Response to Comment on Extraction and Purification of Isoflavones from Soybeans and Characterization of Their Estrogenic Activities

2008 ◽  
Vol 56 (9) ◽  
pp. 3428-3430
Author(s):  
Elizabeth Jingnan Zhang ◽  
Ka Ming Ng ◽  
Kathy Qian Luo
Keyword(s):  
Extraction And Purification

scholarly journals The Enrichment And Characterization Of Compost And Wastewater-Derived Microbial Cellulolytic Consortia For Biofuel Production

2021 ◽  
Author(s):  
Augustyna Dobosz
Keyword(s):  
Energy Demand ◽  
Defined Medium ◽  
Biofuel Production ◽  
Microbial Consortia ◽  
Extraction And Purification ◽  
End Products ◽  
Fermentative Activity ◽  
Incubation Temperatures ◽  
Current Energy

Over the last decade, a rise in energy demand and diminishing fuel resources have created a challenge for finding an alternative solution that could supplement our current energy sources. This study demonstrated that ethanol and other useful end-products can be produced from the fermentative activity of microbial consortia derived from cellulose-rich waste environments. Compost and wastewater were used as inoculum sources to enrich cellulolytic cultures at incubation temperatures 50 ºC and 60ºC. A chemically defined medium was used without complex nutrients such as yeast extract. Four cellulolytic cultures were obtained and their end-products were monitored over an active cellulose degrading period. The compost culture incubated at 50ºC produced the highest concentration of butyrate while the wastewater-derived culture incubated at 60ºC produced the highest ethanol concentration. Optimization of DNA extraction and purification from complex environmental samples such as the compost and wastewater cultures used in this study was also discussed.

scholarly journals PREPARATION FROM HUMAN RED CELLS OF A SUBSTANCE INHIBITING VIRUS HEMAGGLUTINATION

1948 ◽  
Vol 87 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Patrick M. deBurgh ◽  
Pen-Chung Yu ◽  
Calderon Howe ◽  
Max Bovarnick
Keyword(s):  
Active Material ◽  
Chemical Characterization ◽  
Red Cells ◽  
Soluble Form ◽  
Red Cell ◽  
Extraction And Purification ◽  
Insoluble Material ◽  
Macromolecular Substance ◽  
Human Red Cells

Methods have been described for the extraction and purification of an agent inhibiting the hemagglutination of red cells by influenza (PR8) and mumps viruses. Human red cells have served as the chief source of the inhibitor but the latter has also been found in human lung. The active extracts have been purified to the extent that 0.1 gamma of material suffices to inhibit one hemagglutinating dose of virus. Incomplete chemical characterization of the most highly purified fractions available indicates the presence of 2.6 per cent nitrogen, at least 50 per cent of polysaccharide, and no phosphorus. In the ultracentrifuge the purified preparation behaves as a polydisperse macromolecular substance. The active material can be obtained from red cell stroma in an ether- and chloroform-soluble form which, on further treatment, can be converted into chloroform-insoluble material. It is possible that the former represents more closely the virus receptor as it exists in the red cell. The purified inhibitor is inactivated on incubation with the virus at 37°C. The nature of this effect is being investigated.

Preparation and Characterization of Molecular Imprinted Polymers of Tetracycline Antibiotics by Mixed-Templates

2011 ◽  
Vol 128-129 ◽  
pp. 407-410
Author(s):  
Guo Li Gong ◽  
Lin Jia ◽  
Hui Li ◽  
Xiang Jun Qi
Keyword(s):  
Functional Monomer ◽  
Molecular Imprinted Polymers ◽  
Tetracycline Antibiotics ◽  
Imprinted Polymers ◽  
Extraction And Purification ◽  
Bonding Property ◽  
Cross Linker ◽  
Selection For ◽  
High Selection

Molecular imprinted polymers (MIPs) were synthesized in certain conditions in the experiment when tetracycline antibiotics (TCs), including Oxytetracycline, Chlortetracycline and Tetracycline were used as templates, methacrylic acid as functional monomer, and ethylene glycol dimethacrylate as cross-linker. Oxytetracycline and Chlortetracycline (1:1) were chosen as mixed-templates when the bonding property of the polymer was studied. The results showed that the mixed-templates MIPs had high selection for tetracycline, and were expected to be used as separation materials for the extraction and purification of TCs in animal food.

Purification and Characterization of Limit Dextrinase from Malted Barley

2012 ◽  
Vol 550-553 ◽  
pp. 1747-1754
Author(s):  
Ya Li Peng ◽  
Fei Hu
Keyword(s):  
Metal Ion ◽  
Ph Value ◽  
Maximum Activity ◽  
Effect Of Temperature ◽  
Purification And Characterization ◽  
Crude Extracts ◽  
Extraction And Purification ◽  
Sds Page ◽  
Limit Dextrinase

Limit dextrinase is one of three main amylases in malted barley, which plays a significant role during the mashing stage of brewing. Due to very low content and similar properties compared to other amylases in malted barley, limit dextrinase is hard to separate effectively. Our work had been directed towards the extraction and purification of limit dextrinase from malted barley. Final products were obtained through fraction precipitation with ammonium sulfate and column chromatography, and purified limit dextrinase acquired a high purity of 31.23 times as much as that of crude extracts. The previous results were also confirmed by sodiumdodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE) revealing a single band of protein (~97KDa). Effect of temperature, pH value, and metal ion on hydrolysis characterization of limit dextrinase was investigated. The results indicated that the maximum activity of purified samples changed significantly compared with that of crude extracts. The activity of purified limit dextrinase could be activated by lower concentration of Mg2+、Ca2+、Mn2+ and inhibited by the action of Zn2+、Fe2+. But this influence was not so obvious for K+.

