Constitutive overexpression of secreted heterologous proteins decreases extractable heavy chain binding protein and protein disulfide isomerase levels in Saccharomyces cerevisiae

1995 ◽  
Vol 11 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Anne Skaja Robinson ◽  
K. Dane Wittrup
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Chain ◽  
Protein Disulfide Isomerase ◽  
Binding Protein ◽  
Heterologous Proteins ◽  
Disulfide Isomerase ◽  
Constitutive Overexpression ◽  
Protein Disulfide

The yeast EUG1 gene encodes an endoplasmic reticulum protein that is functionally related to protein disulfide isomerase

1992 ◽  
Vol 12 (10) ◽  
pp. 4601-4611
Author(s):  
C Tachibana ◽  
T H Stevens
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Endoplasmic Reticulum ◽  
Protein Disulfide Isomerase ◽  
Yeast Cells ◽  
Disulfide Isomerase ◽  
Protein Levels ◽  
Growth Defects ◽  
Endoplasmic Reticulum Protein ◽  
Related Proteins ◽  
Protein Disulfide

The product of the EUG1 gene of Saccharomyces cerevisiae is a soluble endoplasmic reticulum protein with homology to both the mammalian protein disulfide isomerase (PDI) and the yeast PDI homolog encoded by the essential PDI1 gene. Deletion or overexpression of EUG1 causes no growth defects under a variety of conditions. EUG1 mRNA and protein levels are dramatically increased in response to the accumulation of native or unglycosylated proteins in the endoplasmic reticulum. Overexpression of the EUG1 gene allows yeast cells to grow in the absence of the PDI1 gene product. Depletion of the PDI1 protein in Saccharomyces cerevisiae causes a soluble vacuolar glycoprotein to accumulate in its endoplasmic reticulum form, and this phenotype is only partially relieved by the overexpression of EUG1. Taken together, our results indicate that PDI1 and EUG1 encode functionally related proteins that are likely to be involved in interacting with nascent polypeptides in the yeast endoplasmic reticulum.

Production of Rat Protein Disulfide Isomerase in Saccharomyces cerevisiae

1995 ◽  
Vol 6 (5) ◽  
pp. 700-706 ◽  
Author(s):  
M.C.A. Laboissiere ◽  
P.T. Chivers ◽  
R.T. Raines
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Protein Disulfide

scholarly journals The b’x Region of Yeast Protein Disulfide Isomerase is Not Essential for Saccharomyces cerevisiae Viability at 30 °C

2009 ◽  
Vol 3 (1) ◽  
pp. 27-32
Author(s):  
PURKAN PURKAN ◽  
LALU RUDYAT TELLY SAVALAS ◽  
MULIAWATI SINDUMARTA ◽  
DESSY NATALIA
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Disulfide Isomerase ◽  
Yeast Protein ◽  
Disulfide Isomerase ◽  
Protein Disulfide

Protein disulfide isomerase is essential for viability in Saccharomyces cerevisiae

Gene ◽  
1991 ◽  
Vol 108 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Ronnie Farquhar ◽  
Neville Honey ◽  
Susan J. Murant ◽  
Peter Bossier ◽  
Loren Schultz ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Disulfide Isomerase ◽  
Disulfide Isomerase ◽  
Protein Disulfide
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