Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration

2014 ◽  
Vol 15 (6) ◽  
pp. 2019-2026 ◽  
Author(s):  
Agnieszka K. Muszanska ◽  
Edward T. J. Rochford ◽  
Agnieszka Gruszka ◽  
Andreas A. Bastian ◽  
Henk J. Busscher ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Polymer Brush ◽  
Rgd Peptides ◽  
Tissue Integration ◽  
Brush Coating

scholarly journals Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid

Coatings ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 398 ◽  
Author(s):  
Hideyuki Kanematsu ◽  
Atsuya Oizumi ◽  
Takaya Sato ◽  
Toshio Kamijo ◽  
Saika Honma ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Polymethyl Methacrylate ◽  
Biofilm Formation ◽  
Polymer Brush ◽  
E Coli ◽  
Glass Substrates ◽  
Liquid Type ◽  
Brush Coating

N,N-diethyl-N-(2-methancryloylethy)-N-methylammonium bis(trifluoromethylsulfonyl) imide polymer (DEMM-TFSI) brush coated specimens (substrate: glasses) and a liquid ion type of polymer brush coating were investigated for their antifouling effect on biofilms. Biofilms were produced by two kinds of bacteria, E. coli and S. epidermidis. They were formed on specimens immersed into wells (of 12-well plates) that were filled with culture liquids and bacteria. The biofilm formation was observed. Also, brush coated specimens and glass substrates were investigated in the same way. DEMM polymer brush coated specimens formed more biofilm than PMMA (polymethyl methacrylate) polymer brush coated specimens and glass substrates. A greater amount of polarized components of biofilms was also observed for DEMM polymer brush coated specimens. The polar characteristics could be attributed to the attraction capability of bacteria and biofilms on DEMM polymer brush coated specimens. When considering the ease of removing biofilms by washing it with water, the ionic liquid type polymer brush (coated specimens) could be used for antifouling applications. If an initial antifouling application is needed, then the polar characteristics could be adjusted (design of the components and concentrations of ionic liquids, etc.) to solve the problem.

Microbial biofilm growth versus tissue integration on biomaterials with different wettabilities and a polymer-brush coating

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Guruprakash Subbiahdoss ◽  
Dirk W. Grijpma ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
Roel Kuijer
Keyword(s):  
Polymer Brush ◽  
Microbial Biofilm ◽  
Biofilm Growth ◽  
Tissue Integration ◽  
Brush Coating

scholarly journals Live cell micropatterning reveals the dynamics of signaling complexes at the plasma membrane

2014 ◽  
Vol 207 (3) ◽  
pp. 407-418 ◽  
Author(s):  
Sara Löchte ◽  
Sharon Waichman ◽  
Oliver Beutel ◽  
Changjiang You ◽  
Jacob Piehler
Keyword(s):  
Plasma Membrane ◽  
Single Molecule ◽  
Spatial Organization ◽  
Polymer Brush ◽  
Effector Proteins ◽  
Live Cells ◽  
Type I ◽  
Rgd Peptides ◽  
Single Molecule Level ◽  
Cell Micropatterning

Interactions of proteins in the plasma membrane are notoriously challenging to study under physiological conditions. We report in this paper a generic approach for spatial organization of plasma membrane proteins into micropatterns as a tool for visualizing and quantifying interactions with extracellular, intracellular, and transmembrane proteins in live cells. Based on a protein-repellent poly(ethylene glycol) polymer brush, micropatterned surface functionalization with the HaloTag ligand for capturing HaloTag fusion proteins and RGD peptides promoting cell adhesion was devised. Efficient micropatterning of the type I interferon (IFN) receptor subunit IFNAR2 fused to the HaloTag was achieved, and highly specific IFN binding to the receptor was detected. The dynamics of this interaction could be quantified on the single molecule level, and IFN-induced receptor dimerization in micropatterns could be monitored. Assembly of active signaling complexes was confirmed by immunostaining of phosphorylated Janus family kinases, and the interaction dynamics of cytosolic effector proteins recruited to the receptor complex were unambiguously quantified by fluorescence recovery after photobleaching.

Bacterial adhesion and growth on a polymer brush-coating

Biomaterials ◽  
2008 ◽  
Vol 29 (30) ◽  
pp. 4117-4121 ◽  
Author(s):  
M. Reza Nejadnik ◽  
Henny C. van der Mei ◽  
Willem Norde ◽  
Henk J. Busscher
Keyword(s):  
Bacterial Adhesion ◽  
Polymer Brush ◽  
Brush Coating

A hierarchical polymer brush coating with dual-function antibacterial capability

2017 ◽  
Vol 149 ◽  
pp. 260-270 ◽  
Author(s):  
Shunjie Yan ◽  
Lingjie Song ◽  
Shifang Luan ◽  
Zhirong Xin ◽  
Shanshan Du ◽  
...  
Keyword(s):  
Polymer Brush ◽  
Dual Function ◽  
Brush Coating

Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor

2020 ◽  
Vol MA2020-01 (43) ◽  
pp. 2519-2519
Author(s):  
Hideyuki Kanematsu ◽  
Atsuya Oizumi ◽  
Takaya Sato ◽  
Ryo Sato ◽  
Toshio Kamijo ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Biofilm Formation ◽  
Polymer Brush ◽  
Biofilm Reactor ◽  
Flow Type

scholarly journals Determination of the Shear Force at the Balance between Bacterial Attachment and Detachment in Weak-Adherence Systems, Using a Flow Displacement Chamber

2007 ◽  
Vol 74 (3) ◽  
pp. 916-919 ◽  
Author(s):  
M. Reza Nejadnik ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
Willem Norde
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Shear Force ◽  
Polymer Brush ◽  
Bacterial Attachment ◽  
Adhesion Forces ◽  
Shear Forces ◽  
Flow Displacement

ABSTRACT We introduce a procedure for determining shear forces at the balance between attachment and detachment of bacteria under flow. This procedure can be applied to derive adhesion forces in weak-adherence systems, such as polymer brush coatings, which are currently at the center of attention for their control of bacterial adhesion and biofilm formation.

Polymer Brush Made by Ionic Liquids and the Inhibition Effects for Biofilm Formation

2018 ◽  
Vol 85 (13) ◽  
pp. 1089-1095 ◽  
Author(s):  
Hideyuki Kanematsu ◽  
Atsuya Oizumi ◽  
Takaya Sato ◽  
Toshio Kamijo ◽  
Saika Honma ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Biofilm Formation ◽  
Polymer Brush

Methacrylate-ended polypeptides and polypeptoids for antimicrobial and antifouling coatings

2017 ◽  
Vol 8 (41) ◽  
pp. 6386-6397 ◽  
Author(s):  
Qiang Gao ◽  
Peng Li ◽  
Hongyang Zhao ◽  
Yashao Chen ◽  
Liu Jiang ◽  
...  
Keyword(s):  
Polymer Brush ◽  
Antifouling Coatings ◽  
Brush Coating

Methacrylate-terminated polypept(o)ides were directly synthesized via NCA-ROP, and then surface-grafted to form a polymer brush coating with infection-resistant efficacy.

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