scholarly journals Isolation and Structural Characterization of the Milled Wood Lignin, Dioxane Lignin, and Cellulolytic Lignin Preparations from Brewer’s Spent Grain

2015 ◽  
Vol 63 (2) ◽  
pp. 603-613 ◽  
Author(s):  
Jorge Rencoret ◽  
Pepijn Prinsen ◽  
Ana Gutiérrez ◽  
Ángel T. Martı́nez ◽  
José C. del Rı́o
Keyword(s):  
Structural Characterization ◽  
Dioxane Lignin ◽  
Milled Wood Lignin ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Preparation and characterization of brewer’s spent grain protein-chitosan composite films

2015 ◽  
Vol 52 (11) ◽  
pp. 7549-7555 ◽  
Author(s):  
Ji-Hyeon Lee ◽  
Ji-Hyun Lee ◽  
Hyun-Ju Yang ◽  
Kyung Bin Song
Keyword(s):  
Composite Films ◽  
Grain Protein ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Chitosan Composite

scholarly journals Production, Purification, and Characterization of a MajorPenicillium glabrumXylanase Using Brewer's Spent Grain as Substrate

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Adriana Knob ◽  
Susan Michelz Beitel ◽  
Diana Fortkamp ◽  
César Rafael Fanchini Terrasan ◽  
Alex Fernando de Almeida
Keyword(s):  
Xylanase Activity ◽  
Stationary Condition ◽  
Purification And Characterization ◽  
Xylanase Production ◽  
Brewer’S Spent Grain ◽  
Agroindustrial Waste ◽  
Sds Page ◽  
Exclusion Chromatography ◽  
Spent Grain

In recent decades, xylanases have been used in many processing industries. This study describes the xylanase production byPenicillium glabrumusing brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained whenP. glabrumwas grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase fromP. glabrumwas purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+and the reducing agentsβ-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost.

scholarly journals EFFECT OF REACTION TIME AND SODIUM HYDROXIDE CONCENTRATION ON DELIGNIFICATION AND ENZYMATIC HYDROLYSISOF BREWER’S SPENT GRAIN FROM WO BRAZILIAN BREWERS

2021 ◽  
Vol 55 (1-2) ◽  
pp. 101-112
Author(s):  
CARLA LOBO GOMES ◽  
ELIZABETH GONÇALVES ◽  
CARLOS ALBERTO GALEANO SUAREZ ◽  
DASCIANA DE SOUSA RODRIGUES ◽  
INTI CAVALCANTI MONTANO
Keyword(s):  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Factorial Design ◽  
Contact Time ◽  
Point Contact ◽  
Sodium Hydroxide Concentration ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Hydrolysis Of

Brazil, being one of the main beer producers, generates brewer’s spent grain as a main by-product of this industry, which is mainly composed of cellulose, hemicelluloses, lignin and extractives. The alkaline pretreatment of brewer’s spent grain received from two breweries is studied in this work, namely breweries Imperial (B1) and Colombina (B2). Factorial design (22) was realized with three experiments at the central point: contact time (30, 60, 90 min) and NaOH concentration (4%, 6%, 8%). It was found that the presence of extractives causes interference in the characterization of the material. The delignification process allowed obtaining materials with lower lignin percentages when higher NaOH concentrations were used, reaching percentages of lignin loss with values between 85-95%, in both materials, but for these conditions, the losses of cellulose were considerable – of 35-43%. Enzymatic hydrolysis of the pretreated materials achieved conversions greater than 70%, emphasizing that the greatest conversions were obtained with material B1, where almost the whole cellulose was hydrolyzed.

Characterization of Lipids and Lignans in Brewer’s Spent Grain and Its Enzymatically Extracted Fraction

2012 ◽  
Vol 60 (39) ◽  
pp. 9910-9917 ◽  
Author(s):  
Piritta Niemi ◽  
Tarja Tamminen ◽  
Annika Smeds ◽  
Kaarina Viljanen ◽  
Taina Ohra-aho ◽  
...  
Keyword(s):  
Brewer’S Spent Grain ◽  
Spent Grain

scholarly journals Isolation and structural characterization of the milled-wood lignin from Paulownia fortunei wood

2009 ◽  
Vol 30 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Jorge Rencoret ◽  
Gisela Marques ◽  
Ana Gutiérrez ◽  
Lidia Nieto ◽  
J. Jiménez-Barbero ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Milled Wood Lignin ◽  
Paulownia Fortunei

Purification and structural characterization of calcium hydroxide isolated arabinoxylans derived from bran, spent grain and sorghum grains

2021 ◽  
pp. 103266
Author(s):  
Ange-Patrice Takoudjou Miafo ◽  
Gudipati Muralikrishna ◽  
Benoît Bargui Koubala ◽  
Germain Kansci
Keyword(s):  
Calcium Hydroxide ◽  
Structural Characterization ◽  
Sorghum Grains ◽  
Spent Grain

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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