Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition byN-Acetyl-pentapeptides

We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation ofL-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC500.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.

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Melanogenesis Effect of 7-acetoxy-4-methylcoumarin in B16F10 Melanoma Cells

Cosmetics ◽

10.3390/cosmetics7040094 ◽

2020 ◽

Vol 7 (4) ◽

pp. 94

Author(s):

Ji-Han Sim ◽

Sung-Chan Jang ◽

Tae-Jin Park ◽

Won-Jae Chi ◽

Seung-Young Kim

Keyword(s):

Melanoma Cells ◽

Hair Follicles ◽

Dependent Manner ◽

B16f10 Melanoma ◽

Melanin Production ◽

B16f10 Cells ◽

B16f10 Melanoma Cells ◽

Diphenyl Tetrazolium Bromide ◽

Synthetic Derivatives ◽

Dose Dependent

The increased interest in anti-whitening dyes has enhanced the research interest to identify efficient melanogenic activators. Melanogenesis is the process of melanin production by melanocytes in the hair follicles and skin, which is mediated by several enzymes, such as microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein (TRP)-1, and TRP-2. This study investigated the melanogenesis-stimulating effect of 4-Methylumbelliferone (4MUMB) and its synthetic derivatives, 7-acetoxy-4-methylcoumarin (7A4MC) and 4-methylheriniarin (4MH) in B16F10 melanoma cells. The cytotoxicity of these compounds was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, followed by the assessment of the melanin content and the intracellular TYR activity. Finally, the expression levels of the key enzymes involved in melanogenesis were investigated. 7A4MC increased melanin production in B16F10 cells relative to that by 4MUMB and 4MH treated cells in a dose-dependent manner without significant cytotoxicity. Concomitantly, 7A4MC significantly increased TYR activity and enhanced the expression of MITF, which significantly induced the expression of TRP-1, TRP-2, and TYR. Furthermore, 7A4MC stimulated melanogenesis via increased phosphorylation of c-Jun N-terminal kinases (JNK) and reduced phosphorylation of protein kinase B (AKT). These results confirmed the melanogenesis-inducing effects of 7A4MC and indicated its potential use as an anti-hair bleaching agent in cosmetics industries.

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Inhibitory Effects of Myelophycus simplex Papenfuss Methanol Extract on Melanogenesis in B16F10 Melanoma Cells

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2017.46.1.034 ◽

2017 ◽

Vol 46 (1) ◽

pp. 34-38

Author(s):

Hyang Suk Kim ◽

Ji Min Cheon ◽

Da Hye Kwon ◽

Eun Ok Choi ◽

Min Ju Kim ◽

...

Keyword(s):

Methanol Extract ◽

Melanoma Cells ◽

Inhibitory Effects ◽

B16f10 Melanoma ◽

B16f10 Melanoma Cells

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Mushroom Tyrosinase Inhibition and Antimelanogenesis Activities of <i>Bacopa monnieri</i> (L.) Methanol Extract in B16F10 Melanoma Cells

Journal of Biosciences and Medicines ◽

10.4236/jbm.2020.85018 ◽

2020 ◽

Vol 08 (05) ◽

pp. 189-202

Author(s):

T. N. Shamu ◽

Junna Lalitha ◽

Manohar Shinde

Keyword(s):

Methanol Extract ◽

Melanoma Cells ◽

Bacopa Monnieri ◽

Mushroom Tyrosinase ◽

B16f10 Melanoma ◽

Tyrosinase Inhibition ◽

B16f10 Melanoma Cells

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The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

Natural Product Communications ◽

10.1177/1934578x1400900433 ◽

2014 ◽

Vol 9 (4) ◽

pp. 1934578X1400900

Author(s):

Prasit Sirwannalert ◽

Ryusho Kariya ◽

Ikuko Suzu ◽

Seiji Okada

Keyword(s):

