scholarly journals Identification of the Copper(II) Coordinating Residues in the Prion Protein by Metal-Catalyzed Oxidation Mass Spectrometry: Evidence for Multiple Isomers at Low Copper(II) Loadings†

Biochemistry ◽  
2008 ◽  
Vol 47 (35) ◽  
pp. 9258-9268 ◽  
Author(s):  
Rapole Srikanth ◽  
Jonathan Wilson ◽  
Colin S. Burns ◽  
Richard W. Vachet
Keyword(s):  
Mass Spectrometry ◽  
Prion Protein ◽  
Metal Catalyzed Oxidation ◽  
Metal Catalyzed ◽  
Catalyzed Oxidation

Transition Metal−Peptide Binding Studied by Metal-Catalyzed Oxidation Reactions and Mass Spectrometry

2006 ◽  
Vol 78 (7) ◽  
pp. 2432-2438 ◽  
Author(s):  
Juma D. Bridgewater ◽  
Jihyeon Lim ◽  
Richard W. Vachet
Keyword(s):  
Mass Spectrometry ◽  
Transition Metal ◽  
Peptide Binding ◽  
Oxidation Reactions ◽  
Metal Catalyzed Oxidation ◽  
Metal Catalyzed ◽  
Catalyzed Oxidation

Ambivalent role of ascorbic acid in the metal-catalyzed oxidation of oligopeptides

2021 ◽  
pp. 111510
Author(s):  
Nikolett Bodnár ◽  
Katalin Várnagy ◽  
Lajos Nagy ◽  
Gizella Csire ◽  
Csilla Kállay
Keyword(s):  
Ascorbic Acid ◽  
Metal Catalyzed Oxidation ◽  
Metal Catalyzed ◽  
Catalyzed Oxidation

scholarly journals Metal-catalyzed oxidation renders silver intensification selective. Applications for the histochemistry of diaminobenzidine and neurofibrillary changes.

1986 ◽  
Vol 34 (12) ◽  
pp. 1667-1672 ◽  
Author(s):  
F Gallyas ◽  
J R Wolff
Keyword(s):  
Hydrogen Peroxide ◽  
Oxidative Polymerization ◽  
Silver Coating ◽  
Neurofibrillary Changes ◽  
Iodide Ions ◽  
Tissue Sections ◽  
Metal Catalyzed Oxidation ◽  
Pre Treatment ◽  
Metal Catalyzed ◽  
Catalyzed Oxidation

Physical developers can increase the visibility of end products of certain histochemical reactions, such as oxidative polymerization of diaminobenzidine and selective binding of complex silver iodide ions to Alzheimer's neurofibrillary changes. Unfortunately, this intensification by silver coating is generally superimposed on a nonspecific staining originating from the argyrophil III reaction, which also takes place when tissue sections are treated with physical developers. The present study reveals that the argyrophil III reaction can be suppressed when tissue sections are treated with certain metal ions and hydrogen peroxide before they are transferred to the physical developer. The selective intensification of Alzheimer's neurofibrillary changes requires a pre-treatment with lanthanum nitrate (10 mM/liter) and 3% hydrogen peroxide for 1 hr. The diaminobenzidine reaction can be selectively intensified when physical development is preceded by consecutive treatments with copper sulfate (10 mM/liter, pH 5, 10 min) and hydrogen peroxide (3%, pH 7, 10 min). In peroxidase histochemistry, this high-grade intensification may help to increase specificity and reduce the threshold of detectability in tracing neurons with horseradish peroxidase or in immunohistochemistry when the peroxidase-antiperoxidase method is used.

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