Direct Atmospheric Pressure Coupling of Polyacrylamide Gel Electrophoresis to Mass Spectrometry for Rapid Protein Sequence Analysis

Journal of Proteome Research ◽

10.1021/pr034031f ◽

2003 ◽

Vol 2 (6) ◽

pp. 610-617 ◽

Author(s):

Joshua J. Coon ◽

Heather A. Steele ◽

Philip J. Laipis ◽

W. W. Harrison

Keyword(s):

Mass Spectrometry ◽

Sequence Analysis ◽

Protein Sequence ◽

Atmospheric Pressure ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Protein Sequence Analysis

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Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis

Proteomics in Functional Genomics ◽

10.1007/978-3-0348-8458-7_1 ◽

2000 ◽

pp. 1-27 ◽

Author(s):

Ettore Appella ◽

David Arnott ◽

Kazuyasu Sakaguchi ◽

Peter J. Wirth

Keyword(s):

Sequence Analysis ◽

Protein Sequence ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Mass Spectrometric ◽

Polyacrylamide Gel ◽

Two Dimensional ◽

Protein Sequence Analysis

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Analysis of proteins copurifying with the cd4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag based protein detection and evaluation of different solubilization methods

Journal of the American Society for Mass Spectrometry ◽

10.1016/s1044-0305(03)00895-x ◽

2004 ◽

Author(s):

O Bernhard

Keyword(s):

Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Protein Detection ◽

Polyacrylamide Gel ◽

Affinity Tag ◽

One Dimensional

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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and centrifuge blotting: Preparation of polypeptides for amino-terminal sequence analysis

Electrophoresis ◽

10.1002/elps.1150170417 ◽

1996 ◽

Vol 17 (4) ◽

pp. 720-722 ◽

Author(s):

Leonila F. Hermansen ◽

Jessie Juul ◽

Knut Sletten

Keyword(s):

Sequence Analysis ◽

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Terminal Sequence ◽

Amino Terminal ◽

Dodecyl Sulfate ◽

Amino Terminal Sequence

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Cross-species identification of novelCandida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Electrophoresis ◽

10.1002/1522-2683(20000701)21:13<2651::aid-elps2651>3.0.co;2-3 ◽

2000 ◽

Vol 21 (13) ◽

pp. 2651-2659 ◽

Author(s):

Mercedes Pardo ◽

Malcolm Ward ◽

Aida Pitarch ◽

Miguel Sánchez ◽

César Nombela ◽

...

Keyword(s):

Mass Spectrometry ◽

Species Identification ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Two Dimensional ◽

Immunogenic Proteins

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Application of both high-performance liquid chromatography combined with tandem mass spectrometry shotgun and 2-D polyacrylamide gel electrophoresis for streptococcal exoproteins gave reliable proteomic data

Microbiology and Immunology ◽

10.1111/j.1348-0421.2010.00302.x ◽

2011 ◽

Vol 55 (2) ◽

pp. 84-94 ◽

Author(s):

Akira Okamoto ◽

Tadao Hasegawa ◽

Keiko Yamada ◽

Michio Ohta

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Gel Electrophoresis ◽

High Performance ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Tandem Mass ◽

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Electroblotting of polypeptides onto glass fiber filters for direct sequence analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Electrophoresis ◽

10.1002/elps.1150110707 ◽

1990 ◽

Vol 11 (7) ◽

pp. 569-572 ◽

Author(s):

Tomas Bergman ◽

Hans Jörnvall

Keyword(s):

Sequence Analysis ◽

Sodium Dodecyl Sulfate ◽

Glass Fiber ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Direct Sequence ◽

Dodecyl Sulfate ◽

Direct Sequence Analysis

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Gels in Vacuo? A Minimalist Approach for Combining Mass Spectrometry and Polyacrylamide Gel Electrophoresis

Mass Spectrometry of Biological Materials ◽

10.1201/9781482269987-18 ◽

1998 ◽

pp. 341-360

Keyword(s):

Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Minimalist Approach

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Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

Methods in Molecular Biology - Protein Electrophoresis ◽

10.1007/978-1-61779-821-4_44 ◽

2012 ◽

pp. 497-509

Author(s):

Wei-Tao Cong ◽

Xu Wang ◽

Sun-Young Hwang ◽

Li-Tai Jin ◽

Jung-Kap Choi

Keyword(s):

Mass Spectrometry ◽

Sodium Dodecyl Sulfate ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Polyacrylamide Gel ◽

Two Dimensional ◽

Dodecyl Sulfate ◽

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Methods in Functional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients In-Gel Digestion and Identification of Proteins by Mass Spectrometry

MAP Kinase Signaling Protocols ◽

10.1385/1-59259-671-1:263 ◽

2004 ◽

pp. 263-282 ◽

Author(s):

Karine R. Bernard ◽

Karen R. Jonscher ◽

Katheryn A. Resing ◽

Natalie G. Ahn

Keyword(s):

Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Functional Proteomics ◽

Two Dimensional ◽

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Isolation and characterization of β-cyclodextrin sulfates by preparative gradient polyacrylamide gel electrophoresis, capillary electrophoresis and electrospray ionization—mass spectrometry

Electrophoresis ◽

10.1002/elps.1150191517 ◽

1998 ◽

Vol 19 (15) ◽

pp. 2677-2681 ◽

Author(s):

Ronald E. Hileman ◽

Marshall M. Siegel ◽

Keiko Tabei ◽

Kuberan Balagurunathan ◽

Robert J. Linhardt

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Polyacrylamide Gel ◽

Ionization Mass ◽

Isolation And Characterization

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