Inhibitory Effect of Phlorotannins Isolated from Ecklonia cava on Mushroom Tyrosinase Activity and Melanin Formation in Mouse B16F10 Melanoma Cells

2009 ◽  
Vol 57 (10) ◽  
pp. 4124-4129 ◽  
Author(s):  
Na Young Yoon ◽  
Tae-Kil Eom ◽  
Moon-Moo Kim ◽  
Se-Kwon Kim
Keyword(s):  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Ecklonia Cava ◽  
Mushroom Tyrosinase ◽  
B16f10 Melanoma ◽  
Melanin Formation ◽  
B16f10 Melanoma Cells

An In-Vitro Assay Estimating Changes in Melanin Content of Melanoma Cells due to Ultra-Dilute, Potentized Preparations

Homeopathy ◽  
2019 ◽  
Vol 108 (03) ◽  
pp. 183-187 ◽  
Author(s):  
Renuka Munshi ◽  
Samidha Joshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
In Vitro Study ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16f10 Melanoma ◽  
Vitro Assay ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Physiological Dose

Introduction The authors had previously conducted an in-vitro study to observe the effect of homeopathic medicines on melanogenesis, demonstrating anti-vitiligo potential by increasing the melanin content in murine B16F10 melanoma cells. A similar experiment was performed using further homeopathic preparations sourced from kojic acid (KA), hydrogen peroxide (H2O2; HP), 6-biopterin (BP), and [Nle4, D-Phe7]-α-melanocyte-stimulating hormone (NLE), some of which are known to induce vitiligo or melano-destruction at physiological dose. Materials and Methods The homeopathic preparations of BP, KA, NLE, and HP were used in 30c potency. Alcohol and potentized alcohol were used as vehicle controls. Prior to starting the main experiment, the viability of B16F10 melanoma cells after treatment with study preparations was assayed. Melanin content (at 48 h and 96 h) and tyrosinase activity in melanocytes were determined. Results At the end of 48 hours, NLE and HP in 30c potency had a significantly greater melanin content (p = 0.015 and p = 0.039, respectively) compared with controls; BP and KA in 30c potency had no significant effects. No significant changes were seen at the end of 96 hours. KA, NLE, HP, and vehicle controls showed an inhibition of tyrosinase activity. Conclusion The study demonstrated melanogenic effects of two homeopathic preparations. Further research to evaluate the therapeutic efficacy of these medicines is warranted.

scholarly journals Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition byN-Acetyl-pentapeptides

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ching-Yi Lien ◽  
Ching-Yu Chen ◽  
Shih-Ting Lai ◽  
Chin-Feng Chan
Keyword(s):  
Kojic Acid ◽  
Lag Time ◽  
Melanoma Cells ◽  
Dependent Manner ◽  
Mushroom Tyrosinase ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells ◽  
Kinetics Of ◽  
Dose Dependent

We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation ofL-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC500.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.

Inhibitory Effects of Dryopteris erythrosora Extract on Tyrosinase Activity and Melanin Production in B16F10 Melanoma Cells

KSBB Journal ◽  
2020 ◽  
Vol 35 (3) ◽  
pp. 228-234
Author(s):  
Yeon-Su Koo ◽  
Taejin Park ◽  
Ji Han Sim ◽  
Min-Seon Kim ◽  
Seung-Young Kim
Keyword(s):  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
B16f10 Melanoma Cells

Effect of apigenin-7-glucoside, genkwanin and naringenin on tyrosinase activity and melanin synthesis in B16F10 melanoma cells

Life Sciences ◽  
2016 ◽  
Vol 144 ◽  
pp. 80-85 ◽  
Author(s):  
Nouha Nasr Bouzaiene ◽  
Fadwa Chaabane ◽  
Aicha Sassi ◽  
Leila Chekir-Ghedira ◽  
Kamel Ghedira
Keyword(s):  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Synthesis ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Azadirachta indica (Neem) Water Leaf Extract Inhibits Melanin Production and Tyrosinase Activity in B16F10 Melanoma Cells

2021 ◽  
Vol 13 (4) ◽  
pp. 1030-1035
Author(s):  
Thanitsara Songtavisin ◽  
Benjamart Pratoomthai ◽  
Warachin Gangnonngiw ◽  
Jarinyaporn Naowaboot
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
B16f10 Melanoma Cells
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