Real-Time in Situ Monitoring of Optical Absorption Changes in Visible-Light-Active TiO2 under Light Irradiation and Temperature-Programmed Annealing

AbstractBiocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.

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Real‐time in situ monitoring of poly(lactide‐ co ‐glycolide) coating of coronary stents using electrochemical impedance spectroscopy

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.33250 ◽

2014 ◽

Vol 103 (3) ◽

pp. 691-699 ◽

Cited By ~ 3

Author(s):

Qi Zhong ◽

Qunlong Mao ◽

Jin Yan ◽

Wenming Liu ◽

Tao Zhang ◽

...

Keyword(s):

Electrochemical Impedance Spectroscopy ◽

Impedance Spectroscopy ◽

Real Time ◽

Electrochemical Impedance ◽

Coronary Stents ◽

In Situ Monitoring

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In-situ growth of NiS co-catalyst on MnS/Mn0.3Cd0.7S heterojunction for boosting photocatalytic hydrogen evolution under visible light irradiation

Surfaces and Interfaces ◽

10.1016/j.surfin.2021.101401 ◽

2021 ◽

pp. 101401

Author(s):

Chenhe Shi ◽

Juan Chen ◽

Kaiyu Li ◽

Yanhui Ao

Keyword(s):

Visible Light ◽

Hydrogen Evolution ◽

Visible Light Irradiation ◽

Light Irradiation ◽

In Situ Growth ◽

Co Catalyst ◽

Photocatalytic Hydrogen Evolution

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Construction of Z-Scheme Bi3TaO7/Zn0.5Cd0.5S composites with high-efficiency for levofloxacin degradation under visible light irradiation

Dalton Transactions ◽

10.1039/d1dt02539b ◽

2021 ◽

Author(s):

Jingjing Xu ◽

Yang liu ◽

xueping li ◽

Mindong Chen

Keyword(s):

Visible Light ◽

Hydrothermal Method ◽

Visible Light Irradiation ◽

High Efficiency ◽

Light Irradiation ◽

In Situ Growth ◽

Composite Photocatalysts ◽

Photocatalytic Activities

Direct Z-scheme Bi3TaO7/Zn0.5Cd0.5S composite photocatalysts were successfully prepared via an in-situ growth hydrothermal method. The photocatalytic activities of composites were investigated by the degradation of levofloxacin under visible light. And...

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Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

Applied and Environmental Microbiology ◽

10.1128/aem.68.11.5737-5740.2002 ◽

2002 ◽

Vol 68 (11) ◽

pp. 5737-5740 ◽

Cited By ~ 20

Author(s):

Ariel Maoz ◽

Ralf Mayr ◽

Geraldine Bresolin ◽

Klaus Neuhaus ◽

Kevin P. Francis ◽

...

Keyword(s):

Real Time ◽

Yersinia Enterocolitica ◽

Photon Emission ◽

Photon Counting ◽

In Situ Monitoring ◽

Charge Coupled Device ◽

Reading Frame ◽

Intensified Charge Coupled Device ◽

Camembert Cheese

ABSTRACT Bioluminescent mutants of Yersinia enterocolitica were generated by transposon mutagenesis using a promoterless, complete lux operon (luxCDABE) derived from Photorhabdus luminescens, and their production of light in the cheese environment was monitored. Mutant B94, which had the lux cassette inserted into an open reading frame of unknown function was used for direct monitoring of Y. enterocolitica cells on cheeses stored at 10°C by quantifying bioluminescence using a photon-counting, intensified charge-coupled device camera. The detection limit on cheese was 200 CFU/cm2. Bioluminescence of the reporter mutant was significantly regulated by its environment (NaCl, temperature, and cheese), as well as by growth phase, via the promoter the lux operon had acquired upon transposition. At low temperatures, mutant B94 did not exhibit the often-reported decrease of photon emission in older cells. It was not necessary to include either antibiotics or aldehyde in the food matrix in order to gain quantitative, reproducible bioluminescence data. As far as we know, this is the first time a pathogen has been monitored in situ, in real time, in a “real-product” status, and at a low temperature.

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