Rapid and Quantitative Detection of Prostate Specific Antigen with a Quantum Dot Nanobeads-Based Immunochromatography Test Strip

2014 ◽  
Vol 6 (9) ◽  
pp. 6406-6414 ◽  
Author(s):  
Xue Li ◽  
Wenbin Li ◽  
Qiuhua Yang ◽  
Xiaoqun Gong ◽  
Weisheng Guo ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Test Strip ◽  
Quantitative Detection

scholarly journals Quantitative Reverse Transcription-PCR Assay with an Internal Standard for the Detection of Prostate-specific Antigen mRNA

1999 ◽  
Vol 45 (9) ◽  
pp. 1397-1407 ◽  
Author(s):  
Alice Ylikoski ◽  
Minna Sjöroos ◽  
Åke Lundwall ◽  
Matti Karp ◽  
Timo Lövgren ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Detection Limit ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Internal Standard ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

Abstract Background: Circulating prostate cells can be detected with a reverse transcription-PCR (RT-PCR) assay for prostate-specific antigen (PSA) mRNA. We have developed a new quantitative RT-PCR method for measuring PSA mRNA. Methods: The method uses a PSA-like internal standard (IS) mRNA that is added into the sample at the beginning of the RNA extraction and coamplified by RT-PCR with the PSA in the sample. After PCR amplification, the IS and PSA products are selectively detected by hybridization in a microtitration plate using probes labeled with fluorescent europium chelates. Results: The method was validated with PSA and IS mRNAs and PSA-expressing cells to obtain a detection limit of 50 PSA mRNA copies (i.e., signal 2 times the mean of zero signal), linearity up to 106 copies, and detection of a single PSA-expressing cell. In preliminary evaluations, 60% (n = 10) of the prostate cancer patients with skeletal metastases gave results above the detection limit (500 PSA mRNA copies in 5 mL of blood). The total number of PSA copies ranged from 900 ± 200 to 44 100 ± 4900 (mean ± SD) in the samples, corresponding to ∼1–100 PSA-expressing cells in 5 mL of blood. In the controls (n = 34), none of the healthy females and 2 of 19 healthy males had detectable PSA mRNA [700 ± 100 and 2000 ± 900 (mean ± SD) PSA mRNA copies in 5 mL of blood for the 2 males]. Conclusions: The assay provides sensitive and quantitative detection of PSA mRNA expression from blood samples and can be used to establish the clinically significant number of PSA mRNA copies in prostate cancer.

scholarly journals Molybdenum disulfide quantum dot based highly sensitive impedimetric immunoassay for prostate specific antigen

2017 ◽  
Vol 184 (12) ◽  
pp. 4647-4654 ◽  
Author(s):  
Manil Kukkar ◽  
Suman Singh ◽  
Nishant Kumar ◽  
Satish K. Tuteja ◽  
Ki-Hyun Kim ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Prostate Specific Antigen ◽  
Molybdenum Disulfide ◽  
Specific Antigen ◽  
Highly Sensitive

Lateral Flow Immunoassay Based on Gold Magnetic Nanoparticles for the Protein Quantitative Detection: Prostate-Specific Antigen

2021 ◽  
pp. 114265
Author(s):  
Yu Cai ◽  
Shanshan Zhang ◽  
Chen Dong ◽  
Jiangcun Yang ◽  
Ting Ma ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection

scholarly journals Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen

Sensors ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 197 ◽  
Author(s):  
Shashi Bhuckory ◽  
Olivier Lefebvre ◽  
Xue Qiu ◽  
Karl Wegner ◽  
Niko Hildebrandt
Keyword(s):  
Quantum Dot ◽  
Prostate Specific Antigen ◽  
Specific Antigen

A new visual immunoassay for prostate-specific antigen using near-infrared excited CuxS nanocrystals and imaging on a smartphone

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3716-3720 ◽  
Author(s):  
Shuzhen Lv ◽  
Kangyao Zhang ◽  
Dianping Tang
Keyword(s):  
Prostate Specific Antigen ◽  
Near Infrared ◽  
Specific Antigen ◽  
Cancer Biomarkers ◽  
Quantitative Detection ◽  
Thermal Imager ◽  
Infrared Thermal Imager

A photothermal immune-imaging assay was innovatively designed for the visual quantitative detection of cancer biomarkers by coupling CuxS nanocrystals with a portable infrared thermal imager on a smartphone.

scholarly journals Au–Ag assembled on silica nanoprobes for visual semiquantitative detection of prostate-specific antigen

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hyung-Mo Kim ◽  
Jaehi Kim ◽  
Jaehyun An ◽  
Sungje Bock ◽  
Xuan-Hung Pham ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Early Diagnosis ◽  
Prostate Specific Antigen ◽  
Test Line ◽  
Specific Antigen ◽  
Test Strip ◽  
Clinical Samples ◽  
Color Intensity ◽  
Ag Nps ◽  
Relative Standard

Abstract Background Blood prostate-specific antigen (PSA) levels are widely used as diagnostic biomarkers for prostate cancer. Lateral-flow immunoassay (LFIA)-based PSA detection can overcome the limitations associated with other methods. LFIAbased PSA detection in clinical samples enables prognosis and early diagnosis owing to the use of high-performance signal reporters. Results Here, a semiquantitative LFIA platform for PSA detection in blood was developed using Au–Ag nanoparticles (NPs) assembled on silica NPs (SiO2@Au–Ag NPs) that served as signal reporters. Synthesized SiO2@Au–Ag NPs exhibited a high absorbance at a wide wavelength range (400–800 nm), with a high scattering on nitrocellulose membrane test strips. In LFIA, the color intensity of the test line on the test strip differed depending on the PSA concentration (0.30–10.00 ng/mL), and bands for the test line on the test strip could be used as a standard. When clinical samples were assessed using this LFIA, a visual test line with particular color intensity observed on the test strip enabled the early diagnosis and prognosis of patients with prostate cancer based on PSA detection. In addition, the relative standard deviation of reproducibility was 1.41%, indicating high reproducibility, and the signal reporter showed good stability for 10 days. Conclusion These characteristics of the signal reporter demonstrated the reliability of the LFIA platform for PSA detection, suggesting potential applications in clinical sample analysis.

CdTe@SiO2 signal reporters-based fluorescent immunosensor for quantitative detection of prostate specific antigen

2019 ◽  
Author(s):  
Yuting Zhao ◽  
Wen Gao ◽  
Xiaoxiao Ge ◽  
Suiqiong Li ◽  
Dan Du ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Quantitative Detection

Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopy

Talanta ◽  
2007 ◽  
Vol 71 (4) ◽  
pp. 1494-1499 ◽  
Author(s):  
Kagan Kerman ◽  
Tatsuro Endo ◽  
Masatoshi Tsukamoto ◽  
Miyuki Chikae ◽  
Yuzuru Takamura ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Fluorescence Microscopy ◽  
Prostate Specific Antigen ◽  
Specific Antigen
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