scholarly journals Data-Independent Acquisition Mass Spectrometry of the Human Lens Enhances Spatiotemporal Measurement of Fiber Cell Aging

2021 ◽  
Author(s):  
Lee S. Cantrell ◽  
Kevin L. Schey
Keyword(s):  
Mass Spectrometry ◽  
Fiber Cell ◽  
Human Lens ◽  
Cell Aging ◽  
Data Independent Acquisition

scholarly journals Data Independent Acquisition Mass Spectrometry of the Human Lens Enhances Spatiotemporal Measurement of Fiber Cell Aging

2021 ◽  
Author(s):  
Lee S Cantrell ◽  
Kevin L Schey
Keyword(s):  
Dynamic Range ◽  
High Dynamic Range ◽  
Fiber Cell ◽  
Human Lens ◽  
Cell Aging ◽  
Fiber Cells ◽  
Data Independent Acquisition ◽  
Age Related ◽  
Cell Age ◽  
Independent Analysis

The ocular lens proteome undergoes post-translational and progressive degradation as fiber cells age. The oldest fiber cells and the proteins therein are present at birth and are retained through death. Transparency of the lens is maintained in part by the high abundance crystallin family proteins (up to 300 mg/mL), which establishes a high dynamic range of protein abundance. As a result, previous Data Dependent Analysis (DDA) measurements of the lens proteome are less equipped to identify the lowest abundance proteins. In an attempt to probe more deeply into the lens proteome, we measured the insoluble lens proteome of an 18-year-old human with DDA and newer Data Independent Analysis (DIA) methods. By applying library free DIA search methods, 4,564 protein groups, 48,474 peptides and 5,577 deamidation sites were detected: significantly outperforming the quantity of identifications in using DDA and Pan-Human DIA library searches. Finally, by segmenting the lens into multiple fiber cell-age related regions, we uncovered cell-age resolved changes in proteome composition and putative function.

scholarly journals Direct injection high performance liquid chromatography coupled to data independent acquisition mass spectrometry for the screening of antibiotics in honey

2019 ◽  
Vol 27 (3) ◽  
pp. 679-691 ◽  
Author(s):  
Annie von Eyken ◽  
Daniel Furlong ◽  
Samareh Arooni ◽  
Fred Butterworth ◽  
Jean-François Roy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Direct Injection ◽  
Data Independent Acquisition

High‐throughput, in‐depth and estimated absolute quantification of plasma proteome using data‐independent acquisition/mass spectrometry (“HIAP‐DIA”)

PROTEOMICS ◽  
2021 ◽  
pp. 2000264
Author(s):  
Yue Zhou ◽  
Zengqi Tan ◽  
Peng Xue ◽  
Yi Wang ◽  
Xiang Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Absolute Quantification ◽  
Plasma Proteome ◽  
Data Independent Acquisition ◽  
Using Data

Assigning in vivo carbamylation and acetylation in human lens proteins using tandem mass spectrometry and database searching

2007 ◽  
Vol 259 (1-3) ◽  
pp. 161-173 ◽  
Author(s):  
Zee-Yong Park ◽  
Rovshan Sadygov ◽  
Judy M. Clark ◽  
John I. Clark ◽  
John R. Yates
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Human Lens ◽  
Database Searching ◽  
Tandem Mass ◽  
Lens Proteins

scholarly journals Proteomic insights into synaptic signaling in the brain: the past, present and future

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yalan Xu ◽  
Xiuyue Song ◽  
Dong Wang ◽  
Yin Wang ◽  
Peifeng Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Complexes ◽  
Synaptic Signaling ◽  
Brain Functions ◽  
The Past ◽  
Data Independent Acquisition ◽  
Technological Advances ◽  
Neural Communication ◽  
Chemical Synapses ◽  
The Brain

AbstractChemical synapses in the brain connect neurons to form neural circuits, providing the structural and functional bases for neural communication. Disrupted synaptic signaling is closely related to a variety of neurological and psychiatric disorders. In the past two decades, proteomics has blossomed as a versatile tool in biological and biomedical research, rendering a wealth of information toward decoding the molecular machinery of life. There is enormous interest in employing proteomic approaches for the study of synapses, and substantial progress has been made. Here, we review the findings of proteomic studies of chemical synapses in the brain, with special attention paid to the key players in synaptic signaling, i.e., the synaptic protein complexes and their post-translational modifications. Looking toward the future, we discuss the technological advances in proteomics such as data-independent acquisition mass spectrometry (DIA-MS), cross-linking in combination with mass spectrometry (CXMS), and proximity proteomics, along with their potential to untangle the mystery of how the brain functions at the molecular level. Last but not least, we introduce the newly developed synaptomic methods. These methods and their successful applications marked the beginnings of the synaptomics era.

Age-Related Changes in Human Lens Crystallins Identified by HPLC and Mass Spectrometry

1998 ◽  
Vol 67 (1) ◽  
pp. 21-30 ◽  
Author(s):  
ZHIXIANG MA ◽  
STACY R.A. HANSON ◽  
KIRSTEN J. LAMPI ◽  
LARRY L. DAVID ◽  
DAVID L. SMITH ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Lens ◽  
Lens Crystallins ◽  
Age Related ◽  
Age Related Changes

Structure Elucidation of Human Lens Proteins by Mass Spectrometry

1996 ◽  
pp. 223-235
Author(s):  
David L. Smith ◽  
Peiping Lin ◽  
Anders Lund ◽  
Jean B. Smith
Keyword(s):  
Mass Spectrometry ◽  
Structure Elucidation ◽  
Human Lens ◽  
Lens Proteins

scholarly journals Data-Independent Acquisition Mass Spectrometry To Quantify Protein Levels in FFPE Tumor Biopsies for Molecular Diagnostics

2018 ◽  
Author(s):  
Yeoun Jin Kim ◽  
Steve M. M. Sweet ◽  
Jarrett D. Egertson ◽  
Andrew J. Sedgewick ◽  
Sunghee Woo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Diagnostics ◽  
Protein Levels ◽  
Data Independent Acquisition ◽  
Ffpe Tumor ◽  
Tumor Biopsies
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