Magnetic Fe@Y Composites as Efficient Recoverable Catalysts for the Valorization of the Recalcitrant Marine Sulfated Polysaccharide Ulvan

Background: 1,2,3-triazoles are an important class of organic compounds and because of their aromatic stability, they are not easily reduced, oxidized or hydrolyzed in acidic and basic environments. Moreover, 1,2,3-triazole derivatives are known by their important biological activities and have drawn considerable attention due to their variety of properties. The synthesis of this nucleus, based on the click chemistry concept, through the 1,3-dipolar addition reaction between azides and alkynes is a well-known procedure. This reaction has a wide range of applications, especially on the development of new drugs. Methods: The most prominent eco-friendly methods for the synthesis of triazoles under microwave irradiation published in articles from 2012-2018 were reviewed. Results: In this review, we cover some of the recent eco-friendly CuAAC procedures for the click synthesis of 1,2,3-triazoles with remarks to new and easily recoverable catalysts, such as rhizobial cyclic β-1,2 glucan; WEB (water extract of banana); biosourced cyclosophoraose (CyS); egg shell powder (ESP); cyclodextrin (β- CD); fish bone powder; nanoparticle-based catalyst, among others. Conclusion: These eco-friendly procedures are a useful tool for the synthesis of 1,2,3-triazoles, providing many advantages on the synthesis of this class, such as shorter reaction times, easier work-up and higher yields when compared to classical procedures. Moreover, these methodologies can be applied to the industrial synthesis of drugs and to other areas.

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Anti-allergy Effect of Sulfated Polysaccharide from Sargassum polycystum on Dinitrofluorobenzene Induced Allergic Contact Dermatitis in Mice

The Natural Products Journal ◽

10.2174/2210315510999200714111931 ◽

2020 ◽

Vol 10 ◽

Author(s):

Sharmaine Y. Dela Cruz ◽

Ross D. Vasquez ◽

Reginald B. Salonga ◽

Mary Jho-Anne T. Corpuz

Keyword(s):

Contact Dermatitis ◽

Allergic Contact Dermatitis ◽

Sulfated Polysaccharide ◽

Hot Water ◽

Thickness Gauge ◽

Sargassum Polycystum ◽

Acd Model ◽

Allergic Contact ◽

Ventral Skin ◽

Allergic Effect

Background: Sargassum polycystum C. Agardh has potent antioxidant and anti-inflammatory properties. However, its anti-allergic effect has not yet been reported. In this study, we investigated the anti-allergic effects of sulfated polysaccharide of S. polycystum (SPSP) in Dinitrofluorobenzene (DNFB)- induced allergic contact dermatitis animal model. Methods: SPSP was extracted through hot water extraction method and was subjected to compositional analyses. For the allergic contact dermatitis (ACD) model, symptoms were induced by the topical application of 0.5% DNFB on the shaved ventral skin of mice. SPSP (500, 1000, and 2000 mg/kg) and Prednisolone were orally administered for seven days after sensitization. Elicitation was performed seven days later with 0.2% DNFB. After this, ear thickness was measured at baseline and 24 hours post elicitation using a dial thickness gauge. Serum of mice was obtained 24 hours post elicitation, and the level of IFNγ and TNF Results: SPSP afforded 33.6% carbohydrates, 23.7% sulfate, 7.5% protein, and 1.5% uronic acid contents. SPSP inhibited the ear swelling and cytokines (IFNγ and TNF Conclusion: These findings showed that the sulfated polysaccharide from S. polycytum is a potential natural source to treat Allergic Contact Dermatitis. The effect is attributed to polysaccharide-protein complex present in the extract, but further studies are needed to establish the exact mechanism of action of SPSP in the treatment of the disease.

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Investigation of the biological functions of heparan sulfate using a chemoenzymatic synthetic approach

RSC Chemical Biology ◽

10.1039/d0cb00199f ◽

2021 ◽

Author(s):

Zhangjie Wang ◽

Katelyn Arnold ◽

Vijay Manohar Dhurandhare ◽

Yongmei Xu ◽

Jian Liu

Keyword(s):

Heparan Sulfate ◽

Sulfated Polysaccharide ◽

Synthetic Approach ◽

Biological Functions ◽

Animal Kingdom

Heparan sulfate (HS) is a highly sulfated polysaccharide playing essential physiological and pathophysiological roles in the animal kingdom.

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Suppressive effects of sulfated polysaccharide ascophyllan isolated from Ascophyllum nodosum on the production of NO and ROS in LPS-stimulated RAW264.7 cells

Bioscience Biotechnology and Biochemistry ◽

10.1093/bbb/zbaa115 ◽

2020 ◽

Vol 85 (4) ◽

pp. 882-889

Author(s):

Yan Liang ◽

Shijiao Zha ◽

Masanobu Tentaku ◽

Takasi Okimura ◽

Zedong Jiang ◽

...

Keyword(s):

Nitric Oxide ◽

Ascophyllum Nodosum ◽

Sulfated Polysaccharide ◽

Intracellular Reactive Oxygen Species ◽

Raw264.7 Cells ◽

Dependent Manner ◽

Oxygen Species ◽

Oxide Synthase ◽

Dose Dependent ◽

Inducible Nitric Oxide

ABSTRACT In this study, we found that a sulfated polysaccharide isolated from the brown alga Ascophyllum nodosum, ascophyllan, showed suppressive effects on stimulated RAW264.7 cells. Ascophyllan significantly inhibited expression of inducible nitric oxide synthase mRNA and excessive production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells in a dose-dependent manner without affecting the viability of RAW264.7 cells. Ascophyllan also reduced the elevated level of intracellular reactive oxygen species (ROS) in LPS-stimulated RAW264.7 cells. Furthermore, preincubation with ascophyllan resulted in concentration-dependent decrease in ROS production in phorbol 12-myristate-13-acetate-stimulated RAW264.7 cells. Our results suggest that ascophyllan can exhibit anti-inflammatory effects on stimulated macrophages mainly through the attenuation of NO and ROS productions.

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