Correction to Potent Antifouling Resorcylic Acid Lactones from the Gorgonian-Derived Fungus Cochliobolus lunatus

The development of a reliable procedure for the synthesis of the 16-glucoside and 16-sulfate of the resorcylic acid lactone (RAL) type compound zearalenone is presented. Different protective group strategies were considered and applied to enable the preparation of glucosides and sulfates that are difficult to access up to now. Acetyl andp-methoxybenzyl protection led to undesired results and were shown to be inappropriate. Finally, triisopropylsilyl-protected zearalenone was successfully used as intermediate for the first synthesis of the corresponding mycotoxin glucoside and sulfate that are highly valuable as reference materials for further studies in the emerging field of masked mycotoxins. Furthermore, high stability was observed for aryl sulfates prepared as tetrabutylammonium salts. Overall, these findings should be applicable for the synthesis of similar RAL type and natural product conjugates.

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Carbonyl Reducing 17β-Hydroxysteroid Dehydrogenase from the Filamentous Fungus Cochliobolus lunatus

Advances in Experimental Medicine and Biology - Enzymology and Molecular Biology of Carbonyl Metabolism 6 ◽

10.1007/978-1-4615-5871-2_65 ◽

1996 ◽

pp. 569-577 ◽

Cited By ~ 1

Author(s):

Tea Lanišnik Rižner ◽

Zorko Matjaž ◽

Jasna Peter-Katalinić ◽

Kerstin Strupat ◽

Marija Žakelj-Mavrič

Keyword(s):

Filamentous Fungus ◽

Hydroxysteroid Dehydrogenase ◽

Cochliobolus Lunatus

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Molecular Editing of Kinase-Targeting Resorcylic Acid Lactones (RAL): Fluoroenone RAL

ChemMedChem ◽

10.1002/cmdc.201000047 ◽

2010 ◽

Vol 5 (5) ◽

pp. 670-673 ◽

Cited By ~ 18

Author(s):

Rajamalleswaramma Jogireddy ◽

Sofia Barluenga ◽

Nicolas Winssinger

Keyword(s):

Resorcylic Acid Lactones

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Mating-type genes control sexual reproduction, conidial germination and virulence in Cochliobolus lunatus

Phytopathology ◽

10.1094/phyto-02-21-0063-r ◽

2021 ◽

Author(s):

Kexin Liu ◽

Jiaqi Jia ◽

Nan Chen ◽

Dandan Fu ◽

Jiaying Sun ◽

...

Keyword(s):

Mating Type ◽

Pathogenic Fungus ◽

Germination Rate ◽

Dry Weight ◽

Single Copy ◽

Conidial Germination ◽

Curvularia Lunata ◽

Wild Type ◽

Cochliobolus Lunatus ◽

Mat Genes

Cochliobolus lunatus (anamorph: Curvularia lunata) is a major pathogenic fungus that causes the Curvularia leaf spot of maize. ClMAT1-1-1 and ClMAT1-2-1, the C. lunatus orthologs of Cochliobolus heterostrophus ChMAT1-1-1 and ChMAT1-2-1, were investigated in the present study to uncover their functions in C. lunatus. Southern blot analysis showed that these mating-type MAT genes exist in the C. lunatus genome as a single copy. ClMAT1-1-1 and ClMAT1-2-1 were knocked out and complemented to generate ΔClmat1-1-1 and ΔClmat1-2-1, ΔClmat1-1-1-C and ΔClmat1-2-1-C, respectively. The mutant strains had defective sexual development and failed to produce pseudothecia. There were no significant differences in growth rate or conidia production between the mutant and wild-type strains. However, the aerial mycelia and mycelial dry weight of ΔClmat1-1-1 and ΔClmat1-2-1 were lower than that of wild type, suggesting that MAT genes affect asexual development. ClMAT genes were involved in the responses to cell wall integrity and osmotic adaptation. ΔClmat1-2-1 had a lower conidial germination rate than the wild-type strain CX-3. The virulence of ΔClmat1-2-1 and ΔClmat1-1-1 was also reduced compared to the wild type. Complementary strains could restore all the phenotypes.

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