Immobilized Proteoliposome Affinity Chromatography for Quantitative Analysis of Specific Interactions between Solutes and Membrane Proteins. Interaction of Cytochalasin B andd-Glucose with the Glucose Transporter Glut1

Biochemistry ◽  
1996 ◽  
Vol 35 (33) ◽  
pp. 11012-11012
Author(s):  
Qing Yang ◽  
Per Lundahl
1992 ◽  
Vol 283 (3) ◽  
pp. 643-646 ◽  
Author(s):  
C K Yi ◽  
B M Charalambous ◽  
V C Emery ◽  
S A Baldwin

The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain greater than 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent Mr, probably as a result of glycosylation differences.


1997 ◽  
Vol 776 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Lili Lu ◽  
Andreas Lundqvist ◽  
Cheng-Ming Zeng ◽  
Christine Lagerquist ◽  
Per Lundahl

2001 ◽  
Vol 59 (3) ◽  
pp. 985-989 ◽  
Author(s):  
Andrea D. Hodgkinson ◽  
Beverley A. Millward ◽  
Andrew G. Demaine

Endocrinology ◽  
1992 ◽  
Vol 131 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Y Hosaka ◽  
M Tawata ◽  
A Kurihara ◽  
M Ohtaka ◽  
T Endo ◽  
...  

Brain ◽  
2008 ◽  
Vol 131 (7) ◽  
pp. 1831-1844 ◽  
Author(s):  
A. Suls ◽  
P. Dedeken ◽  
K. Goffin ◽  
H. Van Esch ◽  
P. Dupont ◽  
...  

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