Does the Reconstitution of RC-LH1 Complexes from Rhodopseudomonas acidophila Strain 10050 into a Phospholipid Bilayer Yield the Optimum Environment for Optical Spectroscopy?

2013 ◽  
Vol 117 (48) ◽  
pp. 15004-15013 ◽  
Author(s):  
Paul S. Böhm ◽  
Ralf Kunz ◽  
June Southall ◽  
Richard J. Cogdell ◽  
Jürgen Köhler
Keyword(s):  
Optical Spectroscopy ◽  
Phospholipid Bilayer ◽  
Rhodopseudomonas Acidophila

Factors Affecting the lnteraction of Tissue Factor/Factor Vll with Factor X in a Heterogeneous Tubular Reactor

1991 ◽  
Vol 65 (02) ◽  
pp. 139-143 ◽  
Author(s):  
Cynthia H Gemmell ◽  
Vincet T Turitto ◽  
Yale Nemerson
Keyword(s):  
Steady State ◽  
Tissue Factor ◽  
Factor Viia ◽  
Transmembrane Protein ◽  
Strong Association ◽  
Tubular Reactor ◽  
Factor Xa ◽  
Phospholipid Bilayer ◽  
Factor Vii ◽  
Factor X

SummaryA novel reactor recently described for studying phospholipiddependent blood coagulation reactions under flow conditions similar to those occurring in the vasculature has been further charactenzed. The reactor is a capitlary whose inner wall is coated with a stable phospholipid bilayer (or two bilayers) containing tissue factor, a transmembrane protein that is required for the enzymatic activation of factor X by factor VIIa. Perfusion of the capillary at wall shear rates ranging from 25 s−1 to 1,200 s−1 with purified bovine factors X and VIIa led to steady state factor Xa levels at the outlet. Assay were performed using a chromogenic substrate, SpectrozymeTMFXa, or by using a radiometric technique. In the absence of Ca2+ or factor VIIa there was no product formation. No difference was noted in the levels of factor Xa achieved when non-activated factor VII was perfused. Once steady state was achieved further factor Xa production continued in the absence of factor VIIa implying a very strong association of factor VIIa with the tissue factor in the phospholipid membrane. In agreement with static vesicle-type studies the reactor was sensitive to wall tissue factor concentration, temperature and the presence of phosphatidylserine in the bilayer.

Using optical spectroscopy for the study of paper degradation

2006 ◽  
Vol 49 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Mauro Missori ◽  
Marcofabio Righini
Keyword(s):  
Optical Spectroscopy ◽  
Paper Degradation

Properties of Ultraviolet Exposed Camptothecin Studied by Using Optical Spectroscopy Methods

2016 ◽  
Vol 19 (4) ◽  
pp. 319-324 ◽  
Author(s):  
Blanka Ziomkowska ◽  
Tomasz Wybranowski ◽  
Michal Cyrankiewicz ◽  
Stefan Kruszewski
Keyword(s):  
Optical Spectroscopy

Intraspecific differentiation of sandflies specimens by optical spectroscopy and multivariate analysis

2021 ◽  
Author(s):  
Aline E. Casaril ◽  
Carlos G. Santos ◽  
Bruno S. Marangoni ◽  
Sandro M. Lima ◽  
Luis H.C. Andrade ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Optical Spectroscopy ◽  
Intraspecific Differentiation

Growth of pentacene on α−Al2O3 (0001) studied by in situ optical spectroscopy

2017 ◽  
Vol 1 (4) ◽  
Author(s):  
Lei Zhang ◽  
X. Fu ◽  
M. Hohage ◽  
P. Zeppenfeld ◽  
L. D. Sun
Keyword(s):  
Optical Spectroscopy ◽  
Α Al2o3

Accurate determination of the onset wavelength (λonset) in optical spectroscopy

2021 ◽  
Vol 265 ◽  
pp. 107544
Author(s):  
Austin M. Wallace ◽  
Christine Curiac ◽  
Jared H. Delcamp ◽  
Ryan C. Fortenberry
Keyword(s):  
Optical Spectroscopy ◽  
Accurate Determination

scholarly journals Evaluation of the correlation between porphyrin accumulation in cancer cells and functional positions for application as a drug carrier

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Koshi Nishida ◽  
Toshifumi Tojo ◽  
Takeshi Kondo ◽  
Makoto Yuasa
Keyword(s):  
Cancer Cells ◽  
Drug Carrier ◽  
Bilayer Membrane ◽  
Phospholipid Bilayer ◽  
Membrane Permeation ◽  
Porphyrin Derivatives ◽  
Phospholipid Bilayer Membrane ◽  
Meso Position ◽  
Tumor Accumulation ◽  
Binding Position

AbstractPorphyrin derivatives accumulate selectively in cancer cells and are can be used as carriers of drugs. Until now, the substituents that bind to porphyrins (mainly at the meso-position) have been actively investigated, but the effect of the functional porphyrin positions (β-, meso-position) on tumor accumulation has not been investigated. Therefore, we investigated the correlation between the functional position of substituents and the accumulation of porphyrins in cancer cells using cancer cells. We found that the meso-derivative showed higher accumulation in cancer cells than the β-derivative, and porphyrins with less bulky substituent actively accumulate in cancer cells. When evaluating the intracellular distribution of porphyrin, we found that porphyrin was internalized by endocytosis and direct membrane permeation. As factors involved in these two permeation mechanisms, we evaluated the affinity between porphyrin-protein (endocytosis) and the permeability to the phospholipid bilayer membrane (direct membrane permeation). We found that the binding position of porphyrin affects the factors involved in the transmembrane permeation mechanisms and impacts the accumulation in cancer cells.

scholarly journals Optical spectroscopy of excited exciton states in MoS2 monolayers in van der Waals heterostructures

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
C. Robert ◽  
M. A. Semina ◽  
F. Cadiz ◽  
M. Manca ◽  
E. Courtade ◽  
...  
Keyword(s):  
Optical Spectroscopy ◽  
Van Der Waals ◽  
Van Der Waals Heterostructures ◽  
Mos2 Monolayers
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