Protein Films of Bovine Serum Albumen Conjugated Gold Nanoparticles: A Synthetic Route from Bioconjugated Nanoparticles to Biodegradable Protein Films

2011 ◽  
Vol 115 (7) ◽  
pp. 2982-2992 ◽  
Author(s):  
Mandeep Singh Bakshi ◽  
Harpreet Kaur ◽  
Poonam Khullar ◽  
Tarlok Singh Banipal ◽  
Gurinder Kaur ◽  
...  

2013 ◽  
Vol 15 (11) ◽  
Author(s):  
Yuyang Ni ◽  
Jun Li ◽  
Zhenzhen Huang ◽  
Ke He ◽  
Jiaqi Zhuang ◽  
...  




2016 ◽  
Vol 222 ◽  
pp. 1709-1715 ◽  
Author(s):  
Chuanxin He ◽  
Minsui Xie ◽  
Fei Hong ◽  
Xiaoyan Chai ◽  
Hongwei Mi ◽  
...  




Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3395 ◽  
Author(s):  
Iulia Matei ◽  
Cristina Maria Buta ◽  
Ioana Maria Turcu ◽  
Daniela Culita ◽  
Cornel Munteanu ◽  
...  

The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution of bovine serum albumin (BSA) at room temperature. The processes were monitored by UV-Vis, circular dichroism, Raman and electron paramagnetic resonance (EPR) spectroscopies. TEM microscopy and dynamic light scattering (DLS) measurements were used to evidence changes in particle size during nanoparticle formation and growth. The formation of AuNPs at pH 7 in the absence of BSA was not observed, which proves that the albumin is involved in the first step of Au(III) reduction. Changes in the EPR spectral features of two spin probes, CAT16 and DIS3, with affinity for BSA and AuNPs, respectively, allowed us to monitor the particle growth and to demonstrate the protective role of BSA for AuNPs. The size of AuNPs formed in BSA solution increases slowly with time, resulting in nanoparticles of different morphologies, as revealed by TEM. Raman spectra of BSA indicate the interaction of albumin with AuNPs through sulfur-containing amino acid residues. This study shows that albumins act as both reducing agents and protective corona of AuNPs.



2019 ◽  
Vol 38 (15) ◽  
pp. 4644-4654 ◽  
Author(s):  
Bahareh Khodashenas ◽  
Mehdi Ardjmand ◽  
Mazyar Sharifzadeh Baei ◽  
Ali Shokuhi Rad ◽  
Azim Akbarzadeh Khiyavi


Langmuir ◽  
2009 ◽  
Vol 25 (20) ◽  
pp. 12229-12234 ◽  
Author(s):  
Takeshi Serizawa ◽  
Yu Hirai ◽  
Mamoru Aizawa


Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4269 ◽  
Author(s):  
Ewa Paradowska ◽  
Katarzyna Arkusz ◽  
Dorota G. Pijanowska

The increasing interest of attachment of gold nanoparticles (AuNPs) on titanium dioxide nanotubes (TNTs) has been devoted to obtaining tremendous properties suitable for biosensor applications. Achieving precise control of the attachment and shape of AuNPs by methods described in the literature are far from satisfactory. This work shows the comparison of physical adsorption (PA), cyclic voltammetry (CV) and chronoamperometry (CA) methods and the parameters of these methods on TNTs properties. The structural, chemical, phase and electrochemical characterizations of TNTs, Au/TNTs, AuNPs/TNTs are carried out using scanning electron microscopy (SEM), electrochemical impedance spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy. The use of PA methods does not allow the deposition of AuNPs on TNTs. CV allows easily obtaining spherical nanoparticles, for which the diameter increases from 20.3 ± 2.9 nm to 182.3 ± 51.7 nm as a concentration of tetrachloroauric acid solution increase from 0.1 mM to 10 mM. Increasing the AuNPs deposition time in the CA method increases the amount of gold, but the AuNPs diameter does not change (35.0 ± 5 nm). Importantly, the CA method also causes the dissolution of the nanotubes layer from 1000 ± 10.0 nm to 823 ± 15.3 nm. Modification of titanium dioxide nanotubes with gold nanoparticles improved the electron transfer and increased the corrosion resistance, as well as promoted the protein adsorption. Importantly, after the deposition of bovine serum albumin, an almost 5.5-fold (324%) increase in real impedance, compared to TNTs (59%) was observed. We found that the Au nanoparticles—especially those with smaller diameter—promoted the stability of bovine serum albumin binding to the TNTs platform. It confirms that the modification of TNTs with gold nanoparticles allows the development of the best platform for biosensing applications.



Author(s):  
A. B. Yule ◽  
G. Walker

The tenacity of the barnacle cyprids of Balanus balanoides (L.) during temporary adhesion to a variety of modified Perspex surfaces was tested using a sensitive microbalance. The Perspex panels were painted on the undersides with five colours of cellulose paint. Control measurements of the magnitude of temporary adhesion to clean slate panels were of the order of 2 × 10 N m. Untreated smooth Perspex panels and panels coated with an adsorbed layer of bovine serum albumen gave low figures of tenacity of the order of 0·8 × 10 N m. Roughening the panels with carborundum or treating with an adsorbed layer of barnacle extract protein resulted in higher tenacity measurements (1·0–1·8 × 10 N M), whereas the highest measurements were obtained when roughened Perspex panels were coated by an adsorbed layer of barnacle extract protein (1·6–2·2 × 10 N M). When the surfaces were made more attractive to barnacle cyprids by roughening or coating in barnacle protein the forces measured on dark coloured panels were higher than on lighter coloured panels. The results of the temporary adhesion measurements were closely paralleled by the numbers of cyprids found exploring the various surfaces during preference settlement experiments.



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