Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid

N,N-diethyl-N-(2-methancryloylethy)-N-methylammonium bis(trifluoromethylsulfonyl) imide polymer (DEMM-TFSI) brush coated specimens (substrate: glasses) and a liquid ion type of polymer brush coating were investigated for their antifouling effect on biofilms. Biofilms were produced by two kinds of bacteria, E. coli and S. epidermidis. They were formed on specimens immersed into wells (of 12-well plates) that were filled with culture liquids and bacteria. The biofilm formation was observed. Also, brush coated specimens and glass substrates were investigated in the same way. DEMM polymer brush coated specimens formed more biofilm than PMMA (polymethyl methacrylate) polymer brush coated specimens and glass substrates. A greater amount of polarized components of biofilms was also observed for DEMM polymer brush coated specimens. The polar characteristics could be attributed to the attraction capability of bacteria and biofilms on DEMM polymer brush coated specimens. When considering the ease of removing biofilms by washing it with water, the ionic liquid type polymer brush (coated specimens) could be used for antifouling applications. If an initial antifouling application is needed, then the polar characteristics could be adjusted (design of the components and concentrations of ionic liquids, etc.) to solve the problem.

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Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽

10.1039/c6ra16722e ◽

2016 ◽

Vol 6 (86) ◽

pp. 82571-82580 ◽

Cited By ~ 4

Author(s):

Muhammad Hakimi Hadzir ◽

Sahar Abbasiliasi ◽

Arbakariya B. Ariff ◽

Siti Baidurah Yusoff ◽

Hui Suan Ng ◽

...

Keyword(s):

Escherichia Coli ◽

Ionic Liquid ◽

Ionic Liquids ◽

Fermentation Broth ◽

Two Phase ◽

E Coli ◽

Recombinant Lipase ◽

Aqueous Two Phase Systems ◽

Phase Systems ◽

Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

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Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor

ECS Meeting Abstracts ◽

10.1149/ma2020-01432519mtgabs ◽

2020 ◽

Vol MA2020-01 (43) ◽

pp. 2519-2519

Author(s):

Hideyuki Kanematsu ◽

Atsuya Oizumi ◽

Takaya Sato ◽

Ryo Sato ◽

Toshio Kamijo ◽

...

Keyword(s):

Ionic Liquid ◽

Biofilm Formation ◽

Polymer Brush ◽

Biofilm Reactor ◽

Flow Type

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Polymer Brush Made by Ionic Liquids and the Inhibition Effects for Biofilm Formation

ECS Transactions ◽

10.1149/08513.1089ecst ◽

2018 ◽

Vol 85 (13) ◽

pp. 1089-1095 ◽

Cited By ~ 2

Author(s):

Hideyuki Kanematsu ◽

Atsuya Oizumi ◽

Takaya Sato ◽

Toshio Kamijo ◽

Saika Honma ◽

...

Keyword(s):

Ionic Liquids ◽

Biofilm Formation ◽

Polymer Brush

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Antibiofilm activity of tert-BuOH functionalized ionic liquids with methylsulfonate counteranions

RSC Advances ◽

10.1039/c5ra12854d ◽

2015 ◽

Vol 5 (83) ◽

pp. 68136-68142 ◽

Cited By ~ 11

Author(s):

Govinda R. Navale ◽

Mahesh S. Dharne ◽

Sandip S. Shinde

Keyword(s):

Antimicrobial Activity ◽

Ionic Liquid ◽

Ionic Liquids ◽

Biofilm Formation ◽

Alkyl Chain ◽

Antibiofilm Activity ◽

Imidazolium Ionic Liquid ◽

Potential Antimicrobial Activity

Thetert-BuOH functionalized and dodecyl alkyl chain bearing imidazolium ionic liquid [C12-tOHim][OMs] significantly prevented biofilm formation ofS. epidermidisand showed potential antimicrobial activity.

