Sequence Context Profoundly Influences the Mutagenic Potency of Trans-Opened Benzo[a]pyrene 7,8-Diol 9,10-Epoxide−Purine Nucleoside Adducts in Site-Specific Mutation Studies†

Biochemistry ◽  
1998 ◽  
Vol 37 (25) ◽  
pp. 9127-9137 ◽  
Author(s):  
John E. Page ◽  
Barbara Zajc ◽  
Toshinari Oh-hara ◽  
Mahesh K. Lakshman ◽  
Jane M. Sayer ◽  
...  
Biochemistry ◽  
1999 ◽  
Vol 38 (3) ◽  
pp. 1144-1152 ◽  
Author(s):  
Ingrid Pontén ◽  
Jane M. Sayer ◽  
Anthony S. Pilcher ◽  
Haruhiko Yagi ◽  
Subodh Kumar ◽  
...  

2002 ◽  
Vol 70 (2) ◽  
pp. 787-793 ◽  
Author(s):  
Patricia Guerry ◽  
Christine M. Szymanski ◽  
Martina M. Prendergast ◽  
Thomas E. Hickey ◽  
Cheryl P. Ewing ◽  
...  

ABSTRACT The outer cores of the lipooligosaccharides (LOS) of many strains of Campylobacter jejuni mimic human gangliosides in structure. A population of cells of C. jejuni strain 81-176 produced a mixture of LOS cores which consisted primarily of structures mimicking GM2 and GM3 gangliosides, with minor amounts of structures mimicking GD1b and GD2. Genetic analyses of genes involved in the biosynthesis of the outer core of C. jejuni 81-176 revealed the presence of a homopolymeric tract of G residues within a gene encoding CgtA, an N-acetylgalactosaminyltransferase. Variation in the number of G residues within cgtA affected the length of the open reading frame, and these changes in cgtA corresponded to a change in LOS structure from GM2 to GM3 ganglioside mimicry. Site-specific mutation of cgtA in 81-176 resulted in a major LOS core structure that lacked GalNAc and resembled GM3 ganglioside. Compared to wild-type 81-176, the cgtA mutant showed a significant increase in invasion of INT407 cells. In comparison, a site-specific mutation of the neuC1 gene resulted in the loss of sialic acid in the LOS core and reduced resistance to normal human serum but had no affect on invasion of INT407 cells.


PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e60216 ◽  
Author(s):  
Benjamin Davies ◽  
Graham Davies ◽  
Christopher Preece ◽  
Rathi Puliyadi ◽  
Dorota Szumska ◽  
...  

1998 ◽  
Vol 864 (1 ENZYME ENGINE) ◽  
pp. 561-564 ◽  
Author(s):  
SZU-PEI WU ◽  
CHICHI LIU ◽  
TZANN-SHUN HWANG ◽  
JIN-TANN CHEN ◽  
HSIN TSAI

2008 ◽  
Vol 37 (1) ◽  
pp. 94-95 ◽  
Author(s):  
Takashi Matsumura ◽  
Masayuki Ogino ◽  
Kango Nagayoshi ◽  
Kenzo Fujimoto

Genetics ◽  
2008 ◽  
Vol 180 (3) ◽  
pp. 1511-1524 ◽  
Author(s):  
Saharon Rosset ◽  
R. Spencer Wells ◽  
David F. Soria-Hernanz ◽  
Chris Tyler-Smith ◽  
Ajay K. Royyuru ◽  
...  

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