Electrostatic Interaction and Complex Formation between Gum Arabic and Bovine Serum Albumin

2010 ◽  
Vol 11 (12) ◽  
pp. 3367-3374 ◽  
Author(s):  
T. Vinayahan ◽  
P. A. Williams ◽  
G. O. Phillips
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Electrostatic Interaction ◽  
Bovine Serum ◽  
Gum Arabic

scholarly journals Formation of Nanocomplexes between Carboxymethyl Inulin and Bovine Serum Albumin via pH-Induced Electrostatic Interaction

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3056 ◽  
Author(s):  
Guiying Huang ◽  
Jun Liu ◽  
Weiping Jin ◽  
Zihao Wei ◽  
Chi-Tang Ho ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Electrostatic Interaction ◽  
Bovine Serum ◽  
Titration Calorimetry ◽  
Food Ingredients ◽  
Enthalpy Changes ◽  
Ft Ir

As a functional polysaccharide, inulin was carboxymethylated and it formed nanocomplexes with bovine serum albumin (BSA). The success of obtaining carboxymethyl inulin (CMI) was confirmed by a combination of Fourier transform Infrared (FT-IR), Raman spectroscopy, gel permeation chromatography (GPC), and titration. The effects of pH and ionic strength on the formation of CMI/BSA nanocomplexes were investigated. Our results showed that the formation of complex coacervate (pHφ1) and dissolution of CMI/BSA insoluble complexes (pHφ2) appeared in pH near 4.85 and 2.00 respectively. FT-IR and Raman data confirmed the existence of electrostatic interaction and hydrogen bonding between CMI and BSA. The isothermal titration calorimetry (ITC) results suggested that the process of complex formation was spontaneous and exothermic. The complexation was dominated by enthalpy changes (∆Η < 0, ∆S < 0) at pH 4.00, while it was contributed by enthalpic and entropic changes (∆Η < 0, ∆S > 0) at pH 2.60. Irregularly shaped insoluble complexes and globular soluble nanocomplexes (about 150 nm) were observed in CMI/BSA complexes at pH 4.00 and 2.60 while using optical microscopy and atomic force microscopy, respectively. The sodium chloride suppression effect on CMI/BSA complexes was confirmed by the decrease of incipient pH for soluble complex formation (or pHc) and pHφ1 under different sodium chloride concentrations. This research presents a new functional system with the potential for delivering bioactive food ingredients.

Heteroprotein complex formation of bovine serum albumin and lysozyme: Structure and thermal stability

2018 ◽  
Vol 74 ◽  
pp. 267-274 ◽  
Author(s):  
Monique Barreto Santos ◽  
Carlos Wanderlei Piler de Carvalho ◽  
Edwin Elard Garcia-Rojas
Keyword(s):  
Thermal Stability ◽  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Bovine Serum

scholarly journals INTERMOLECULAR COMPLEX FORMATION BETWEEN METHYL ORANGE AND BOVINE SERUM ALBUMIN ON THE SURFACE OF HYDROXYAPATITE IN AN AQUEOUS PHASE

2005 ◽  
Vol 18 (0) ◽  
pp. 65-74 ◽  
Author(s):  
SABURO SHIMABAYASHI ◽  
MAKIKO FUKUOKA ◽  
TOMOAKI HINO
Keyword(s):  
Methyl Orange ◽  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Aqueous Phase ◽  
Bovine Serum ◽  
Intermolecular Complex

scholarly journals Stopped-Flow Kinetic Study of the Complex Formation between Pyridoxal 5′-Phosphate and Bovine Serum Albumin

1986 ◽  
Vol 59 (11) ◽  
pp. 3399-3403 ◽  
Author(s):  
Kiyofumi Murakami ◽  
Yukio Kubota ◽  
Yasuo Fujisaki ◽  
Takayuki Sano
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Kinetic Study ◽  
Bovine Serum ◽  
Stopped Flow

Complex Formation of Bovine Serum Albumin with a Poly(ethylene glycol) Lipid Conjugate

2007 ◽  
Vol 8 (7) ◽  
pp. 2244-2249 ◽  
Author(s):  
Valeria Castelletto ◽  
Marta Krysmann ◽  
Antonios Kelarakis ◽  
Paula Jauregi
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Ethylene Glycol ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Lipid Conjugate

Chitosan–bovine serum albumin complex formation: A model to design an enzyme isolation method by polyelectrolyte precipitation

2010 ◽  
Vol 878 (19) ◽  
pp. 1543-1548 ◽  
Author(s):  
Valeria Boeris ◽  
Beatriz Farruggia ◽  
Guillemo Picó
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Isolation Method ◽  
Enzyme Isolation

Spectral and hydrodynamic studies of complex formation of tetraalkoxy substituted zinc(II)phthalocyanines with defatted and nondefatted bovine serum albumin

2015 ◽  
Vol 10 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Natalya Lebedeva ◽  
Elena Malkova ◽  
Anatoly Vyugin ◽  
Oscar Koifman ◽  
Yury Gubarev
Keyword(s):  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Bovine Serum
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