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Prospects for the Application of Scanning Electrochemical Microscopy in Ionic Liquids

Australian Journal of Chemistry ◽

10.1071/ch06269 ◽

2007 ◽

Vol 60 (1) ◽

pp. 29 ◽

Cited By ~ 17

Author(s):

Maurizio Carano ◽

Alan M. Bond

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Steady State ◽

Aqueous Media ◽

Scanning Electrochemical Microscopy ◽

State Theory ◽

Glass Substrates ◽

State Behaviour ◽

Ag Substrate ◽

Electrochemical Microscopy

Prospects have been assessed for the application of the technique of scanning electrochemical microscopy (SECM) in viscous ionic liquids. Use of the Fc0/+ redox couple (Fc = ferrocene) to obtain microelectrode approach curves revealed that the required steady state behaviour needed for analysis of data relevant to conducting platinum and insulating glass substrates is moderately easy to achieve in a 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]) ionic liquid. However, in the more highly viscous 1-butyl-3-methylimidazolium hexafluorophosphate ionic liquid, where significantly lower diffusion coefficients are encountered, approach-curve data that conform to steady state theory are only found with very small microelectrodes (e.g., ≤2 μm diameter), very slow electrode–substrate approach rates (e.g., ≤0.05 μm s–1), and long equilibration times (e.g., 50 s). A comparison is provided with SECM approach curves acquired in aqueous media. SECM images of an Ag substrate in contact with [BMIM][BF4] also are presented and are of only slightly inferior quality to images obtained in water.

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Efficacy of A Poly(MeOEGMA) Brush on the Prevention of Escherichia coli Biofilm Formation and Susceptibility

Antibiotics ◽

10.3390/antibiotics9050216 ◽

2020 ◽

Vol 9 (5) ◽

pp. 216 ◽

Cited By ~ 1

Author(s):

Patrícia Alves ◽

Luciana Calheiros Gomes ◽

Cesar Rodríguez-Emmenegger ◽

Filipe José Mergulhão

Keyword(s):

Escherichia Coli ◽

Urinary Tract ◽

Biofilm Formation ◽

Polymer Brush ◽

Cell Number ◽

Flow Chamber ◽

E Coli ◽

Tract Infections ◽

Hospital Acquired ◽

Control Surfaces

Urinary tract infections are one of the most common hospital-acquired infections, and they are often associated with biofilm formation in indwelling medical devices such as catheters and stents. This study aims to investigate the antibiofilm performance of a polymer brush—poly[oligo(ethylene glycol) methyl ether methacrylate], poly(MeOEGMA)—and evaluate its effect on the antimicrobial susceptibility of Escherichia coli biofilms formed on that surface. Biofilms were formed in a parallel plate flow chamber (PPFC) for 24 h under the hydrodynamic conditions prevailing in urinary catheters and stents and challenged with ampicillin. Results obtained with the brush were compared to those obtained with two control surfaces, polydimethylsiloxane (PDMS) and glass. The polymer brush reduced by 57% the surface area covered by E. coli after 24 h, as well as the number of total adhered cells. The antibiotic treatment potentiated cell death and removal, and the total cell number was reduced by 88%. Biofilms adapted their architecture, and cell morphology changed to a more elongated form during that period. This work suggests that the poly(MeOEGMA) brush has potential to prevent bacterial adhesion in urinary tract devices like ureteral stents and catheters, as well as in eradicating biofilms developed in these biomedical devices.

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Evaluation of mammalian and bacterial cell activity on titanium surface coated with dicationic imidazolium-based ionic liquids

RSC Advances ◽

10.1039/c6ra01003b ◽

2016 ◽

Vol 6 (43) ◽

pp. 36475-36483 ◽

Cited By ~ 11

Author(s):

Izabelle M. Gindri ◽

Kelli L. Palmer ◽

Danyal A. Siddiqui ◽

Shant Aghyarian ◽

Clarissa P. Frizzo ◽

...

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Bacterial Cell ◽

Biofilm Formation ◽

Titanium Surface ◽

Bacterial Colonization ◽

Cell Activity ◽

New Strategy ◽

Titanium Surfaces

This work presents a new strategy to protect titanium surfaces against bacterial colonization and biofilm formation using dicationic imidazolium-based ionic liquid coatings.

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