High-affinity transport of L-glutamine by a plasma membrane preparation from rat brain

Biochemistry ◽  
1989 ◽  
Vol 28 (20) ◽  
pp. 8083-8087 ◽  
Author(s):  
Robert J. Roon ◽  
Sharon A. Shofner ◽  
James F. Koerner
Keyword(s):  
Plasma Membrane ◽  
Rat Brain ◽  
Membrane Preparation ◽  
High Affinity ◽  
Plasma Membrane Preparation

scholarly journals Biotransformation of endorphins by a synaptosomal plasma membrane preparation of rat brain and by human serum

1979 ◽  
Vol 86 (4) ◽  
pp. 1296-1303 ◽  
Author(s):  
J.P.H. Burbach ◽  
J.G. Loeber ◽  
J. Verhoef ◽  
E.R. de Kloet ◽  
D. de Wied
Keyword(s):  
Plasma Membrane ◽  
Human Serum ◽  
Rat Brain ◽  
Membrane Preparation ◽  
Synaptosomal Plasma Membrane ◽  
Plasma Membrane Preparation

scholarly journals A rapid immunological procedure for the isolation of hormonally sensitive rat fat-cell plasma membrane

1976 ◽  
Vol 154 (1) ◽  
pp. 11-21 ◽  
Author(s):  
J P Luzio ◽  
A C Newby ◽  
C N Hales
Keyword(s):  
Plasma Membrane ◽  
Adenylate Cyclase ◽  
Plasma Membranes ◽  
Membrane Preparation ◽  
Fat Cells ◽  
Fat Cell ◽  
Cell Plasma ◽  
Rat Fat Cells ◽  
Plasma Membrane Preparation ◽  
Isolated Rat

1. A rapid method for the isolation of hormonally sensitive rat fat-cell plasma membranes was developed by using immunological techniques. 2. Rabbit anti-(rat erythrocyte) sera were raised and shown to cross-react with isolated rat fat-cells. 3. Isolated rat fat-cells were coated with rabbit anti-(rat erythrocyte) antibodies, homogenized and the homogenate made to react with an immunoadsorbent prepared by covalently coupling donkey anti-(rabbit globulin) antibodies to aminocellulose. Uptake of plasma membrane on to the immunoadsorbent was monitored by assaying the enzymes adenylate cyclase and 5′-nucleotidase and an immunological marker consisting of a 125I-labelled anti-(immunoglobulin G)-anti-cell antibody complex bound to the cells before fractionation. Contamination of the plasma-membrane preparation by other subcellular fractions was also investigated. 4. By using this technique, a method was developed allowing 25-40% recovery of plasma membrane from fat-cell homogenates within 30 min of homogenization. 5. Adenylate cyclase in the isolated plasma-membrane preparation was stimulated by 5 μm-adrenaline.

Steroid sequestration within the rat adrenal zona glomerulosa

1988 ◽  
Vol 117 (2) ◽  
pp. 191-NP ◽  
Author(s):  
S. M. Laird ◽  
G. P. Vinson ◽  
B. J. Whitehouse
Keyword(s):  
Plasma Membrane ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Zona Glomerulosa ◽  
Membrane Preparation ◽  
The Media ◽  
Considerable Concentration ◽  
Highly Correlated ◽  
Plasma Membrane Preparation

ABSTRACT Accumulated data from in-vitro experiments have suggested that 18-hydroxysteroids may be stored within the intact rat adrenal zona glomerulosa. The phenomenon was further investigated by comparing the amount of steroid remaining in the zona glomerulosa tissue with that secreted into the media during incubation in vitro. The results showed that 18-hydroxydeoxycorticosterone (18-OH-DOC) and 18-hydroxycorticosterone (18-OH-B) were retained within the tissue against a considerable concentration gradient, with smaller amounts of aldosterone and corticosterone. Lysis of the intact zona glomerulosa, by preincubation in distilled water, yielded an enriched plasma membrane preparation. After subsequent incubation in Krebs–Ringer bicarbonate this preparation contained significantly more 18-OH-DOC than did the intact tissue, suggesting that tissuesequestered 18-OH-DOC is normally metabolized to other products. These may include 18-OH-B and aldosterone. Fractionation of homogenized intact zona glomerulosa and the enriched plasma membrane preparation by density gradient centrifugation showed that tissue 18-OH-DOC banded in fractions of density 1·063– 1·21 g/ml and that its distribution was highly correlated with protein. Corticosterone, 18-OH-B and aldosterone banded like added free [3H]18-OH-DOC in fractions of density < 1·006 g/ml. The results suggest that 18-OH-DOC is the major sequestered steroid within the rat adrenal zona glomerulosa and that this sequestration is attributable to the association of 18-OH-DOC with a high-density component of the plasma membrane. J. Endocr. (1988) 117, 191–196

Interaction of phospholipase A with an axon plasma membrane preparation

Life Sciences ◽  
1976 ◽  
Vol 18 (7) ◽  
pp. 751-758 ◽  
Author(s):  
Jeffrey L. Denburg
Keyword(s):  
Plasma Membrane ◽  
Phospholipase A ◽  
Membrane Preparation ◽  
Plasma Membrane Preparation

scholarly journals Plasma Membrane Preparation from Lilium davidii and Oryza sativa Mature and Germinated Pollen

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (10) ◽  
Author(s):  
Ning Yang ◽  
Bing Han ◽  
Lingtong Liu ◽  
Hao Yang ◽  
Tai Wang
Keyword(s):  
Oryza Sativa ◽  
Plasma Membrane ◽  
Membrane Preparation ◽  
Germinated Pollen ◽  
Plasma Membrane Preparation ◽  
Lilium Davidii

scholarly journals GTPase Activity in Rice Plasma Membrane Preparation Enhanced by a Priming Effector for Plant Defence Reactions

1995 ◽  
Vol 20 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Yasuharu SEKIZAWA ◽  
Hideyuki AOYAMA ◽  
Makoto KIMURA ◽  
Isamu YAMAGUCHI
Keyword(s):  
Plasma Membrane ◽  
Plant Defence ◽  
Membrane Preparation ◽  
Gtpase Activity ◽  
Defence Reactions ◽  
Plasma Membrane Preparation
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