Nonviral Vectors for Gene Delivery

2009 ◽  
Vol 109 (2) ◽  
pp. 259-302 ◽  
Author(s):  
Meredith A. Mintzer ◽  
Eric E. Simanek
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors

Chitosan-Based Nonviral Vectors for Gene Delivery

2005 ◽  
pp. 817-836
Author(s):  
Wen Guang Liu ◽  
William Lu ◽  
Kang De Yao
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors

scholarly journals Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ana V. Oliveira ◽  
Diogo B. Bitoque ◽  
Gabriela A. Silva
Keyword(s):  
Hyaluronic Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Nonviral Vectors ◽  
Anionic Polymer ◽  
Dna Release

The low gene transfer efficiency of chitosan-DNA polyplexes is a consequence of their high stability and consequent slow DNA release. The incorporation of an anionic polymer is believed to loosen chitosan interactions with DNA and thus promote higher transfection efficiencies. In this work, several formulations of chitosan-DNA polyplexes incorporating hyaluronic acid were prepared and characterized for their gene transfection efficiency on both HEK293 and retinal pigment epithelial cells. The different polyplex formulations showed morphology, size, and charge compatible with a role in gene delivery. The incorporation of hyaluronic acid rendered the formulations less stable, as was the goal, but it did not affect the loading and protection of the DNA. Compared with chitosan alone, the transfection efficiency had a 4-fold improvement, which was attributed to the presence of hyaluronic acid. Overall, our hybrid chitosan-hyaluronic acid polyplexes showed a significant improvement of the efficiency of chitosan-based nonviral vectorsin vitro, suggesting that this strategy can further improve the transfection efficiency of nonviral vectors.

scholarly journals Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors

2016 ◽  
Vol 34 (2) ◽  
pp. 91-105 ◽  
Author(s):  
Andrew B. Hill ◽  
Mingfu Chen ◽  
Chih-Kuang Chen ◽  
Blaine A. Pfeifer ◽  
Charles H. Jones
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors

Physicochemical properties of low molecular weight alkylated chitosans: A new class of potential nonviral vectors for gene delivery

2006 ◽  
Vol 51 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Sebnem Ercelen ◽  
Xin Zhang ◽  
Guy Duportail ◽  
Christian Grandfils ◽  
Jacques Desbrières ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Physicochemical Properties ◽  
Low Molecular Weight ◽  
Nonviral Vectors ◽  
New Class

Gene Transfer Using Nonviral Delivery Systems

2006 ◽  
Vol 26 (6) ◽  
pp. 633-640 ◽  
Author(s):  
Masanobu Miyazaki ◽  
Yoko Obata ◽  
Katsushige Abe ◽  
Akira Furusu ◽  
Takehiko Koji ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Peritoneal Dialysis ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Nonviral Vectors ◽  
Gene Delivery System ◽  
Peritoneal Membrane ◽  
Peritoneal Function

In peritoneal dialysis, loss of peritoneal function is a major factor in treatment failure. The alterations in peritoneal function are related to structural changes in the peritoneal membrane, including peritoneal sclerosis with increased extracellular matrix. Although peritoneal sclerosis is considered reversible to some extent through peritoneal rest, which improves peritoneal function and facilitates morphological changes, there has been no therapeutic intervention and no drug against the development and progression of peritoneal sclerosis. Using recent biotechnological advances in genetic engineering, a strategy based on genetic modification of the peritoneal membrane could be a potential therapeutic maneuver against peritoneal sclerosis and peritoneal membrane failure. Before this gene therapy may be applied clinically, a safe and effective gene delivery system as well as the selection of a gene therapy method must be established. There are presently two kinds of gene transfer vectors: viral and nonviral. Viral vectors are used mainly as a gene delivery system in the field of continuous ambulatory peritoneal dialysis research; however, they have several problems such as immunogenicity and toxicity. On the other hand, nonviral vectors have several advantages over viral vectors. We review here gene transfer using nonviral vector systems in the peritoneum: electroporation, liposomes, and cationized gelatin microspheres. In the field of peritoneal dialysis, gene therapy research using nonviral vectors is presently limited. Improvement in delivery methods together with an intelligent design of targeted genes has brought about large degrees of enhancement in the efficiency, specificity, and temporal control of nonviral vectors.

scholarly journals Enzyme-synthesized poly(amine-co-esters) as nonviral vectors for gene delivery

2010 ◽  
Vol 96A (2) ◽  
pp. 456-465 ◽  
Author(s):  
Jie Liu ◽  
Zhaozhong Jiang ◽  
Jiangbing Zhou ◽  
Shengmin Zhang ◽  
W. Mark Saltzman
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors

Dual-Functionalized Covalent Triazine Framework Nanosheets as Hierarchical Nonviral Vectors for Intracellular Gene Delivery

2021 ◽  
Author(s):  
Yaqi Cao ◽  
Junshi Zhang ◽  
Lan Wang ◽  
Mingjun Cen ◽  
Wenchao Peng ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nonviral Vectors ◽  
Covalent Triazine Framework
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