Enhancing the Toughness of Regenerated Silk Fibroin Film through Uniaxial Extension

2010 ◽  
Vol 11 (11) ◽  
pp. 2890-2895 ◽  
Author(s):  
Jianwei Yin ◽  
Erqiang Chen ◽  
David Porter ◽  
Zhengzhong Shao
Keyword(s):  
Silk Fibroin ◽  
Uniaxial Extension ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film

Genotoxic Potential of Regenerated Silk Fibroin Films by Different Cross-Linking Mode

2007 ◽  
Vol 342-343 ◽  
pp. 257-260 ◽  
Author(s):  
Jing Cheng Miao ◽  
Wei Hua Sheng ◽  
Ming Zhong Li ◽  
Yu Feng Xie ◽  
Ai Hua Gong ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Cross Linking ◽  
Control Group ◽  
Blank Group ◽  
Genotoxic Potential ◽  
Single Cell Gel Electrophoresis ◽  
Naturally Occurring ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film ◽  
Better Than

Emerging studies have showed that silk fibroin might be as a naturally occurring degradable biomaterial for tissue engineering with unique mechanical properties, biocompatib ility and processability. To optimize the formation of the regenerated silk fibroin film, in thi s study, we examined the genotoxic potential of the regenerated silk fibroin films by differe nt cross-linking mode with micronucleus and the single cell gel electrophoresis (comet) assa y. The data showed that MNF in group PC and group SD showed significantly no differenc e with blank group (P>0.05), and no evident comet tails were observed. However, the resul t of EC group was significantly different from the control group(P<0.01). Taken together, R SFFs in group PC and group SD have no evident genotoxicity, which is better than group EC.

Preparation of a regenerated silk fibroin film and its adsorbability to azo dyes

2017 ◽  
Vol 102 ◽  
pp. 1066-1072 ◽  
Author(s):  
Peng Song ◽  
Dong-Yang Zhang ◽  
Xiao-Hui Yao ◽  
Fan Feng ◽  
Guo-Hua Wu
Keyword(s):  
Silk Fibroin ◽  
Azo Dyes ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film

scholarly journals Chondrocytes cultured in silk-based biomaterials maintain function and cell morphology

2018 ◽  
Vol 42 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Yusu Ni ◽  
Yi Jiang ◽  
Kaishi Wang ◽  
Zhengzhong Shao ◽  
Xin Chen ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Cell Morphology ◽  
Laser Scanning ◽  
Fluorescent Protein ◽  
Cartilage Defects ◽  
Protein Marker ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film ◽  
Collagen Ii

Objective: To characterize the morphology of chondrocytes and the expression and secretion of active collagen II by these cells cultured within a regenerated silk fibroin film. Silk fibroin film cytocompatibility and the effect of silk fibroin on chondrocytes in vitro were also evaluated. Methods: Chondrocytes were transfected with a lentivirus containing a green fluorescent protein marker and cultured within a regenerated silk fibroin film. Effects on chondrocyte adhesion, growth, and expression of functional collagen II were assessed in vitro by analysis with immunofluorescent histochemistry and laser scanning confocal microscopy. Results: The results of this study showed that the regenerated silk fibroin film had no cytotoxic effect on chondrocytes. The regenerated silk fibroin film facilitated the adhesion of chondrocytes with typical morphology. Chondrocytes cultured within silk fibroin films exhibited the expression of collagen II in vitro. Conclusion: Regenerated silk fibroin film was found to be an excellent biomaterial with good cytocompatibility for chondrocytes, because these cells remained functional and maintained normal cell morphology when cultured in silk-based biomaterials. These results suggest that silk-based chondrocyte biomaterial complexes may provide a feasible and functional biomaterial for repairing clinical cartilage defects.

Microsegregated structure in the regenerated silk fibroin film from bombyx mori

1987 ◽  
Vol 25 (12) ◽  
pp. 2567-2571 ◽  
Author(s):  
M. Nagura ◽  
T. Ueki ◽  
Y. Hiragi ◽  
H. Tagawa ◽  
M. Kataoka ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film

scholarly journals Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

2010 ◽  
Vol 11 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Tie-lian Liu ◽  
Jing-cheng Miao ◽  
Wei-hua Sheng ◽  
Yu-feng Xie ◽  
Quan Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Film

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Sign in / Sign up

Export Citation Format

Share Document