  

Author(s):  
S.V. Meshcheryakov ◽  
◽  
I.S. Eremin ◽  
D.O. Sidorenko ◽  
M.S. Kotelev ◽  
...  
Keyword(s):  

2016 ◽  
pp. 565-570
Author(s):  
Huang Qin ◽  
Zhu Si-ming ◽  
Zeng Di ◽  
Yu Shu-juan

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.


2012 ◽  
pp. 756-761 ◽  
Author(s):  
Miroslav Hutnan ◽  
Štefan Tóth ◽  
Igor Bodík ◽  
Nina Kolesárová ◽  
Michal Lazor ◽  
...  

The possibility of joint treatment of spent sugar beet pulp and wastewater from a sugar factory was studied in this work. Works focused on processing of spent sugar beet pulp separately or together with other substrates can be found in the literature. In the case of some sugar factories, which have spare capacity in the anaerobic reactor on an anaerobic-aerobic wastewater treatment plant, joint processing of spent sugar beet pulp and wastewater from the sugar factory might be an interesting option. The results of the operation of a pilot plant of an anaerobic reactor with a capacity of 3.5 m3 are discussed. Operation of the pilot plant confirmed the possibility of cofermentation of these materials. The organic loading rate achieved in the anaerobic reactor was higher than 6 kg/(m3·d) (COD), while more than half of the load was provided by spent sugar beet pulp. The addition of sugar beet pulp decreased the concentration of ammonia nitrogen in the anaerobic reactor and it was even necessary to add nitrogen. However, the nitrogen content in sludge water depends on the C:N ratio in the processed sugar beet pulp, therefore this knowledge cannot be generalized. About 1.5 to 2-fold biogas production can be expected from the cofermentation of wastewater with sugar beet pulp in an anaerobic reactor, compared with the biogas production from just wastewater treatment.


2015 ◽  
Vol 6 (6) ◽  
pp. 351-378
Author(s):  
Hend Aziz ◽  
A. Kholif
Keyword(s):  

Biofuels ◽  
2021 ◽  
pp. 1-8
Author(s):  
Saida Ibragić ◽  
Narcisa Smječanin ◽  
Ranko Milušić ◽  
Mirza Nuhanović

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