The Physical Gene Hsp70 Map on Polytene Chromosomes of Anopheles darlingi from the Brazilian Amazon

Genetica ◽  
2004 ◽  
Vol 121 (1) ◽  
pp. 89-94 ◽  
Author(s):  
Míriam Silva Rafael ◽  
Wanderli Pedro Tadei ◽  
Fiona F. Hunter
2016 ◽  
Vol 111 (5) ◽  
pp. 335-346 ◽  
Author(s):  
Anthony J Cornel ◽  
Katherine K Brisco ◽  
Wanderli P Tadei ◽  
Nágila FC Secundino ◽  
Miriam S Rafael ◽  
...  

2020 ◽  
Vol 5 (4) ◽  
pp. 161
Author(s):  
Elerson Matos Rocha ◽  
Ricardo de Melo Katak ◽  
Juan Campos de Oliveira ◽  
Maisa da Silva Araujo ◽  
Bianca Cechetto Carlos ◽  
...  

In Brazil, malaria transmission is mostly confined to the Amazon, where substantial progress has been made towards disease control in the past decade. Vector control has been historically considered a fundamental part of the main malaria control programs implemented in Brazil. However, the conventional vector-control tools have been insufficient to control or eliminate local vector populations due to the complexity of the Amazonian rainforest environment and ecological features of malaria vector species in the Amazon, especially Anopheles darlingi. Malaria elimination in Brazil and worldwide eradication will require a combination of conventional and new approaches that takes into account the regional specificities of vector populations and malaria transmission dynamics. Here we present an overview on both conventional and novel promising vector-focused tools to curb malaria transmission in the Brazilian Amazon. If well designed and employed, vector-based approaches may improve the implementation of malaria-control programs, particularly in remote or difficult-to-access areas and in regions where existing interventions have been unable to eliminate disease transmission. However, much effort still has to be put into research expanding the knowledge of neotropical malaria vectors to set the steppingstones for the optimization of conventional and development of innovative vector-control tools.


1989 ◽  
Vol 84 (4) ◽  
pp. 501-514 ◽  
Author(s):  
Ricardo Lourenço-de-Oliveira ◽  
Anthony Erico da Gama Guimarães ◽  
Monique Arlé ◽  
Tereza Fernandes da Silva ◽  
Márcia Gonçalves Castro ◽  
...  

In view of recent studies incriminating several species of anophelines, besides Anopheles darlingi, as malaria vectors in the Brazilian Amazon, we performed an anopheline survey in four localities - Ariquemes, Cujubim, Machadinho and Itapoã do Oeste - in Rondônia, the most malarious State in the Country. Twenty species were found. An. darlingi was, by far, the dominant species and the only one whose density coincided with that of malaria. On human baits it was more numerous in the immediate vincinity of houses than indoors whre, however, it was almost the only species encountered. On both situations it fed mostly at sunset and during the first half of the night. It was less numerous far from houses and scarce inside the forest. Other species (An. triannulatus, An. evansae, An. albitarsis, An. strodei) appeared in appreciable numbers only in Ariquemes, both in areas with and without malaria. The remaining species were scanty. An. darlingi was confirmed as the primary local vector.


2012 ◽  
Vol 11 (1) ◽  
pp. 351 ◽  
Author(s):  
Keillen M Martins-Campos ◽  
Waléria D Pinheiro ◽  
Sheila Vítor-Silva ◽  
André M Siqueira ◽  
Gisely C Melo ◽  
...  

2009 ◽  
Vol 8 (1) ◽  
pp. 121 ◽  
Author(s):  
Bruno Andrade ◽  
Bruno Rocha ◽  
Antonio Reis-Filho ◽  
Luís Camargo ◽  
Wanderli Tadei ◽  
...  

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e22388 ◽  
Author(s):  
Fábio Saito Monteiro de Barros ◽  
Nildimar Alves Honório ◽  
Mércia Eliane Arruda

2021 ◽  
Author(s):  
Erian Santos ◽  
Ana Cecília Feio dos Santos ◽  
Luciana Letícia da Costa Pires ◽  
Samir Mansour Moraes Casseb ◽  
Gustavo Moraes Holanda ◽  
...  

Abstract Background: Antimicrobial peptides (AMPs) are proteins of the innate immune system that can limit infections of the malaria-carrying parasite Plasmodium, which develops inside anopheline mosquitoes, the human malaria vectors. Despite this, studies on Brazilian Amazon species of anopheline mosquitoes are still needed. The aim of the study is to develop a standard molecular polymerase chain reaction (PCR) technique to detect the AMPs cecropin A (CecA) and defensin from Anopheles darlingi to support studies involving their detection and amplification and better understanding of the roles of these peptides in the Anopheles-Plasmodium interaction. Methods: The collection of anopheline mosquitoes was carried out in three municipalities in the Brazilian Amazon: Altamira and Peixe-Boi, in the state of Pará, and Cruzeiro do Sul, in the state of Acre. The primers were built based on the sequences available in GenBank, and PCR followed standard protocols with different annealing temperatures tested. The PCR products were purified and then sequenced by the dideoxy chain termination method. Results: CecA and defensin amplification were standardized with annealing temperatures of 59ºC and 55ºC, respectively. The amplified products and sequencing demonstrated the good quality of both primer sets. Conclusions: For the first time, a standardized molecular technique for detecting AMPs was described in An. darlingi, a mosquito species from the Brazilian Amazon, supporting future studies aiming to understand the interactions of this species and the action of these peptides during infection and providing important molecular markers for the control of human malaria.


Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 164
Author(s):  
Míriam Silva Rafael ◽  
Leticia Cegatti Bridi ◽  
Igor V. Sharakhov ◽  
Osvaldo Marinotti ◽  
Maria V. Sharakhova ◽  
...  

The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.


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