A Comparative Proteomic Study of Human Skin Suction Blister Fluid from Healthy Individuals Using Immunodepletion and iTRAQ Labeling

2012 ◽  
Vol 11 (7) ◽  
pp. 3715-3727 ◽  
Author(s):  
André C. Müller ◽  
Florian P. Breitwieser ◽  
Heinz Fischer ◽  
Christopher Schuster ◽  
Oliver Brandt ◽  
...  
Life Sciences ◽  
1981 ◽  
Vol 29 (4) ◽  
pp. 391-396 ◽  
Author(s):  
Aarne Oikarinen ◽  
Lasse Viinikka ◽  
Heikki Rytsälä ◽  
Urpo Kiistala ◽  
Olavi Ylikorkala

2006 ◽  
Vol 31 (3) ◽  
pp. 445-448 ◽  
Author(s):  
N. Macdonald ◽  
M. Cumberbatch ◽  
M. Singh ◽  
J. G. Moggs ◽  
G. Orphanides ◽  
...  

1996 ◽  
Vol 6 (SUPPLEMENT 1) ◽  
pp. S52
Author(s):  
L. Skov ◽  
H. Hansen ◽  
M. Allen ◽  
J. N. W. N. Barker ◽  
J. Simon ◽  
...  

1983 ◽  
Vol 108 (3) ◽  
pp. 295-299 ◽  
Author(s):  
J.R. BJERKE ◽  
J.K. LIVDEN ◽  
M. DEGRE ◽  
R. MATRE

Dermatology ◽  
1993 ◽  
Vol 186 (1) ◽  
pp. 45-49 ◽  
Author(s):  
R.E. Schopf ◽  
J. Dobmeyer ◽  
T. Dobmeyer ◽  
B. Morsches

1980 ◽  
Vol 26 (13) ◽  
pp. 1825-1828 ◽  
Author(s):  
M J Herfst ◽  
P M Edelbroek ◽  
F A de Wolff

Abstract A method is described for determination of 8-methoxypsoralen to 0.2 mL of suction-blister fluid or 1 mL of serum from psoriatic patients being treated with this drug. The drug is extracted from the biological matrix at pH 9.0 with a mixture of dichloromethane and light petroleum ether. 5-Methoxypsoralen is used as internal standard. Separation and quantitation are performed on a “high-performance” liquid chromatograph with use of an RP 18 reversed-phase column and detection at 245 nm. Accuracy and precision are good. Some benzodiazepines and their metabolites interfere. The lowest detectable concentration is 10 microgram/L, which means that the method is sufficiently sensitive to measure the drug concentrations in serum and suction-blister fluid for pharmacokinetic studies in patients being treated with a therapeutic dosage.


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