Yeast and horse liver alcohol dehydrogenases: potential problems in target size analysis and evidence for a monomer active unit

Biochemistry ◽  
1987 ◽  
Vol 26 (12) ◽  
pp. 3340-3347 ◽  
Author(s):  
M. D. Suarez ◽  
S. Ferguson-Miller
Biochemistry ◽  
1986 ◽  
Vol 25 (14) ◽  
pp. 4051-4057 ◽  
Author(s):  
Michael J. Seagar ◽  
Catherine Labbe-Jullie ◽  
Claude Granier ◽  
Alexandra Goll ◽  
Hartmut Glossmann ◽  
...  

1990 ◽  
Vol 1051 (3) ◽  
pp. 306-309 ◽  
Author(s):  
Shuichi Saheki ◽  
Takayoshi Kuno ◽  
Chikako Tanaka ◽  
Nozomu Takeuchi ◽  
Ferid Murad

Zygote ◽  
1995 ◽  
Vol 3 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Akira Ushiyama ◽  
Kazuyoshi Chiba ◽  
Akihiro Shima ◽  
Motonori Hoshi

SummaryIn the starfish Asterias amurensis, the jelly coat of the eggs contains a glycoprotein essential for the induction of the acrosome reaction in homologous spermatozoa that is termed the acrosome-reaction-inducing substance (ARIS).ARIS is a highly sulphated and fucose-rich glycoprotein of extremely high molecular mass(>104 kDa). ARIS was irradiated with high-energy eletrons in order to estimate the minimum size required for its biological activity. The minimum functional unit or target size of ARIS was estimated to be c. 14 kDa by target size analysis. ARIS was significantly disintegrated by the irradiation, yet the total sugar content was not apparently reduced. The binding of 125I-labelled ARIS to spermatozoa competed with that of irradiated ARIS, although the affinity of ARIS was much reduced after irradiation.


1983 ◽  
Vol 2 (10) ◽  
pp. 1729-1732 ◽  
Author(s):  
D.R. Ferry ◽  
A. Goll ◽  
H. Glossmann

2004 ◽  
Vol 03 (06) ◽  
pp. 757-763 ◽  
Author(s):  
PAMELA WHITFIELD ◽  
LYNDON MITCHELL

Powder X-ray diffraction has become a cornerstone technique for deriving crystallite size in nanoscience due to speed and "simplicity". Unfortunately, this apparently simple technique commonly has unexpected problems. Anisotropic peak broadening related to crystallite shape, defects, and microstrain occurs frequently in nanomaterials and can significantly complicate the analysis. In some instances, the usage of the conventional single peak approach would give erroneous results, and in others, this type of analysis is not even possible. A number of different nanocrystalline oxides have been examined to determine their crystallite sizes by different techniques. They differ in terms of crystal symmetry, crystallinity, density, and present different challenges with regard to size analysis.


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