Melanoma Cells ◽

Pharmaceutical Products ◽

B16 Melanoma ◽

Tyrosinase Activity ◽

Root Extract ◽

Dependent Manner ◽

Inhibitory Effects ◽

B16 Melanoma Cells ◽

Dose Dependent ◽

Salacia Reticulata

The purposes of this study were to investigate the inhibitory effects of Salacia reticulata Tul. root extract on cellular oxidants and melanogenesis in B16 melanoma cells. Cells treated with non-toxic doses of S. reticulata root extract were investigated for their effects on melanogenesis, cellular tyrosinase activity and cellular oxidant scavenging activity. The results indicated that S. reticulata extract inhibited melanin synthesis and tyrosinase activity in α-MSH-induced or UV-irradiated B16 melanoma cells in a dose dependent manner. Additionally, the extract also exhibited anti-cellular oxidants in UV-induced radical melanoma cells. Altogether, these results suggested that S. reticulata root extract has roles in suppression of melanogenesis and oxidant inhibition. S. reticulata root extract may be a potential source for the development of pharmaceutical products for treatment of skin hyperpigmentation disorders.

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Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin

Phytomedicine ◽

10.1016/j.phymed.2013.04.015 ◽

2013 ◽

Vol 20 (12) ◽

pp. 1082-1087 ◽

Cited By ~ 38

Author(s):

Li-Hua Peng ◽

Shuai Liu ◽

Shen-Yao Xu ◽

Lei Chen ◽

Ying-Hui Shan ◽

...

Keyword(s):

Guinea Pig ◽

Melanoma Cells ◽

Tyrosinase Activity ◽

Ultraviolet B ◽

Melanin Synthesis ◽

Inhibitory Effects ◽

B16f10 Melanoma ◽

Pig Skin ◽

B16f10 Melanoma Cells

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Anti-oxidant Function and Inhibitory Effects of the Expression of MITF, TRP-1, TRP-2 and Tyrosinase of Sesamum indicum L. in B16F10 Melanoma Cells

Journal of Life Science ◽

10.5352/jls.2017.27.3.318 ◽

2017 ◽

Vol 27 (3) ◽

pp. 318-324

Author(s):

Dan-Hee Yoo ◽

Da-Hye Joo ◽

Jin-Young Lee

Keyword(s):

Melanoma Cells ◽

Sesamum Indicum ◽

Inhibitory Effects ◽

B16f10 Melanoma ◽

B16f10 Melanoma Cells ◽

Anti Oxidant

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Evaluate the Cytotoxicity of Kojic Acid Nanocomposites on Melanoma Cells and Normal Cells of the Skin

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.36.45 ◽

2018 ◽

Vol 36 ◽

pp. 45-55 ◽

Cited By ~ 3

Author(s):

Samer Hasan Hussein-Al-Ali ◽

Palanisamy Arulselvan ◽

Mohd Zobir Hussein ◽

Sharida Fakurazi ◽

Bullo Saifullah

Keyword(s):

Skin Cancer ◽

Cancer Cells ◽

Kojic Acid ◽

Normal Skin ◽

Melanoma Cells ◽

Dermal Fibroblasts ◽

Dependent Manner ◽

Human Dermal Fibroblasts ◽

Skin Cell ◽

Dose Dependent

Iron oxide nanoparticles (MNPs) was synthesized by coprecipitation of Fe+2and Fe+3into highly basic media, followed by coating with chitosan (CH) and polyethylene glycol (PG) to forming CH-MNPs and PG-MNPs nanoparticles, respectively. Kojic acid (Kj) drug was loaded on the CH-MNPs and PG-MNPs nanoparticles to forming Kj-CH-MNPs and Kj-PG-MNPs nanocomposites. The potential cytotoxicity of free Kj, MNPs, Kj-CH-MNPs and Kj-PG-MNPs nanocomposites was evaluated using skin cancer cells (B16-F10 melanoma cells) and normal skin cell (Human Dermal Fibroblasts murine). Kj at concentrations in the range 1.562–50 μg/mL did not affect on the viability of normal skin cell and skin cancer cells during a 72-hours incubation. The Kj-CH-MNPs and Kj-PG-MNPs nanocomposites exhibit significant cytotoxicity in skin cancer cells in a dose-dependent manner with an IC50value 47.1 and 8.4 μg/mL, respectively.

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