Dynamic Light Scattering Investigation in Aqueous Solutions of bc1-Complex Membrane Protein

2002 ◽  
Vol 106 (16) ◽  
pp. 4318-4324 ◽  
Author(s):  
Kazuo Onuma ◽  
Tomomi Kubota ◽  
Shinpei Tanaka ◽  
Noriko Kanzaki ◽  
Atsuo Ito ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Membrane Protein ◽  
Aqueous Solutions ◽  
Bc1 Complex ◽  
Complex Membrane

scholarly journals COMPLEXATION OF POLYETHYLENE-GLYCOL WITH THE SODIUM SALTS OF CITRIC AND SUCCINIC ACIDS IN THE AQUEOUS SOLUTIONS. STUDIES BY DYNAMIC LIGHT SCATTERING AND UV/VIS SPECTROPHOTOMETRY

2015 ◽  
Vol 11 (8) ◽  
pp. 3866-3872
Author(s):  
E.A. Masimov ◽  
Etibar Hummat Ismailov ◽  
S.Y. Odzhaqverdiyeva
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Polyethylene Glycols ◽  
Hydrodynamic Diameter ◽  
Absorption Bands ◽  
Sodium Salts ◽  
And Diffusion ◽  
Formation Of Complexes

Dynamic light scattering (DLS) method in combination with the UV/VIS spectrophotometry is used to study the interaction of polyethylene- glycols with a molecular weight  6000 ( PEG6000 ) with sodium salts of citric and succinic acids in aqueous solutions. The values of density, viscosity, refractive and diffusion indexes, the values of the hydrodynamic diameter, wavelength electronic absorption bands for PEG6000 aqueous solutions, their mixtures with succinic and citric acids are determined. It was shown that depending on the composition of the solutions the values of hydrodynamic diameter for aqueous solutions containing 1-5 wt.% PEG6000 and their mixtures with succinic and citric acids (~ 1 wt%) ranges from 3.6 to 5.2 nm. It is assumed that the formation of complexes with the sizes  that are within the above range is due to the features of interaction  and the structure of the complexes formed in solution.

scholarly journals PECULARITIES OF MICELLE FORMATION OF PENTACYCLIC GLYCOSIDES IN AQUEOUS SOLUTIONS

2021 ◽  
Vol 64 (4) ◽  
pp. 26-33
Author(s):  
Natalia V. Mironenko ◽  
Irina V. Shkutina ◽  
Vladimir F. Selemenev
Keyword(s):  
Surface Tension ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Hydrodynamic Radius ◽  
Critical Concentration ◽  
Micelle Formation ◽  
Cylindrical Shape ◽  
Scattering Method ◽  
Dynamic Light Scattering Method

The regularities of changes in structural characteristics during the formation of associates in micellar aqueous solutions of triterpene saponins Quillaja Saponin and Sapindus Mukorossi are considered. The dependence of surface tension and adsorption on the concentration of an aqueous saponin solution is analyzed, and the values of surface activity and parameters of the adsorption layer are calculated. The average values of diffusion coefficients for spherical and cylindrical micelles are determined based on the measurement of the solution viscosity. The effect of the electrolyte solution on the surface tension and viscosity of glycoside solutions is studied: when the electrolyte is introduced into the saponin solution, the surface tension decreases, which leads to a shift in the critical concentration of micelle formation towards lower concentrations. The introduction of potassium chloride electrolyte reduces the degree of ionization and, as a result of suppressing the electroviscosity effect, leads to a decrease in the viscosity of the solution. The dynamic light scattering method is used to determine the size of glycoside aggregates. It is established that there are aggregates of several sizes in an aqueous solution of saponin. The size and shape of aggregates were calculated using the concepts of micelle packing parameters. In the region of very low concentrations of glycoside solutions, when approaching the critical concentration of micelle formation in the solution, there are spherical micelles. A further increase in the saponin concentration in the solution leads to a decrease in the content of structures with a hydrodynamic radius of 50-80 nm and the appearance of larger agglomerates with sizes greater than 100 nm. It was found that micelles acquire a less hydrated and more densely packed cylindrical shape in the concentration range of 1.7-2.6 mmol/dm3. Compaction of associates leads to an increase in the content of particles with a hydrodynamic radius of 150-250 nm and larger ones, and their presence predicts the appearance of larger agglomerates. Analyzing the data obtained using the dynamic light scattering method, it can be concluded that aggregates of several sizes co-exist in the volume of aqueous saponin solutions at certain concentrations.

Aggregates of alginates binding with surfactants of single and twin alkyl chains in aqueous solutions: Fluorescence and dynamic light scattering studies

2006 ◽  
Vol 66 (2) ◽  
pp. 266-273 ◽  
Author(s):  
Biye Ren ◽  
Yiming Gao ◽  
Lu Lu ◽  
Xinxing Liu ◽  
Zhen Tong
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Alkyl Chains

Dynamic light scattering and rheology studies of aqueous solutions of amphiphilic sodium maleate containing copolymers

2007 ◽  
Vol 45 (7) ◽  
pp. 774-785 ◽  
Author(s):  
Emanuela Di Cola ◽  
Thomas A. Waigh ◽  
Ralph H. Colby
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Sodium Maleate

Characterization of Riboflavin-Photosensitized Changes in Aqueous Solutions of Alginate by Dynamic Light Scattering

2004 ◽  
Vol 4 (2) ◽  
pp. 76-83 ◽  
Author(s):  
Anna-Lena Kjøniksen ◽  
Stefania G. Baldursdóttir ◽  
Bo Nyström
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions

Dynamic Light Scattering Experiments with Aqueous Solutions of a Cascade Molecule: Benzene[9]3-arborol

1993 ◽  
Vol 97 (1) ◽  
pp. 33-35 ◽  
Author(s):  
Th.-P. Engelhardt ◽  
L. Belkoura ◽  
D. Woermann ◽  
W. Grimme
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions

scholarly journals Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

2015 ◽  
Vol 71 (7) ◽  
pp. 838-846 ◽  
Author(s):  
Mohamed Dahani ◽  
Laurie-Anne Barret ◽  
Simon Raynal ◽  
Colette Jungas ◽  
Pétra Pernot ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Membrane Protein ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Surfactant Phase ◽  
Ray Scattering

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

Rheological and dynamic light-scattering studies on aqueous solutions of a hydrophobically modified nonionic cellulose ether and its unmodified analog

Langmuir ◽  
1995 ◽  
Vol 11 (6) ◽  
pp. 1994-2002 ◽  
Author(s):  
Bo Nystroem ◽  
Krister Thuresson ◽  
Bjoern Lindman
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Cellulose Ether ◽  
Hydrophobically Modified

Dynamic Light Scattering and Viscosity Studies on the Association Behavior of Silicone Surfactants in Aqueous Solutions

2003 ◽  
Vol 107 (22) ◽  
pp. 5382-5390 ◽  
Author(s):  
Saurabh S. Soni ◽  
Nandhibatla V. Sastry ◽  
John George ◽  
H. B. Bohidar
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Silicone Surfactants ◽  
Association Behavior
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