Proteomic Analysis ofTrypanosoma cruziSecretome: Characterization of Two Populations of Extracellular Vesicles and Soluble Proteins

2013 ◽  
Vol 12 (2) ◽  
pp. 883-897 ◽  
Author(s):  
Ethel Bayer-Santos ◽  
Clemente Aguilar-Bonavides ◽  
Silas Pessini Rodrigues ◽  
Esteban Maurício Cordero ◽  
Alexandre Ferreira Marques ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Proteomic Analysis ◽  
Soluble Proteins ◽  
Two Populations

Characterization of extracellular vesicles derived from two populations of human placenta derived mesenchymal stem/stromal cells

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S50
Author(s):  
R. Khanabdali ◽  
M. Shojaee ◽  
J. Johnson ◽  
S. Law ◽  
M. Whitmore ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Human Placenta ◽  
Stromal Cells ◽  
Two Populations

Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles

2020 ◽  
Vol 236 ◽  
pp. 111264 ◽  
Author(s):  
Desalegn Woldeyohannes Kifle ◽  
Mark S. Pearson ◽  
Luke Becker ◽  
Darren Pickering ◽  
Alex Loukas ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Extracellular Vesicles ◽  
Proteomic Analysis ◽  
Two Populations

Identification and Partial Characterization of Two Populations of Prostasomes by a Combination of Dynamic Light Scattering and Proteomic Analysis

2015 ◽  
Vol 248 (6) ◽  
pp. 991-1004 ◽  
Author(s):  
Davide Chiasserini ◽  
Michela Mazzoni ◽  
Federico Bordi ◽  
Simona Sennato ◽  
Federica Susta ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Proteomic Analysis ◽  
Partial Characterization ◽  
Two Populations

scholarly journals 109 Isolation and characterization of myometrium and decidual cell-derived extracellular vesicles

2021 ◽  
Vol 224 (2) ◽  
pp. S75-S76
Author(s):  
Megan Shepherd ◽  
Enkhtuya Radnaa ◽  
Rheanna Urrabaz-Garza ◽  
Talar Kechichian ◽  
Ourlad Alzeus G. Tantengco ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Decidual Cell ◽  
Isolation And Characterization

scholarly journals Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 371-381 ◽  
Author(s):  
Vijaya Sunkara ◽  
Hyun-Kyung Woo ◽  
Yoon-Kyoung Cho
Keyword(s):  
Extracellular Vesicles ◽  
Cancer Diagnostics ◽  
Clinical Implementation ◽  
Characterization Methods ◽  
Cancer Management ◽  
Isolation And Characterization

We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.

scholarly journals Characterization of Storage Proteins in Wild (Glycine soja) and Cultivated (Glycine max) Soybean Seeds Using Proteomic Analysis

2006 ◽  
Vol 54 (8) ◽  
pp. 3114-3120 ◽  
Author(s):  
Savithiry S. Natarajan ◽  
Chenping Xu ◽  
Hanhong Bae ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Glycine Max ◽  
Proteomic Analysis ◽  
Storage Proteins ◽  
Glycine Soja ◽  
Soybean Seeds

Proteomic Profile of Urinary Extracellular Vesicles Identifies AGP1 as a Potential Biomarker of Primary Aldosteronism

Endocrinology ◽  
2021 ◽  
Vol 162 (4) ◽  
Author(s):  
Eric R Barros ◽  
Juan Pablo Rigalli ◽  
Alejandra Tapia-Castillo ◽  
Andrea Vecchiola ◽  
Morag J Young ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Primary Aldosteronism ◽  
Proteomic Analysis ◽  
Characteristic Curve ◽  
Area Under The Curve ◽  
Extracellular Vesicle ◽  
Parent Cell ◽  
Spot Urine ◽  
Potential Biomarker ◽  
Aldosterone To Renin Ratio

Abstract Context Primary aldosteronism (PA) represents 6% to 10% of all essential hypertension patients and is diagnosed using the aldosterone-to-renin ratio (ARR) and confirmatory studies. The complexity of PA diagnosis encourages the identification of novel PA biomarkers. Urinary extracellular vesicles (uEVs) are a potential source of biomarkers, considering that their cargo reflects the content of the parent cell. Objective We aimed to evaluate the proteome of uEVs from PA patients and identify potential biomarker candidates for PA. Methods Second morning spot urine was collected from healthy controls (n = 8) and PA patients (n = 7). The uEVs were isolated by ultracentrifugation and characterized. Proteomic analysis on uEVs was performed using LC-MS Orbitrap. Results Isolated uEVs carried extracellular vesicle markers, showed a round shape and sizes between 50 and 150 nm. The concentration of uEVs showed a direct correlation with urinary creatinine (r = 0.6357; P = 0.0128). The uEV size mean (167 ± 6 vs 183 ± 4nm) and mode (137 ± 7 vs 171 ± 11nm) was significantly smaller in PA patients than in control subjects, but similar in concentration. Proteomic analysis of uEVs from PA patients identified an upregulation of alpha-1-acid glycoprotein 1 (AGP1) in PA uEVs, which was confirmed using immunoblot. A receiver operating characteristic curve analysis showed an area under the curve of 0.92 (0.82 to 1; P = 0.0055). Conclusion Proteomic and further immunoblot analyses of uEVs highlights AGP1 as potential biomarker for PA.

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