Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations

1993 ◽  
Vol 9 (6) ◽  
pp. 671-674 ◽  
Author(s):  
Steven A. Altman ◽  
Lisa Randers ◽  
Govind Rao
2010 ◽  
Vol 9 (3) ◽  
pp. 354-359 ◽  
Author(s):  
Sandra Kalil Bussadori ◽  
Kristianne Porta Santos Fernandes ◽  
Raquel Agnelli Mesquita-Ferrari ◽  
Elaine Marcilio Santos ◽  
Carolina Cardoso Guedes ◽  
...  

Introduction: Casein phosphopeptides (CPP) have been shown to be good carriers of calcium, phosphate, and hydroxide ions to promote enamel remineralization with applications in oral care products, professional dental products, and food products. Objectives: Evaluate the cytotoxicity of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste in rat fibroblasts. Materials and methods: Cytotoxicity was measured by the Trypan blue dye exclusion assay and the MTT assay. Results: Long term (1, 3, 5, and 7 days) and short term tests (0, 4, 8, and 12 hours) were performed with the Trypan blue dye exclusion assay. Data were analyzed with ANOVA and Kruskall Wallis tests. After one day, the cell viability of the CPP-ACP study group was between 80 and 100%. In the short term experiments, a reduction of cell viability in the study group was observed compared with the control group; yet viable cells were present over the evaluation period. With the MTT assay, a viability of 76.43% was observed in the study group, a significant difference from the control group (P = 0.003). Conclusion: CPP-ACP paste demonstrates low cytotoxicity in rat fibroblast culture.


2021 ◽  
Author(s):  
Anand Kumar Awasthi ◽  
Sakshi Gupta ◽  
Kavthekar Rupesh Namdev ◽  
Aditi Banerjee ◽  
Aasheesh Srivastava

Polydopamine (PDA) and dopamine (DA) can spontaneously reduce MTT reagent to formazan, resulting in incorrect cell-viability inferences. The non-redox Trypan Blue exclusion assay provides a more reliable estimate of cell viability with PDA and DA.


1993 ◽  
Vol 21 (3) ◽  
pp. 324-329
Author(s):  
Jeffrey R. Fry ◽  
Alison H. Hammond

A variety of approaches to assessment of cellular integrity exist, based on tests of integrity of the plasma membrane, tests of metabolic competence, and asessment of morphology. By definition, such approaches address different aspects of cellular integrity and hence are not interchangeable indices of cellular integrity. Accordingly, it would be most appropriate to characterise hepatocyte preparations on the basis of more than just trypan blue dye exclusion (a test of plasma membrane integrity) as is customary. A scheme for the choice of the most appropriate mix of tests of cellular integrity is presented.


Author(s):  
Xavier Ronot ◽  
Sylvain Paillasson ◽  
Katharine A. Muirhead

2003 ◽  
Vol 29 (9) ◽  
pp. 1733-1737 ◽  
Author(s):  
Jagjit S. Saini ◽  
Arun K. Jain ◽  
Jaspreet Sukhija ◽  
Poonam Gupta ◽  
Vandana Saroha

Author(s):  
Xavier Ronot ◽  
Sylvain Paillasson ◽  
Katharine A. Muirhead

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