Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations

Introduction: Casein phosphopeptides (CPP) have been shown to be good carriers of calcium, phosphate, and hydroxide ions to promote enamel remineralization with applications in oral care products, professional dental products, and food products. Objectives: Evaluate the cytotoxicity of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste in rat fibroblasts. Materials and methods: Cytotoxicity was measured by the Trypan blue dye exclusion assay and the MTT assay. Results: Long term (1, 3, 5, and 7 days) and short term tests (0, 4, 8, and 12 hours) were performed with the Trypan blue dye exclusion assay. Data were analyzed with ANOVA and Kruskall Wallis tests. After one day, the cell viability of the CPP-ACP study group was between 80 and 100%. In the short term experiments, a reduction of cell viability in the study group was observed compared with the control group; yet viable cells were present over the evaluation period. With the MTT assay, a viability of 76.43% was observed in the study group, a significant difference from the control group (P = 0.003). Conclusion: CPP-ACP paste demonstrates low cytotoxicity in rat fibroblast culture.

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Polydopamine and dopamine interfere with tetrazolium-based cytotoxicity assays and produce exaggerated cytocompatibility inferences

Biomaterials Science ◽

10.1039/d1bm00140j ◽

2021 ◽

Author(s):

Anand Kumar Awasthi ◽

Sakshi Gupta ◽

Kavthekar Rupesh Namdev ◽

Aditi Banerjee ◽

Aasheesh Srivastava

Keyword(s):

Cell Viability ◽

Trypan Blue ◽

Reliable Estimate ◽

Trypan Blue Exclusion ◽

Cytotoxicity Assays ◽

Trypan Blue Exclusion Assay

Polydopamine (PDA) and dopamine (DA) can spontaneously reduce MTT reagent to formazan, resulting in incorrect cell-viability inferences. The non-redox Trypan Blue exclusion assay provides a more reliable estimate of cell viability with PDA and DA.

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Unintentional staining of the posterior lens capsule with trypan blue dye during phacoemulsification: case report

International Ophthalmology ◽

10.1007/s10792-012-9534-4 ◽

2012 ◽

Vol 32 (2) ◽

pp. 187-189 ◽

Cited By ~ 3

Author(s):

Aysel Pelit

Keyword(s):

Case Report ◽

Trypan Blue ◽

Lens Capsule ◽

Blue Dye

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Assessment of the Functional Integrity of Hepatocytes: A Brief Review

Alternatives to Laboratory Animals ◽

10.1177/026119299302100303 ◽

1993 ◽

Vol 21 (3) ◽

pp. 324-329

Author(s):

Jeffrey R. Fry ◽

Alison H. Hammond

Keyword(s):

Plasma Membrane ◽

Trypan Blue ◽

Membrane Integrity ◽

Blue Dye ◽

Plasma Membrane Integrity ◽

Functional Integrity ◽

Cellular Integrity

A variety of approaches to assessment of cellular integrity exist, based on tests of integrity of the plasma membrane, tests of metabolic competence, and asessment of morphology. By definition, such approaches address different aspects of cellular integrity and hence are not interchangeable indices of cellular integrity. Accordingly, it would be most appropriate to characterise hepatocyte preparations on the basis of more than just trypan blue dye exclusion (a test of plasma membrane integrity) as is customary. A scheme for the choice of the most appropriate mix of tests of cellular integrity is presented.

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