Improving Sample Treatment for In-Solution Protein Identification by Peptide Mass Fingerprint Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2007 ◽  
Vol 6 (9) ◽  
pp. 3393-3399 ◽  
Author(s):  
H. M. Santos ◽  
R. Rial-Otero ◽  
L. Fernandes ◽  
G. Vale ◽  
M. G. Rivas ◽  
...  
Keyword(s):  
Protein Identification ◽  
Laser Desorption ◽  
Sample Treatment ◽  
Peptide Mass Fingerprint ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Identification of Polypeptides Expressed in Response to Vinyl Chloride, Ethene, and Epoxyethane in Nocardioides sp. Strain JS614 by Using Peptide Mass Fingerprinting

2007 ◽  
Vol 73 (13) ◽  
pp. 4368-4372 ◽  
Author(s):  
Adina S. Chuang ◽  
Timothy E. Mattes
Keyword(s):  
Vinyl Chloride ◽  
Laser Desorption ◽  
Peptide Mass Fingerprinting ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.

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