Extraction and Purification of Isoflavones from Soybeans and Characterization of Their Estrogenic Activities

2007 ◽  
Vol 55 (17) ◽  
pp. 6940-6950 ◽  
Author(s):  
Elizabeth Jingnan Zhang ◽  
Ka Ming Ng ◽  
Kathy Qian Luo
Keyword(s):  
Extraction And Purification

Optimized DNA Extraction and Purification Method for Characterization of Bacterial and Fungal Communities in Lung Tissue Samples

2019 ◽  
Author(s):  
Vicente Pérez-Brocal ◽  
Fabien Magne ◽  
Susana Ruiz-Ruiz ◽  
Carolina A. Ponce ◽  
Rebeca Bustamante ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Lung Tissue ◽  
Fungal Communities ◽  
Purification Method ◽  
Tissue Samples ◽  
Extraction And Purification

scholarly journals The Enrichment And Characterization Of Compost And Wastewater-Derived Microbial Cellulolytic Consortia For Biofuel Production

2021 ◽  
Author(s):  
Augustyna Dobosz
Keyword(s):  
Energy Demand ◽  
Defined Medium ◽  
Biofuel Production ◽  
Microbial Consortia ◽  
Extraction And Purification ◽  
End Products ◽  
Fermentative Activity ◽  
Incubation Temperatures ◽  
Current Energy

Over the last decade, a rise in energy demand and diminishing fuel resources have created a challenge for finding an alternative solution that could supplement our current energy sources. This study demonstrated that ethanol and other useful end-products can be produced from the fermentative activity of microbial consortia derived from cellulose-rich waste environments. Compost and wastewater were used as inoculum sources to enrich cellulolytic cultures at incubation temperatures 50 ºC and 60ºC. A chemically defined medium was used without complex nutrients such as yeast extract. Four cellulolytic cultures were obtained and their end-products were monitored over an active cellulose degrading period. The compost culture incubated at 50ºC produced the highest concentration of butyrate while the wastewater-derived culture incubated at 60ºC produced the highest ethanol concentration. Optimization of DNA extraction and purification from complex environmental samples such as the compost and wastewater cultures used in this study was also discussed.

scholarly journals Production and Characterization of Immunoglobuline Yolk as anti antigen membrane Toxoplasma gondii

2016 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
Heni Puspitasari ◽  
Yuliana Praptiwi ◽  
Lucia Suwanti
Keyword(s):  
Molecular Weight ◽  
Toxoplasma Gondii ◽  
Egg Yolk ◽  
Immunoglobulin Y ◽  
Extraction And Purification ◽  
Sds Page ◽  
Protein Membrane ◽  
Peritoneal Infection ◽  
Antigen Epitope

Toxoplasma gondii is an obligate parasite intracellular which can infected human and other mammalian. Immunoglobulin Y technology  offers several  advantages better than antibody production in mammals. This research was aimed to get immunoglobulin Y from egg yolk, to prove that antibody against membrane T. gondii antigen can produced from immunoglobulin Y and to know the characterization of  immunoglobuline Y according to  molecular weight by SDS PAGE and reactivation of  antibody antigen  by Western Blott. This research devided  from many step : passase tachyzoites T. gondii into mice by peritoneal infection, cultivated  the tachyzoite from intraperitoneal fluid, preparation of  membrane antigen tachyzoite T. gondii, then  immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blott.  The result of this resarch showed that immunoglobulin Y from egg yolk can  produced antibody against protein membrane T. gondii. The result of analyzed profile protein immunoglobuline  Y according SDS PAGE  has molecular weight 179,8 kDa. Analyzed from Western Blott showed that immunoglobulin Y can recognize antigen epitope of  T. gondii on molecular weight 35,7 kDa and 78,8 kDa.

Characterization of intracellular polysaccharides of Streptomyces

1982 ◽  
Vol 28 (12) ◽  
pp. 1320-1323 ◽  
Author(s):  
Alfredo F. Braña ◽  
Manuel B. Manzanal ◽  
Carlos Hardisson
Keyword(s):  
Structural Properties ◽  
Macromolecular Structure ◽  
Degree Of Branching ◽  
Extraction And Purification ◽  
Enzymatic Methods

The intracytoplasmic accumulation of polysaccharides has been demonstrated in four species of Streptomyces. After extraction and purification, the structural properties of these polysaccharides were investigated by chemical, spectrophotometric, and enzymatic methods. The results clearly indicated that the streptomycete polysaccharides have a degree of branching and a macromolecular structure similar to that of the glycogens isolated from other bacteria and animal sources.

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